公开(公告)号：US20220389502A1

公开(公告)日：2022-12-08

申请号：US17832316

申请日：2022-06-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Eathen Ryan ,  Bintian Zhang ,  Xu Wang

IPC: C12Q1/6869 ,  C12Q1/00 ,  G01N27/12

Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure relates to methods for sequencing a polynucleotide using a bioelectronic device that includes protein assemblies used as coupling molecules in bioelectronic circuits. The present disclosure also provides multimeric protein assemblies with various combinations of live and dead subunits arranged to maximize conduction.

公开(公告)号：US20210389296A1

公开(公告)日：2021-12-16

申请号：US17460005

申请日：2021-08-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Peiming Zhang ,  Sovan Biswas ,  Suman Sen ,  Stuart Lindsay

IPC: G01N33/487 ,  C12Q1/6869 ,  C07C321/10

Abstract: The present disclosure provides apparatus and methods for determining the sequence of a nucleic acid. The apparatus comprises electrodes that form a tunnel gap through which the nucleic acid can pass. The electrodes comprise a reagent that is capable of selectively interacting with a nucleobase of the nucleic acid sequence. When the reagent interacts with a nucleobase, a detectable signal is produced and used to identify the nucleobase of the nucleic acid. Advantageously, the apparatus of this disclosure is specific to identifying nucleic acids.

公开(公告)号：US20210247347A1

公开(公告)日：2021-08-12

申请号：US17173569

申请日：2021-02-11

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay ,  Joshua Sadar ,  Quan Qing

IPC: G01N27/327 ,  G01N27/02

Abstract: The present disclosure provides devices, systems, and methods related to protein bioelectronics. In particular, the present disclosure provides bioelectronic devices, systems, and methods that utilize a defined electrical potential to maximize electrical conductance of a protein-of-interest, which can serve as a basis for the fabrication of enhanced bioelectronic devices for the direct measurement of protein activity.

公开(公告)号：US10288599B2

公开(公告)日：2019-05-14

申请号：US15002828

申请日：2016-01-21

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Brett Gyarfas ,  Stuart Lindsay ,  Pei Pang

IPC: G01N33/487 ,  G03F7/20 ,  C12Q1/6869 ,  G01N27/447

Abstract: Embodiments of the disclosure are directed to a device for molecule sensing. In some embodiments, the device includes a first electrode separated from a second electrode by a dielectric layer. The first electrode comprises a large area electrode and the second electrode comprises a small area electrode. At least one opening (e.g., trench) cut or otherwise created into the dielectric layer exposes a tunnel junction therebetween whereby target molecules in solution can bind across the tunnel junction.

公开(公告)号：US10156572B2

公开(公告)日：2018-12-18

申请号：US15119549

申请日：2015-02-18

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Peiming Zhang ,  Stuart Lindsay ,  Subhadip Senapati ,  Sudipta Biswas

IPC: C12Q1/68 ,  G01N33/58 ,  C07C247/04 ,  C07D495/04 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01Q60/38

Abstract: Embodiments of the present disclosure provide bisbiotin ligands and related conjugates and methods. The bisbiotin ligands, combined with streptavidin, can be used in the separation, labelling, targeting, and immobilization of biomolecules.

公开(公告)号：US20240345065A1

公开(公告)日：2024-10-17

申请号：US18604115

申请日：2024-03-13

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Yanan Zhao

IPC: G01N33/487 ,  G01N27/327

CPC classification number: G01N33/48721 ,  G01N27/3276 ,  G01N27/3278

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length L1 and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of L1 and L2.

公开(公告)号：US11959905B2

公开(公告)日：2024-04-16

申请号：US18178130

申请日：2023-03-03

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Yanan Zhao

IPC: G01N27/327 ,  G01N33/487

CPC classification number: G01N33/48721 ,  G01N27/3276 ,  G01N27/3278

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of LI and L2.

公开(公告)号：US20230243807A1

公开(公告)日：2023-08-03

申请号：US18178130

申请日：2023-03-03

Applicant: ARIZONA BOARD OF REGENTS ON behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Yanan Zhao

IPC: G01N27/327

CPC classification number: G01N27/3276

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of LI and L2.

公开(公告)号：US20220196646A1

公开(公告)日：2022-06-23

申请号：US17553392

申请日：2021-12-16

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay ,  Eathen Ryan

IPC: G01N33/543 ,  G01N27/04

Abstract: The present disclosure provides devices, systems, and methods related to protein bioelectronics. In particular, the present disclosure provides devices, systems, and methods for forming electrical contacts to a protein with high yield, which facilitates the manufacture of analytical devices to detect and measure the electrical characteristics corresponding to protein function.

公开(公告)号：US11325987B2

公开(公告)日：2022-05-10

申请号：US16155972

申请日：2018-10-10

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Peiming Zhang ,  Xu Wang ,  Jong One Im ,  Stuart Lindsay

IPC: G01N33/48 ,  G01N33/50 ,  C08B37/00 ,  G06N20/10 ,  G16B40/20 ,  G06N3/08 ,  G16B40/10

Abstract: The present disclosure provides a method for identifying and quantifying sulfated glycosaminoglycans, including for example heparin, by passing a sample through nanopores. The glycosaminoglycans sample is measured in microliter quantities, at nanomolar concentrations with detection of impurities below 0.5%, and a dynamic range over five decades of magnitude with a trained machine learning algorithm.