公开(公告)号：US20240229137A9

公开(公告)日：2024-07-11

申请号：US18382797

申请日：2023-10-23

Applicant: Becton, Dickinson and Company

Inventor： Margaret Nakamoto ,  Eleen Shum ,  Nidhanjali BANSAL ,  Christina Chang

IPC: C12Q1/6883 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  G01N33/5308 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

公开(公告)号：US20240132961A1

公开(公告)日：2024-04-25

申请号：US18382797

申请日：2023-10-22

Applicant: Becton, Dickinson and Company

Inventor： Margaret Nakamoto ,  Eleen Shum ,  Nidhanjali BANSAL ,  Christina Chang

IPC: C12Q1/6883 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  G01N33/5308 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

公开(公告)号：US20230348975A1

公开(公告)日：2023-11-02

申请号：US18187488

申请日：2023-03-21

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Katherine Lazaruk ,  Dennis E. Prosen ,  Devon Jensen ,  David Rosenfeld ,  Janice H. Lai ,  James Ghadiali ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6853

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q2531/113 ,  C12Q2563/185

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

公开(公告)号：US20230340566A1

公开(公告)日：2023-10-26

申请号：US18186940

申请日：2023-03-20

Applicant: Becton, Dickinson and Company

Inventor： Christina Chang ,  Eleen Shum

IPC: C12Q1/6806 ,  C12Q1/686

CPC classification number: C12Q1/6806 ,  C12Q1/686

Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end and are subsequently barcoded on the 5′ end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. There are provided, in some embodiments, methods of 5′-based and 3′-based gene expression profiling. Immune repertoire profiling methods are also provided in some embodiments.

公开(公告)号：US20230193372A1

公开(公告)日：2023-06-22

申请号：US18053603

申请日：2022-11-08

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum

IPC: C12Q1/686

CPC classification number: C12Q1/686

Abstract: Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise.

公开(公告)号：US20190338278A1

公开(公告)日：2019-11-07

申请号：US16400886

申请日：2019-05-01

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Christina Fan

IPC: C12N15/10 ,  C12Q1/6816

Abstract: Disclosed herein include systems, methods, compositions, and kits for molecular barcoding on the 5′-end of a nucleic acid target. After barcoding a nucleic acid target using an oligonucleotide barcode comprising a target binding region and a molecular label to generate a barcoded nucleic acid molecule, an oligonucleotide comprising a complement of the target binding region can be added to generate a barcoded nucleic acid molecule comprising the target-binding region and the complement of the target-binding region. A stem loop is formed with intra-molecular hybridization of the barcoded nucleic acid molecule, which can be extended to generate an extended barcoded nucleic acid molecule comprising the molecular label and a complement of the molecular label.