公开(公告)号：US11661625B2

公开(公告)日：2023-05-30

申请号：US17320052

申请日：2021-05-13

Applicant: Becton, Dickinson and Company

Inventor： Devon Jensen ,  Katherine Lazaruk ,  Dennis Prosen ,  Ricelle A. Acob ,  Kai Liu

IPC: C12Q1/68 ,  C12Q1/6806 ,  C07K14/705 ,  C07K14/725 ,  C12Q1/6858

CPC classification number: C12Q1/6806 ,  C07K14/7051 ,  C07K14/70503 ,  C12Q1/6858

Abstract: Disclosed herein include systems, methods, compositions, and kits for immune repertoire profiling. There are provided, in some embodiments, primer panels enabling the determination of the nucleotide sequence of the complete variable region of nucleic acids encoding mouse B cell receptor (BCR) and T cell receptor (TCR) polypeptides. In some embodiments, the method comprises single cell transcriptomic analysis.

公开(公告)号：US20200255888A1

公开(公告)日：2020-08-13

申请号：US16788221

申请日：2020-02-11

Applicant: Becton, Dickinson and Company

Inventor： Devon Jensen ,  David Rosenfeld ,  Christina Fan ,  Jue Fan

IPC: C12Q1/6809 ,  C12Q1/686 ,  G16B30/00 ,  G16B50/30

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining numbers of occurrences of variants (e.g., transcript variants) of targets (e.g., gene targets) in cells and/or samples. In some embodiments, modification target sites (e.g., polyadenylation sites) and usage thereof are determined. Whole transcriptome amplification analysis can be performed and the sequencing reads obtained can be analyzed to identify polyadenylation sites (and usage thereof) for the design of customized primer panels for targeted scRNAseq experiments.

公开(公告)号：US20210355484A1

公开(公告)日：2021-11-18

申请号：US17320052

申请日：2021-05-13

Applicant: Becton, Dickinson and Company

Inventor： Devon Jensen ,  Katherine Lazaruk ,  Dennis Prosen ,  Ricelle A. Acob ,  Kai Liu

IPC: C12N15/10 ,  C12Q1/6858 ,  C07K14/705 ,  C07K14/725

Abstract: Disclosed herein include systems, methods, compositions, and kits for immune repertoire profiling. There are provided, in some embodiments, primer panels enabling the determination of the nucleotide sequence of the complete variable region of nucleic acids encoding mouse B cell receptor (BCR) and T cell receptor (TCR) polypeptides. In some embodiments, the method comprises single cell transcriptomic analysis.

公开(公告)号：US20230348975A1

公开(公告)日：2023-11-02

申请号：US18187488

申请日：2023-03-21

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Katherine Lazaruk ,  Dennis E. Prosen ,  Devon Jensen ,  David Rosenfeld ,  Janice H. Lai ,  James Ghadiali ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6853

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q2531/113 ,  C12Q2563/185

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

公开(公告)号：US20230272454A1

公开(公告)日：2023-08-31

申请号：US18190465

申请日：2023-03-27

Applicant: Becton, Dickinson and Company

Inventor： Devon Jensen ,  Katherine Lazaruk ,  Dennis Prosen ,  Ricelle A. Acob ,  Kai Liu

IPC: C12Q1/6806 ,  C07K14/705 ,  C07K14/725 ,  C12Q1/6858

CPC classification number: C12Q1/6806 ,  C07K14/70503 ,  C07K14/7051 ,  C12Q1/6858

Abstract: Disclosed herein include systems, methods, compositions, and kits for immune repertoire profiling. There are provided, in some embodiments, primer panels enabling the determination of the nucleotide sequence of the complete variable region of nucleic acids encoding mouse B cell receptor (BCR) and T cell receptor (TCR) polypeptides. In some embodiments, the method comprises single cell transcriptomic analysis.