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公开(公告)号：US20230057188A1
公开(公告)日：2023-02-23
申请号：US17955060
申请日：2022-09-28
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Jonathan Lin , Matthew Bahr , Keegan Owsley
Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.
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公开(公告)号：US20220317018A1
公开(公告)日：2022-10-06
申请号：US17845294
申请日：2022-06-21
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , David A. Roberts
Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.
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公开(公告)号：US11327003B2
公开(公告)日：2022-05-10
申请号：US16557539
申请日：2019-08-30
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Jonathan Lin , Joseph Trotter , Keegan Owsley
Abstract: Non-parametric transforms such as t-distributed stochastic neighbor embedding (tSNE) are used to analyze multi-parametric data such as data derived from flow cytometry or other particle analysis systems and methods. These transforms may be included for dimensionality reduction and identification of subpopulations (e.g., gating). By nature, non-parametric transforms cannot transform new observations without training a new transformation based on the entire dataset including the new observations. The features described parameterize non-parametric transforms using a neural network thereby allowing a small training dataset to be transformed using non-parametric techniques. The training dataset may then be used to generate an accurate parametric model for assessing additional events in a manner consistent with the initial events.
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公开(公告)号：US20200378882A1
公开(公告)日：2020-12-03
申请号：US16887538
申请日：2020-05-29
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US20200309664A1
公开(公告)日：2020-10-01
申请号：US16817331
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Eric D. Diebold , Jonathan Lin , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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Patent Agency Ranking
公开(公告)号：US20240410813A1
公开(公告)日：2024-12-12
申请号：US18746850
申请日：2024-06-18
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan O. Owsley , Jonathan Lin
IPC: G01N15/1434 , G01N15/10 , G01N15/14 , G01N15/1429 , G01N15/149 , G01N21/64
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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公开(公告)号：US20240019354A1
公开(公告)日：2024-01-18
申请号：US18373487
申请日：2023-09-27
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14
CPC classification number: G01N15/1459 , G01N15/1434 , G01N15/1425 , G01N15/1429 , G01N2015/1486 , G01N2015/1402 , G01N2015/142 , G01N2015/149
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US11774343B2
公开(公告)日：2023-10-03
申请号：US17386192
申请日：2021-07-27
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
CPC classification number: G01N15/1434 , G01N15/147 , G01N15/1429 , G01N21/6402 , G01N21/6428 , G01N2015/1006 , G01N2015/149 , G01N2015/1447 , G01N2021/6439
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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公开(公告)号：US11674881B2
公开(公告)日：2023-06-13
申请号：US17845294
申请日：2022-06-21
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , David A. Roberts
IPC: G01N15/14 , G06F18/23 , G06F18/213 , G01N15/10
CPC classification number: G01N15/1429 , G01N15/1456 , G01N15/1459 , G06F18/213 , G06F18/23 , G01N15/1404 , G01N2015/1006 , G01N2015/1402 , G01N2015/149 , G01N2015/1477 , G01N2015/1488
Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.
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公开(公告)号：US20230076378A1
公开(公告)日：2023-03-09
申请号：US17953976
申请日：2022-09-27
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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