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    Parameters for use in particle discrimination
    3.
    发明授权
    Parameters for use in particle discrimination 有权

    公开(公告)号：US11988589B2

    公开(公告)日：2024-05-21

    申请号：US17588040

    申请日：2022-01-28

    Applicant: Becton, Dickinson and Company

    Inventor： Matthew Bahr ,  Eric D. Diebold ,  Jonathan Lin ,  Keegan Owsley

    IPC: G01N15/0227 ,  G01N15/1404

    CPC classification number: G01N15/0227 ,  G01N15/1404

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    Dual excitation beams for irradiating a sample in a flow stream and methods for using same
    4.
    发明授权
    Dual excitation beams for irradiating a sample in a flow stream and methods for using same 有权

    公开(公告)号：US11971343B2

    公开(公告)日：2024-04-30

    申请号：US18142462

    申请日：2023-05-02

    Applicant: Becton, Dickinson and Company

    Inventor： Jizuo Zou ,  Jorge Manzarraga ,  Eric D. Diebold

    IPC: G01N15/14 ,  G01N15/1434 ,  G01N15/10

    CPC classification number: G01N15/1436 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1438

    Abstract: Aspects of the disclosure include methods for generating angularly deflected laser beams for irradiating a sample in a flow stream. Methods according to certain embodiments include generating a first set of angularly deflected laser beams and a second set of angularly deflected laser beams, propagating the first set of angularly deflected laser beams along a different optical path from the second set of angularly deflected laser beams, combining the first set of angularly deflected laser beams with the second set of angularly deflected laser beams and directing the combined sets of laser beams onto a sample in a flow stream and detecting light from the sample. Systems having a laser, an acousto-optic device and an optical adjustment component configured to generate a first set of angularly deflected laser beams and a second set of angularly deflected laser beams are also described.

    Multi-Modal Fluorescence Imaging Flow Cytometry System
    6.
    发明公开
    Multi-Modal Fluorescence Imaging Flow Cytometry System 审中-公开

    公开(公告)号：US20230288311A1

    公开(公告)日：2023-09-14

    申请号：US18198478

    申请日：2023-05-17

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G02B21/06

    CPC classification number: G01N15/1434 ,  G01N15/14 ,  G01N15/1425 ,  G01N15/147 ,  G01N15/1475 ,  G01N15/1429 ,  G01N21/645 ,  G02B21/06 ,  G01N2015/1447 ,  G01N2015/1454 ,  G01N2015/1006

    Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

    Dual Excitation Beams for Irradiating a Sample in a Flow Stream and Methods for Using Same
    7.
    发明公开
    Dual Excitation Beams for Irradiating a Sample in a Flow Stream and Methods for Using Same 审中-公开

    公开(公告)号：US20230273111A1

    公开(公告)日：2023-08-31

    申请号：US18142462

    申请日：2023-05-02

    Applicant: Becton, Dickinson and Company

    Inventor： Jizuo Zou ,  Jorge Manzarraga ,  Eric D. Diebold

    IPC: G01N15/14

    CPC classification number: G01N15/1436 ,  G01N15/1459 ,  G01N2015/1006

    Abstract: Aspects of the disclosure include methods for generating angularly deflected laser beams for irradiating a sample in a flow stream. Methods according to certain embodiments include generating a first set of angularly deflected laser beams and a second set of angularly deflected laser beams, propagating the first set of angularly deflected laser beams along a different optical path from the second set of angularly deflected laser beams, combining the first set of angularly deflected laser beams with the second set of angularly deflected laser beams and directing the combined sets of laser beams onto a sample in a flow stream and detecting light from the sample. Systems having a laser, an acousto-optic device and an optical adjustment component configured to generate a first set of angularly deflected laser beams and a second set of angularly deflected laser beams are also described.

    Cell sorting using a high throughput fluorescence flow cytometer
    8.
    发明授权
    Cell sorting using a high throughput fluorescence flow cytometer 有权

    公开(公告)号：US11105728B2

    公开(公告)日：2021-08-31

    申请号：US16814394

    申请日：2020-03-10

    Applicant: Becton, Dickinson and Company

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G01N15/10

    Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

    Multi-Modal Fluorescence Imaging Flow Cytometry System
    10.
    发明公开
    Multi-Modal Fluorescence Imaging Flow Cytometry System 审中-公开

    公开(公告)号：US20230333002A1

    公开(公告)日：2023-10-19

    申请号：US18214317

    申请日：2023-06-26

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G02B21/06 ,  G01N15/10

    CPC classification number: G01N15/1434 ,  G01N15/1475 ,  G01N15/1425 ,  G01N15/147 ,  G01N15/1429 ,  G01N21/645 ,  G01N15/14 ,  G02B21/06 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2021/6482 ,  G01N2015/1447

    Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

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