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    Dip-stick western blot
    22.
    发明授权
    Dip-stick western blot 有权

    公开(公告)号：US10107780B2

    公开(公告)日：2018-10-23

    申请号：US14644527

    申请日：2015-03-11

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： William Strong

    IPC: G01N27/447 ,  G01N33/543 ,  G01N30/90

    Abstract: Methods, kits, and systems are provided for separating, immobilizing, and/or detecting analytes of one or more samples using dipsticks. A ‘dipstick’ is an object that can be embedded and subsequently removed from a separation medium, and to which analytes can be immobilized while the object is embedded in the separation medium. Examples of separation media include an electrophoresis gel of any format and a stationary phase for column chromatography. Embodiments of the present methods include applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick.

    TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS
    24.
    发明申请
    TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS 审中-公开

    公开(公告)号：US20170241947A1

    公开(公告)日：2017-08-24

    申请号：US15430927

    申请日：2017-02-13

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong

    IPC: G01N27/447 ,  G01N33/543

    CPC classification number: G01N27/44739 ,  G01N27/44747 ,  G01N33/54306 ,  G01N33/54393

    Abstract: Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.

    PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS
    26.
    发明申请
    PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS 有权
    使用改良的环糊精进行蛋白质检测

    公开(公告)号：US20150044705A1

    公开(公告)日：2015-02-12

    申请号：US14455609

    申请日：2014-08-08

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong ,  Lee Olech ,  Thomas Berkelman ,  Christopher Belisle

    IPC: G01N33/53 ,  G01N27/447

    CPC classification number: G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

    Abstract: A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.

    Abstract translation: 提供了一种使用共价连接至少一个标签的环糊精来检测蛋白质的方法。 环糊精可以通过在其疏水腔中螯合蛋白质的芳香族氨基酸侧链而与蛋白质结合。 将蛋白质与环糊精接触后，可以直接检测标记物，或者可以与试剂进行化学相互作用以形成可检测的产物。 标记可以包括吲哚部分，其可以在暴露于紫外光时与卤素取代的有机化合物反应，从而变成荧光。 或者，标记可以包括生物素部分，其可以结合结合配偶体，例如抗生物素蛋白或其变体，以形成可检测的分子复合物。 标记的环糊精可用于本方法中以检测电泳凝胶或印迹膜上的目的蛋白质。 蛋白质，特别是色氨酸的芳香族氨基酸残基由于环糊精的螯合而保护免受化学修饰，使得这些方法与需要完整蛋白质的下游应用相容。 本文还提供了用于检测蛋白质的组合物，试剂盒和电泳凝胶。

    FLUORESCENCE IMAGER ON A MOBILE DEVICE
    27.
    发明申请
    FLUORESCENCE IMAGER ON A MOBILE DEVICE 审中-公开
    荧光成像在移动设备上

    公开(公告)号：US20140312247A1

    公开(公告)日：2014-10-23

    申请号：US14252406

    申请日：2014-04-14

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong ,  Neeraj Bhatt

    IPC: G01N21/64

    CPC classification number: G01N21/6428 ,  G01N21/6456

    Abstract: Systems and methods for substantially simultaneously exciting a fluorescently labeled specimen and capturing fluorescent light emitted therefrom using a smartphone, tablet, or similar mobile computing device, are disclosed herein. The system includes a light-emitting diode (“LED”) light source coupled with the smartphone to excite the specimen and an imaging device coupled with the smartphone to capture fluorescent light emitted from the specimen. The system further includes a hood adapted to be coupled with the smartphone that has an excitation filter configured to produce a first wavelength of electromagnetic radiation to strike the specimen when light from the LED light source passes through it and an emission filter configured to receive the light emitted from the specimen and to produce a second wavelength of electromagnetic radiation to be captured by the imaging device.

    Abstract translation: 本文公开了用于基本上同时激发荧光标记的样本并且使用智能电话，平板电脑或类似的移动计算设备捕获从其发射的荧光的系统和方法。 该系统包括与智能手机耦合以激发样本的发光二极管（“LED”）光源和与智能手机耦合的成像装置以捕获从样品发射的荧光。 该系统还包括适于与智能电话耦合的罩，其具有激励滤波器，该激励滤波器被配置为产生第一波长的电磁辐射以在来自LED光源的光穿过其时撞击样本;以及发射滤波器，被配置为接收光 从样品发射并产生将被成像装置捕获的第二波长的电磁辐射。

    PROTEIN QUANTITATION DEVICE
    30.
    发明申请
    PROTEIN QUANTITATION DEVICE 审中-公开

    公开(公告)号：US20190107484A1

    公开(公告)日：2019-04-11

    申请号：US16151732

    申请日：2018-10-04

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Evan Thrush ,  Steven Swihart ,  William Strong ,  Trey Marlowe

    IPC: G01N21/31 ,  G01N21/3577 ,  G01N21/3563

    Abstract: Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.

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