公开(公告)号：US11565259B2

公开(公告)日：2023-01-31

申请号：US16683798

申请日：2019-11-14

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: B01L3/00 ,  G01N33/558

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US20220388003A1

公开(公告)日：2022-12-08

申请号：US17656244

申请日：2022-03-24

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC: B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US20220379320A1

公开(公告)日：2022-12-01

申请号：US17654938

申请日：2022-03-15

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Eric D. Hobbs ,  Steven W. Short ,  Mark P. White ,  Daniele Malleo

IPC: B03C5/00 ,  B03C5/02 ,  B03C11/00 ,  B01L3/00

Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

公开(公告)号：US11318479B2

公开(公告)日：2022-05-03

申请号：US15105849

申请日：2014-12-18

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Eric D. Hobbs ,  Steven W. Short ,  Mark P. White ,  Daniele Malleo

IPC: C12Q1/68 ,  B03C5/00 ,  B03C5/02 ,  B03C11/00 ,  B01L3/00

Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

公开(公告)号：US11192107B2

公开(公告)日：2021-12-07

申请号：US15306355

申请日：2015-04-25

Applicant: Berkeley Lights, Inc.

Inventor： Igor Y. Khandros ,  J. Tanner Nevill ,  Steven W. Short ,  Ming C. Wu

IPC: B03C5/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/12 ,  B03C5/02 ,  C12M1/02 ,  C12M1/00 ,  C12M1/42

Abstract: A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing an effective wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.

公开(公告)号：US10245588B2

公开(公告)日：2019-04-02

申请号：US15637159

申请日：2017-06-29

Applicant: Berkeley Lights, Inc.

Inventor： Igor Y. Khandros ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Ming C. Wu

IPC: G01N27/447 ,  G01N27/453 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02

Abstract: A structure for providing a boundary for a chamber in a microfluidic apparatus can comprise dielectrophoresis (DEP) configurations each having an outer surface and electrowetting (EW) configurations each having an electrowetting surface. The DEP configurations can facilitate generating net DEP forces with respect to the outer surfaces of the DEP configurations to move micro-objects on the outer surfaces, and the EW configurations can facilitate changing wetting properties of the electrowetting surfaces to move droplets of liquid medium on the electrowetting surfaces.

公开(公告)号：US20180272350A1

公开(公告)日：2018-09-27

申请号：US15843122

申请日：2017-12-15

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC: B01L3/00 ,  B03C5/02 ,  B03C5/00 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  G01N33/68

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/1484 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54313 ,  G01N33/6854

Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US09403172B2

公开(公告)日：2016-08-02

申请号：US14051004

申请日：2013-10-10

Applicant: Berkeley Lights, Inc.

Inventor： Steven W. Short ,  Ming C. Wu

IPC: B03C5/00 ,  B03C5/02 ,  B01L3/00

CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2400/0424 ,  B03C5/026 ,  B03C2201/26

Abstract: A microfluidic optoelectronic tweezers (OET) device can comprise dielectrophoresis (DEP) electrodes that can be activated and deactivated by controlling a beam of light directed onto photosensitive elements that are disposed in locations that are spaced apart from the DEP electrodes. The photosensitive elements can be photodiodes, which can switch the switch mechanisms that connect the DEP electrodes to a power electrode between an off state and an on state.

Abstract translation: 微流体光电镊子（OET）装置可以包括介电电泳（DEP）电极，其可以通过控制定向到设置在与DEP电极间隔开的位置的感光元件上的光束而被激活和去激活。 感光元件可以是光电二极管，其可以将连接DEP电极的开关机构切换到关闭状态和导通状态之间的功率电极。

公开(公告)号：US20150306599A1

公开(公告)日：2015-10-29

申请号：US14262200

申请日：2014-04-25

Applicant: Berkeley Lights, Inc.

Inventor： Igor Y. Khandros ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Ming C. Wu

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: A structure for providing a boundary for a chamber in a microfluidic apparatus can comprise dielectrophoresis (DEP) configurations each having an outer surface and electrowetting (EW) configurations each having an electrowetting surface. The DEP configurations can facilitate generating net DEP forces with respect to the outer surfaces of the DEP configurations to move micro-objects on the outer surfaces, and the EW configurations can facilitate changing wetting properties of the electrowetting surfaces to move droplets of liquid medium on the electrowetting surfaces.

Abstract translation: 用于在微流体装置中提供腔室边界的结构可以包括各自具有每个具有电润湿表面的外表面和电润湿（EW）构造的介电电泳（DEP）构造。 DEP配置可以促进相对于DEP配置的外表面产生净DEP力以移动外表面上的微物体，并且EW配置可以促进电润湿表面的润湿性能的变化，从而将液体介质的液滴移动到 电润湿表面。