公开(公告)号：US20230347347A1

公开(公告)日：2023-11-02

申请号：US18068207

申请日：2022-12-19

申请人： Berkeley Lights, Inc.

发明人： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC分类号： B01L3/00

CPC分类号： B01L3/502761 ,  B01L2300/087 ,  B01L2300/0816 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2200/0652 ,  G01N2469/20

摘要： A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US11365381B2

公开(公告)日：2022-06-21

申请号：US16920365

申请日：2020-07-02

申请人： Berkeley Lights, Inc.

发明人： Randall D. Lowe, Jr. ,  Kristin G. Beaumont ,  Aathavan Karunakaran ,  Natalie C. Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC分类号： C12M3/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00

摘要： Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20220143612A1

公开(公告)日：2022-05-12

申请号：US17085185

申请日：2020-10-30

申请人： Berkeley Lights, Inc.

发明人： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC分类号： B01L3/00 ,  G01N1/40

摘要： A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

公开(公告)号：US10252907B2

公开(公告)日：2019-04-09

申请号：US15439506

申请日：2017-02-22

申请人： Berkeley Lights, Inc.

发明人： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC分类号： B81B7/02 ,  B03C5/00 ,  B03C5/02 ,  G01N27/447 ,  G01N33/50 ,  B03C7/02 ,  G01N33/543 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  G01N35/10

摘要： A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

公开(公告)号：US20160199837A1

公开(公告)日：2016-07-14

申请号：US14965721

申请日：2015-12-10

申请人： Berkeley Lights, Inc.

发明人： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC分类号： B01L3/00 ,  G01N1/40

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2001/4083

摘要： A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

摘要翻译： 提供了一种微流体装置，其具有一个或多个隔离笔，其被配置为通过相对于诸如重力的全局有效力改变微流体装置的取向来隔离一个或多个目标微物体。 还提供了使用重力在微流体装置中选择性地引导微物体运动的方法。 微物体可以是生物微物体，例如细胞，或无生命的微物体，例如珠粒。

公开(公告)号：US20220356429A1

公开(公告)日：2022-11-10

申请号：US17743318

申请日：2022-05-12

申请人： Berkeley Lights, Inc.

发明人： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC分类号： C12M3/06 ,  C12M1/00

摘要： Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US11305283B2

公开(公告)日：2022-04-19

申请号：US16509166

申请日：2019-07-11

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US10376886B2

公开(公告)日：2019-08-13

申请号：US15843122

申请日：2017-12-15

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US09889445B2

公开(公告)日：2018-02-13

申请号：US14521447

申请日：2014-10-22

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M Jimena Loureiro

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

CPC分类号： B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/1484 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54313 ,  G01N33/6854

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US09744533B2

公开(公告)日：2017-08-29

申请号：US14965721

申请日：2015-12-10

申请人： Berkeley Lights, Inc.

发明人： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC分类号： B01L3/00 ,  G01N1/40

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2001/4083

摘要： A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.