公开(公告)号：US20150377861A1

公开(公告)日：2015-12-31

申请号：US14768154

申请日：2014-02-14

Applicant: CFD RESEARCH CORPORATION

Inventor： Kapil Pant ,  Balabhaskar Prabhakarpandian

IPC: G01N33/50 ,  C12M1/36 ,  C12M1/34 ,  C12M3/06 ,  C12M1/00

CPC classification number: G01N33/5008 ,  B01L3/5025 ,  B01L3/502715 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0874 ,  B01L2400/0487 ,  C12M23/16 ,  C12M23/34 ,  C12M25/14 ,  C12M29/00 ,  C12M29/04 ,  C12M35/08 ,  C12M41/00 ,  C12M41/48

Abstract: A cell culture assay device can include: a substrate having a plurality of discrete microfluidic networks and a plurality of wells over the discrete microfluidic networks, each discrete microfluidic network having one or more wells fluidly coupled thereto, the wells extending upward from the discrete microfluidic networks; and a manifold body coupled with the substrate and having at least one fluid conduit pair for each microfluidic network and/or each well, each fluid conduit pair including a fluid inlet conduit and a fluid outlet conduit fluidly coupled to a corresponding microfluidic network and/or well. The substrate can be formed from a substrate base having the microfluidic networks coupled to a well plate having the wells associated with the microfluidic networks.

Abstract translation: 细胞培养测定装置可以包括：具有多个离散的微流体网络的衬底和离散的微流体网络上的多个阱，每个离散微流体网络具有一个或多个与其流体耦合的阱，所述阱从离散微流体网络向上延伸 ; 以及与所述基底联接并具有用于每个微流体网络和/或每个孔的至少一个流体导管对的歧管主体，每个流体导管对包括流体连接到相应的微流体网络的流体入口导管和流体出口导管，和/或 好。 衬底可以由具有耦合到具有与微流体网络相关联的阱的阱板的微流体网络的衬底基底形成。

公开(公告)号：US08940494B2

公开(公告)日：2015-01-27

申请号：US13743171

申请日：2013-01-16

Applicant: CFD Research Corporation

Inventor： Balabhaskar Prabhakarpandian ,  Kapil Pant

IPC: G01N33/554 ,  C12Q1/02 ,  G01N33/50 ,  G01N33/543 ,  G01N33/569

CPC classification number: C12Q1/025 ,  G01N33/5008 ,  G01N33/5029 ,  G01N33/5032 ,  G01N33/5047 ,  G01N33/54366 ,  G01N33/56972

Abstract: Methods of assaying the leukocyte adhesion cascade (LAC) and monitoring leukocyte rolling, adhesion, and/or migration can be implemented with an apparatus that includes an idealized microvascular network (IMN) of one or more interconnected idealized flow channels in fluid communication through a porous wall with a tissue space (e.g., idealized tissue space). The methods of assaying the LAC can be implemented with means for quantifying modulation of the leukocyte adhesion cascade. Methods of assaying the LAC can be implemented with the device and one or more active agents to monitor leukocyte rolling, adhesion, and/or migration in the presence of absence of the active agent. Migration can be through the idealized flow channels, through the porous wall, and/or into the tissue space.

Abstract translation: 测定白细胞粘附级联（LAC）和监测白细胞滚动，粘附和/或迁移的方法可以用包括理想的微血管网络（IMN）的装置来实现，所述设备包括一个或多个互连的理想化流动通道，其通过多孔 具有组织空间的壁（例如，理想化的组织空间）。 可以用用于量化白细胞粘附级联调节的方法来实施测定LAC的方法。 可以使用该装置和一种或多种活性剂来实施测定LAC的方法，以在存在活性剂的情况下监测白细胞的滚动，粘附和/或迁移。 迁移可以通过理想的流动通道，通过多孔壁和/或进入组织空间。

公开(公告)号：US20140255961A1

公开(公告)日：2014-09-11

申请号：US14202721

申请日：2014-03-10

Applicant: CFD Research Corporation

Inventor： Balabhaskar Prabhakarpandian ,  Kapil Pant

IPC: G01N33/50 ,  G01N33/86

CPC classification number: G01N33/5029 ,  B01L3/5027 ,  G01N33/5005 ,  G01N33/5011 ,  G01N33/5061 ,  G01N33/5088 ,  G01N33/86 ,  G01N2333/4703 ,  G01N2333/70525

Abstract: A method of assaying wound healing can include: growing cells on the matrix in the first flow channel; introducing an agent that removes the matrix from the junction; introducing a matrix material into the second flow channel so as to form the second matrix in the second flow channel and junction; and detecting cellular migration into the junction onto the second matrix. The agent that removes the matrix can include a biomolecule or chemical agent. The method can include removing cells in the matrix in the junction before introducing the matrix material into the second flow channel. A bioactive agent can be introduced into the junction to determine if it modulates cellular migration and/or clot formation into the intersection openings of tissue and vascular channels.

Abstract translation: 测定伤口愈合的方法可以包括：在第一流动通道中在基质上生长细胞; 引入从接合处去除基质的试剂; 将基质材料引入到第二流动通道中，以便在第二流动通道和结中形成第二基质; 并检测细胞迁移到第二基质上的结。 去除基质的试剂可以包括生物分子或化学试剂。 该方法可以包括在将基质材料引入第二流动通道之前去除接合中的基体中的细胞。 可以将生物活性剂引入接头以确定其是否调节细胞迁移和/或凝块形成到组织和血管通道的交叉开口中。