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公开(公告)号：US20210269868A1
公开(公告)日：2021-09-02
申请号：US17187267
申请日：2021-02-26
Applicant: CFD Research Corporation
IPC: C12Q1/6853 , C12Q1/6832 , C12Q1/6816
Abstract: A primer composition for amplifying a target nucleic acid of a plurality of target organisms can include: a first primer having in order: 3′-a first target specific sequence; a melt key region; and a first super primer region, wherein the first target specific sequence includes a nucleic acid sequence that specifically hybridizes with a first unique nucleic acid sequence of a first target organism over nucleic acid sequences of other organisms, the melt key region includes a nucleic acid sequence that has less than full identity with a melt key probe and a complement of the melt key region hybridizes with the melt key probe with at least one nucleotide mismatch with the melt key probe, and the first super primer region includes a nucleic acid sequence that is common to the plurality of target organisms.
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公开(公告)号：US20210116338A1
公开(公告)日：2021-04-22
申请号：US17074007
申请日：2020-10-19
Applicant: CFD RESEARCH CORPORATION
Abstract: A fluidic device can include: a plurality of fluid conduits, each fluid conduit including a first conduit portion separated from a second conduit portion; and at least one transport body that is movably positioned between the first conduit portion and the second conduit portion of each fluid conduit. The at least one transport body can include: at least one port adapted to be aligned with a first conduit portion and a second conduit portion of at least one first conduit so as to fluidly couple the first conduit portion with the second conduit portion; and at least one blocking body portion adapted to be aligned with a first conduit portion and a second conduit portion of at least one second conduit so as to fluidly isolate the first conduit portion from the second conduit portion of the at least one second conduit.
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公开(公告)号：US10012640B2
公开(公告)日：2018-07-03
申请号：US14768154
申请日：2014-02-14
Applicant: CFD Research Corporation
Inventor： Kapil Pant , Balabhaskar Prabhakarpandian
IPC: C12M1/00 , C12M3/00 , B01L3/00 , G01N33/50 , C12M3/06 , C12M1/12 , C12M1/34 , C12M1/36 , C12M1/42
CPC classification number: G01N33/5008 , B01L3/5025 , B01L3/502715 , B01L2300/0829 , B01L2300/0861 , B01L2300/0874 , B01L2400/0487 , C12M23/16 , C12M23/34 , C12M25/14 , C12M29/00 , C12M29/04 , C12M35/08 , C12M41/00 , C12M41/48
Abstract: A cell culture assay device can include: a substrate having a plurality of discrete microfluidic networks and a plurality of wells over the discrete microfluidic networks, each discrete microfluidic network having one or more wells fluidly coupled thereto, the wells extending upward from the discrete microfluidic networks; and a manifold body coupled with the substrate and having at least one fluid conduit pair for each microfluidic network and/or each well, each fluid conduit pair including a fluid inlet conduit and a fluid outlet conduit fluidly coupled to a corresponding microfluidic network and/or well. The substrate can be formed from a substrate base having the microfluidic networks coupled to a well plate having the wells associated with the microfluidic networks.
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公开(公告)号：US20140255970A1
公开(公告)日：2014-09-11
申请号：US14202491
申请日：2014-03-10
Applicant: CFD Research Corporation
Inventor： Balabhaskar Prabhakarpandian , Kapil Pant
IPC: G01N33/50
CPC classification number: G01N33/5029 , B01L3/5027 , G01N33/5005 , G01N33/5011 , G01N33/5061 , G01N33/5088 , G01N33/86 , G01N2333/4703 , G01N2333/70525
Abstract: A cell culture device can include: an internal chamber configured for an internal cell culture that has at least one port coupled to a perfusion modulating device capable of modulating perfusion in the internal chamber; at least one fluid channel bordering the internal chamber that is configured for a channel cell culture that has at least one port coupled to a perfusion modulating device capable of modulating perfusion in the fluid channel; and a wall separating the internal chamber and at least one fluid channel having gaps that fluidly couple the internal chamber with the at least one fluid channel, wherein the perfusion modulating device causes reduced fluid flow. The internal chamber can include a first cell type and the at least one fluid channel includes a second cell type. The first cell type has an ischemic zone in the middle, a non-ischemic zone adjacent with the at least one fluid channel, and a border zone between the ischemic zone and non-ischemic zone. In one aspect, the internal chamber and at least one fluid channel are modeled from physiological features. In one aspect, the internal chamber and at least one fluid channel are modeled from idealized features.
Abstract translation: 细胞培养装置可以包括：配置用于内部细胞培养物的内部室，其具有耦合到能够调节内部腔室灌注的灌注调节装置的至少一个端口; 至少一个与所述内室邻接的流体通道，其构造成用于通道细胞培养，所述通道细胞培养物具有至少一个端口，所述至少一个端口耦合到能够调节所述流体通道中的灌注的灌注调节装置; 以及分隔内室和至少一个流体通道的壁，所述至少一个流体通道具有使内室与至少一个流体通道流体连接的间隙，其中灌注调节装置引起减少的流体流动。 内部腔室可以包括第一细胞类型，并且所述至少一个流体通道包括第二细胞类型。 第一细胞类型在中间具有缺血区，与至少一个流体通道相邻的非缺血区，以及缺血区与非缺血区之间的边界区。 在一个方面，内部室和至少一个流体通道由生理特征建模。 在一个方面，内部室和至少一个流体通道由理想特征建模。
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公开(公告)号：US20240197166A1
公开(公告)日：2024-06-20
申请号：US18542002
申请日：2023-12-15
Applicant: CFD Research Corporation
CPC classification number: A61B3/0025 , G06T7/0012 , G06T15/00 , G16H50/20 , G16H50/50 , G06T2207/30024 , G06T2207/30041
Abstract: The method involves computationally modeling a cornea by simulating physical cornea models with computational in silico models. The simulated data is compared with actual data from the physical models, and the simulation is iterated until a match is achieved. The method may also include calibrating diffusion parameters for testing substances, and configuring the models for analyzing instances of fluid-induced shear and substance transport. The simulated data may be used to obtain corneal pharmacokinetic models, and the method may be used to obtain predictions for time-dependent concentrations of molecules in the eye or systemic circulation. The computational models may consider instances of convective-diffusive transport and carrier-mediated transport in calculations and determinations of transport characteristics and values of different testing substances.
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Patent Agency Ranking
公开(公告)号：US20240161653A1
公开(公告)日：2024-05-16
申请号：US18511347
申请日：2023-11-16
Applicant: CFD Research Corporation
Inventor： Carrie Lynn German , Balabhaskar Prabhakarpandian , Ketan Harendrakumar Bhatt , Ashley Gilbert Helser
IPC: G09B23/28
CPC classification number: G09B23/281
Abstract: The method involves computationally modeling a placenta by simulating physical placenta models with computational in silico models. The simulated data is compared with actual data from the physical models, and the simulation is iterated until a match is achieved. The method may also include calibrating diffusion parameters for testing substances, and configuring the models for analyzing instances of fluid-induced shear and substance transport. The simulated data may be used to obtain maternal-fetal pharmacokinetic in silico placenta models, and the method may be used to obtain predictions for time-dependent concentrations of molecules in fetal circulation. The computational models may consider instances of fluid-diffusive transport and carrier-mediated transport in calculations and determinations of transport characteristics and values of different testing substances.
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公开(公告)号：US20210346584A1
公开(公告)日：2021-11-11
申请号：US17313830
申请日：2021-05-06
Applicant: CFD Research Corporation
Abstract: A portable platelet apheresis system can include: a whole blood inlet configured to receive whole blood; an anticoagulant source containing an anticoagulant; a mixer fluidly coupled with the whole blood inlet and anticoagulant source and configured to mix the whole blood and the anticoagulant; a whole blood sorter microfluidic network; a platelet poor outlet positioned to receive a platelet poor fraction; and a platelet concentrator outlet positioned to receive a concentrated platelet fraction. The whole blood sorter microfluidic network includes: a sorter constricted region having a first cross-sectional dimension; a sorter expansion region having a second cross-sectional dimension that is larger than the first cross-sectional dimension; at least one sorter side channel (platelet rich plasma channel) formed into a side of the sorter expansion region; and at least one sorter outlet (platelet poor plasma channel) that is downstream or medial from the at least one sorter side channel.
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公开(公告)号：US10775364B2
公开(公告)日：2020-09-15
申请号：US15017125
申请日：2016-02-05
Applicant: CFD Research Corporation
Inventor： Balabhaskar Prabhakarpandian , Kapil Pant
Abstract: A method of inducing ischemia includes: providing a cell culture device having a first cell culture in an internal chamber and a second cell culture in at least one fluid channel and a perfusion modulating system that causes changes in oxygen flow in the internal chamber and/or at least one fluid channel; flowing liquid media having oxygen through the internal chamber and at least one fluid channel of the cell culture device; modulating oxygen perfusion in the internal chamber with the perfusion modulating system by varying and selectively blocking the flow rate of at least one of the liquid media or oxygen through the internal chamber to induce varying levels of ischemia; and assaying for ischemia in the first cell culture.
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公开(公告)号：US09933413B2
公开(公告)日：2018-04-03
申请号：US14202511
申请日：2014-03-10
Applicant: CFD Research Corporation
Inventor： Balabhaskar Prabhakarpandian , Kapil Pant
CPC classification number: G01N33/5029 , B01L3/5027 , G01N33/5005 , G01N33/5011 , G01N33/5061 , G01N33/5088 , G01N33/86 , G01N2333/4703 , G01N2333/70525
Abstract: A method of assaying metastasis can include: providing a device of one of the embodiments; introducing the at least one cancer cell into the at least one internal chamber or at least one fluid channel; and studying metastasis of the at least one cancer cell. Optionally: introducing cancer cells into a first internal chamber; detecting escape of the cancer cell from the first internal chamber into the fluid channel; detecting migration of the cancer cell through the fluid channel; detecting adhesion of the cancer cell to a coating on the fluid channel; detecting invasion of the cancer cell into a second internal chamber from the fluid channel; or visualizing metastasis of the cancer cell with a visualization device.
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公开(公告)号：US20160153970A1
公开(公告)日：2016-06-02
申请号：US15017125
申请日：2016-02-05
Applicant: CFD Research Corporation
Inventor： Balabhaskar Prabhakarpandian , Kapil Pant
IPC: G01N33/50
Abstract: A cell culture device can include: an internal chamber configured for an internal cell culture that has at least one port coupled to a perfusion modulating device capable of modulating perfusion in the internal chamber; at least one fluid channel bordering the internal chamber that is configured for a channel cell culture that has at least one port coupled to a perfusion modulating device capable of modulating perfusion in the fluid channel; and a wall separating the internal chamber and at least one fluid channel having gaps that fluidly couple the internal chamber with the at least one fluid channel, wherein the perfusion modulating device causes reduced fluid flow. The internal chamber can include a first cell type and the at least one fluid channel includes a second cell type. The first cell type has an ischemic zone in the middle, a non-ischemic zone adjacent with the at least one fluid channel, and a border zone between the ischemic zone and non-ischemic zone. In one aspect, the internal chamber and at least one fluid channel are modeled from physiological features. In one aspect, the internal chamber and at least one fluid channel are modeled from idealized features.
Abstract translation: 细胞培养装置可以包括：配置用于内部细胞培养物的内部室，其具有耦合到能够调节内部腔室灌注的灌注调节装置的至少一个端口; 至少一个与所述内室邻接的流体通道，其构造成用于通道细胞培养，所述通道细胞培养物具有至少一个端口，所述至少一个端口耦合到能够调节所述流体通道中的灌注的灌注调节装置; 以及分隔内室和至少一个流体通道的壁，所述至少一个流体通道具有使内室与至少一个流体通道流体连接的间隙，其中灌注调节装置引起减少的流体流动。 内部腔室可以包括第一细胞类型，并且所述至少一个流体通道包括第二细胞类型。 第一细胞类型在中间具有缺血区，与至少一个流体通道相邻的非缺血区，以及缺血区与非缺血区之间的边界区。 在一个方面，内部室和至少一个流体通道由生理特征建模。 在一个方面，内部室和至少一个流体通道由理想特征建模。
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