公开(公告)号：US11149287B2

公开(公告)日：2021-10-19

申请号：US16246864

申请日：2019-01-14

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US20200224269A1

公开(公告)日：2020-07-16

申请号：US16819972

申请日：2020-03-16

Applicant: Dow AgroSciences LLC

Inventor： PoHao Wang ,  Shreedharan Sriram ,  Lakshmi Sastry-Dent

IPC: C12Q1/6876 ,  G16B25/00 ,  C12Q1/6895 ,  C12N15/82 ,  G16B20/00

Abstract: The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold value for predicting gene silencing. The methods and/or systems provided herein can be used in high-throughput setting for screening large number of transformed event in a relatively short period of time as compared to existing technologies.

公开(公告)号：US20190327928A1

公开(公告)日：2019-10-31

申请号：US16460660

申请日：2019-07-02

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Vipula Shukla ,  Manju Gupta ,  Fyodor Urnov ,  Dmitry Y. Guschin ,  Michiel Jan De Both ,  Paul Bundock ,  Lakshmi Sastry-Dent

IPC: A01H6/82 ,  C12N15/82

Abstract: Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

公开(公告)号：US10415046B2

公开(公告)日：2019-09-17

申请号：US15338888

申请日：2016-10-31

Applicant: DOW AGROSCIENCES LLC

Inventor： W. Michael Ainley ,  James W. Bing ,  David R. Corbin ,  Steven L. Evans ,  Joseph F. Petolino ,  Lakshmi Sastry-Dent ,  Steven A. Thompson ,  Steven R. Webb ,  Mary E. Welter ,  Ning Zhou

IPC: C12N15/82 ,  C12N9/12

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

公开(公告)号：US10233465B2

公开(公告)日：2019-03-19

申请号：US14531732

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10106804B2

公开(公告)日：2018-10-23

申请号：US15867770

申请日：2018-01-11

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  W. Michael Ainley

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10087492B2

公开(公告)日：2018-10-02

申请号：US15705447

申请日：2017-09-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael Ainley ,  Jayakumar P. Samuel ,  Zehui Cao ,  Liu Shen ,  Cristie M. Dewes

IPC: C12P19/34 ,  C12Q1/6895

Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

公开(公告)号：US20170088825A1

公开(公告)日：2017-03-30

申请号：US15379152

申请日：2016-12-14

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Vipula Shukla ,  Manju Gupta ,  Fyodor Urnov ,  Dmitry Y. Guschin ,  Michiel Jan De Both ,  Paul Bundock ,  Lakshmi Sastry-Dent

IPC: C12N9/04 ,  A01C14/00 ,  C12N15/82

CPC classification number: C12Y101/01037 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8261 ,  Y02A40/146

Abstract: Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

公开(公告)号：US20130211729A1

公开(公告)日：2013-08-15

申请号：US13761711

申请日：2013-02-07

Applicant: Dow Agrosciences LLC

Inventor： Lakshmi Sastry-Dent ,  Shreedharan Sriram ,  Navin Elango ,  Zehui Cao ,  Karthik Narayan Muthuraman

IPC: G06F19/28

CPC classification number: G16B50/00 ,  G16B30/00

Abstract: Systems and methods for data analysis are provided. In one embodiment, a method for analysis is provided, including electronically receiving sequence data; electronically receiving one or more reference data sequences related to at least an expression vector; associating the sequence data with at least one of the reference data sequences to identify a transgene flanking sequence; searching a genome for one or more insertion sites of the transgene flanking sequence; and annotating the genome and the one or more insertion sites within the genome when one or more insertion sites are found in said searching step.

Abstract translation: 提供了数据分析的系统和方法。 在一个实施例中，提供了一种用于分析的方法，包括电子地接收序列数据; 电子地接收与至少一个表达载体相关的一个或多个参考数据序列; 将序列数据与至少一个参考数据序列相关联以鉴定转基因侧翼序列; 在基因组中搜索转基因侧翼序列的一个或多个插入位点; 并且当在所述搜索步骤中发现一个或多个插入位点时，注释基因组和基因组内的一个或多个插入位点。