公开(公告)号：US20170029833A1

公开(公告)日：2017-02-02

申请号：US15212000

申请日：2016-07-15

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry R. Wright ,  Manju Gupta

IPC: C12N15/82 ,  C07K14/005

CPC classification number: C12N15/8216 ,  C07K14/005 ,  C12N15/8222 ,  C12N15/8286 ,  C12N2770/00022 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 aerie or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1通气或其功能等同物的最小核心启动子元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US09453235B2

公开(公告)日：2016-09-27

申请号：US13674712

申请日：2012-11-12

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US20150173371A1

公开(公告)日：2015-06-25

申请号：US14567574

申请日：2014-12-11

Applicant: Dow AgroSciences LLC

Inventor： Richard K. Mann ,  Robert A. Masters ,  Steve Mcmaster ,  Mark Peterson ,  Monica Sorribas Amela ,  Terry R. Wright

IPC: A01N57/20 ,  A01N37/38

CPC classification number: A01N39/04 ,  A01N33/12 ,  A01N57/20 ,  A01N2300/00

Abstract: Provided herein are herbicidal compositions comprising a mixture comprising (a) a 2,4-D-choline salt and (b) a salt of N-(phosphonomethyl)glycine (glyphosate). The compositions provide synergistic weed control of undesirable vegetation in areas including, but not limited to, non-crop, fallow-bed, perennial crops, tree, vine and fruit orchards, and plantation crops.

Abstract translation: 本文提供了除草组合物，其包含包含（a）2,4-D-胆碱盐和（b）N-（膦酰基甲基）甘氨酸（草甘膦）的盐的混合物。 这些组合物在包括但不限于非作物，休耕，多年生作物，树木，葡萄树和水果果园以及种植园作物的地区中提供不期望的植被的协同杂草控制。

公开(公告)号：US08916752B2

公开(公告)日：2014-12-23

申请号：US13647081

申请日：2012-10-08

Applicant: Dow AgroSciences LLC

Inventor： Terry R. Wright ,  Justin M. Lira ,  Terence Anthony Walsh ,  Donald Merlo ,  Jayakumar P. Samuel ,  Gaofeng Lin

IPC: C12N15/82

CPC classification number: C12N15/8274 ,  C12N9/0071 ,  C12N15/8275

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Abstract translation: 本发明提供了不仅对2,4-D有抗性的新型植物，而且还提供了对吡啶氧基乙酸酯除草剂的抗性。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。 根据本发明使用的优选酶和基因在本文中称为AAD-12（丙烯酰氧基烷酸酯加氧酶）。 这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。

公开(公告)号：US20140023243A1

公开(公告)日：2014-01-23

申请号：US13947137

申请日：2013-07-22

Applicant: Dow Agrosciences LLC

Inventor： Nandi Nagaraj ,  Reetal Pai ,  Pradeep Setlur ,  Terry R. Wright

IPC: G06K9/00

CPC classification number: G06K9/00624 ,  G06K2209/17 ,  G06T7/0012 ,  G06T2207/30188 ,  G06T2207/30242

Abstract: An apparatus and method for determining the number of kernels on a sample cob are provided. In one embodiment, an image including a front region of the sample cob, and a back region of the sample cob displayed in at least one reflective surface are provided to an image processor that identifies the presence of kernels in the image and determines the number of kernels based on the identified presence of kernels in the image.

Abstract translation: 提供了一种用于确定样品芯上的颗粒数量的装置和方法。 在一个实施例中，将包括样品芯的前部区域的图像和在至少一个反射表面中显示的样品芯片的后部区域提供给图像处理器，该图像处理器识别图像中存在的内核，并且确定 基于图像中确定的内核存在的内核。

公开(公告)号：US10036028B2

公开(公告)日：2018-07-31

申请号：US14587757

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v1.

公开(公告)号：US10030246B2

公开(公告)日：2018-07-24

申请号：US14587735

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. B104.

公开(公告)号：US09885054B2

公开(公告)日：2018-02-06

申请号：US15618986

申请日：2017-06-09

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/10 ,  C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.

公开(公告)号：US20180022703A1

公开(公告)日：2018-01-25

申请号：US15671671

申请日：2017-08-08

Applicant: Dow AgroSciences, LLC

Inventor： Norbert M. Satchivi ,  Paul R. Schmitzer ,  Carla N. Yerkes ,  Terry R. Wright

IPC: C07D213/79 ,  A01N57/20 ,  A01N47/36 ,  A01N43/54 ,  A01N43/82 ,  A01N43/653 ,  A01N43/90 ,  A01N43/40 ,  A01N43/707 ,  A01N43/56 ,  A01N43/42 ,  A01N43/88 ,  A01N43/58 ,  A01N25/32 ,  A01N33/12 ,  A01N35/10 ,  A01N37/22 ,  A01N37/38 ,  A01N37/40 ,  A01N39/02 ,  A01N39/04 ,  A01N41/10 ,  A01N43/38 ,  A01N43/70 ,  A01N43/76 ,  A01N43/80 ,  A01N47/12 ,  A01N47/30 ,  A01N47/34 ,  A01N47/38 ,  A01N43/50

Abstract: An herbicidal composition containing (a) a pyridine or pyrimidine carboxylic acid component and (b) a second cereal or rice herbicide component provides synergistic control of selected weeds.

公开(公告)号：US20160002662A1

公开(公告)日：2016-01-07

申请号：US14725328

申请日：2015-05-29

Applicant: DOW AGROSCIENCES LLC

Inventor： Justin M. Lira ,  Erika Snodderley ,  Andrew E. Robinson ,  Terry R. Wright ,  Donald Merlo

IPC: C12N15/82

CPC classification number: C12N15/8274 ,  C12N9/0004 ,  C12N15/8275

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to a pyridyloxyacetate herbicide. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-13 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Abstract translation: 本发明提供了不仅对2,4-D有抗性的新型植物，而且还涉及吡啶氧基乙酸酯除草剂。 本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。 根据本发明使用的优选酶和基因在本文中称为AAD-13（丙烯酰氧基烷酸酯加氧酶）。 这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。