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    Soybean event pDAB9582.816.15.1 detection method
    22.
    发明授权
    Soybean event pDAB9582.816.15.1 detection method 有权

    公开(公告)号：US09863008B2

    公开(公告)日：2018-01-09

    申请号：US14410793

    申请日：2013-06-25

    Applicant: DOW AGROSCIENCES LLC

    Inventor： Lauren Clark ,  Kelley Ann Smith ,  Yang Wang ,  Ning Zhou

    IPC: C12Q1/68 ,  C12N15/82

    CPC classification number: C12Q1/6895 ,  C12N15/8277 ,  C12N15/8286 ,  C12Q2600/13 ,  C12Q2600/156 ,  Y02A40/162

    Abstract: Soybean Event pDAB9582.816.15.1 comprises gene expression cassettes which contain genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The disclosure provides polynucleotide related event detection methods. The present disclosure relates to a method for detecting a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated Soybean Event pDAB9582.816.15,1. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome. SEQ ID NO: 1 and SEQ ID N0:2 are diagnostic for Soybean Event pDAB9582.816.15.1.

    HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS
    24.
    发明申请
    HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS 审中-公开
    转基因边界的高通量分析

    公开(公告)号：US20130130920A1

    公开(公告)日：2013-05-23

    申请号：US13683442

    申请日：2012-11-21

    Applicant: Dow AgroSciences LLC

    Inventor： Zehui Cao ,  Stephen Novak ,  Lakshmi Sastry-Dent ,  Ning Zhou

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6895

    Abstract: A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.

    Abstract translation: 在其中掺入转基因的染色体DNA中分析衍生自与转基因相邻的染色体的DNA侧翼区的方法。 其中DNA侧翼区的特征在于用限制酶分离和消化基因组DNA，将双链衔接子连接到分离和消化的基因组DNA上，衔接子连接的基因组DNA的引物延伸反应和 通过链霉亲和素 - 生物素相互作用引物延伸反应产物。 通过随后的PCR扩增反应和DNA测序进一步表征DNA侧翼区。

    DNA detection methods for site specific nuclease activity
    26.
    发明授权
    DNA detection methods for site specific nuclease activity 有权
    用于位点特异性核酸酶活性的DNA检测方法

    公开(公告)号：US09493844B2

    公开(公告)日：2016-11-15

    申请号：US14105223

    申请日：2013-12-13

    Applicant: Dow AgroSciences LLC

    Inventor： Lakshmi Sastry-Dent ,  Matthew Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  A01G24/00 ,  C12N5/00 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/70 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C40B40/08

    Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

    Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。

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