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公开(公告)号：US08422031B2
公开(公告)日：2013-04-16
申请号：US12947367
申请日：2010-11-16
CPC分类号： G02B21/247 , G02B21/245
摘要： A method for controlling a focus of an optical system. The method includes providing a pair of incident light beams to a conjugate lens. The incident light beams are directed by the lens to converge toward a focal region. The method also includes reflecting the incident light beams with an object positioned proximate to the focal region. The reflected light beams return to and propagate through the lens. The method also includes determining relative separation measured between the reflected light beams and determining a degree-of-focus of the optical system with respect to the sample based upon the relative separation.
摘要翻译： 一种用于控制光学系统的焦点的方法。 该方法包括将一对入射光束提供给共轭透镜。 入射光束被透镜引导以朝向焦点区域会聚。 该方法还包括用邻近聚焦区域定位的物体反射入射光束。 反射光束返回并传播通过透镜。 该方法还包括确定在反射光束之间测量的相对间隔，并基于相对间隔确定光学系统相对于样品的焦度。
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公开(公告)号：US08252250B2
公开(公告)日：2012-08-28
申请号：US12626353
申请日：2009-11-25
申请人： Jonathan Posner , Kamil Salloum , Michal Lebl , Mark Reed , Dale Buermann , Matthew Hage , Bryan Crane , David Heiner , Robert Kain
发明人： Jonathan Posner , Kamil Salloum , Michal Lebl , Mark Reed , Dale Buermann , Matthew Hage , Bryan Crane , David Heiner , Robert Kain
IPC分类号： B01L3/00
CPC分类号： F04B37/10 , F04B19/00 , Y10T436/2575
摘要： An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.
摘要翻译： 提供一种电渗（EO）泵，其包括具有泵腔的壳体，多孔芯介质和电极。 多孔芯介质定位在泵腔内以形成至少部分地围绕多孔芯介质的外表面延伸的外部储存器。 多孔芯介质具有设置在其中的开放内室。 内室表示内部储存器。 电极定位在内室中并且位于外表面附近。 电极通过多孔芯介质在内部和外部储存器之间引导流体流动，其中当电极引起流体流动时产生气体。 壳体具有用于将流体输送到内部储存器和外部储存器中的一个的流体入口。 壳体具有流体出口，以从另一个内部储存器和外部储存器排出流体。 壳体具有用于从泵腔去除气体的气体去除装置。
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公开(公告)号：US20120195794A1
公开(公告)日：2012-08-02
申请号：US13442013
申请日：2012-04-09
申请人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
发明人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
CPC分类号： F04B19/006 , B01L3/502715 , B01L3/50273 , B01L7/00 , B01L2300/0816 , B01L2300/0877 , B01L2400/0418 , G01N35/00613 , G01N35/1095 , Y10T436/2575
摘要： A flow cell for use in a microfluidic detection system is provided. The flow cell includes a flow cell body having a channel that is configured to convey a solution through the flow cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system, and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.
摘要翻译： 提供了一种用于微流体检测系统的流通池。 流动池包括具有通道的流动池主体，该通道被配置为将溶液输送通过流动池主体。 流动池还包括底面和顶面。 底表面被构造成由检测系统可移除地保持，并且顶表面是透明的并且允许光通过。 流动池体还包括与通道流体连通的流体入口和出口。 在流动池主体中还设有泵腔。 泵腔与通道的端部和流体入口和出口之一流体连通并且介于其间。 电渗（EO）泵保持在泵腔中。 EO泵通过EO泵和流体入口和出口之间的通道引起溶液的流动。
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公开(公告)号：US20120149585A1
公开(公告)日：2012-06-14
申请号：US13399820
申请日：2012-02-17
申请人： Wenyi Feng , Jason Bryant , Dale Buermann
发明人： Wenyi Feng , Jason Bryant , Dale Buermann
CPC分类号： C12Q1/6874 , C12Q1/6825 , C12Q1/6869 , G01N21/05 , G01N21/6458 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421 , G02B21/0024 , G02B2207/113
摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。
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公开(公告)号：US08173080B2
公开(公告)日：2012-05-08
申请号：US12031054
申请日：2008-02-14
申请人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
发明人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
IPC分类号： B01L3/00
CPC分类号： F04B19/006 , B01L3/502715 , B01L3/50273 , B01L7/00 , B01L2300/0816 , B01L2300/0877 , B01L2400/0418 , G01N35/00613 , G01N35/1095 , Y10T436/2575
摘要： A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system, and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.
摘要翻译： 提供了一种用于微流体检测系统的流通池。 流动池包括具有通道的流动池主体，该通道被配置为将溶液输送通过流动池主体。 流动池还包括底面和顶面。 底表面被构造成由检测系统可移除地保持，并且顶表面是透明的并且允许光通过。 流动池体还包括与通道流体连通的流体入口和出口。 在流动池主体中还设有泵腔。 泵腔与通道的端部和流体入口和出口之一流体连通并且介于其间。 电渗（EO）泵保持在泵腔中。 EO泵通过EO泵和流体入口和出口之间的通道引起溶液的流动。
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公开(公告)号：US08241573B2
公开(公告)日：2012-08-14
申请号：US12295337
申请日：2007-03-30
申请人： Saibal Banerjee , Colin Barnes , Kevin Benson , Jason Bryant , Dale Buermann , Sergey Etchin , Johnny Ho , Xavier Lee , Peter Lundberg , Klaus Maisinger , Bojan Obradovic , Mark Pratt , Mark Reed , Chiara Rodighiero , Subra Sankar , Harold Swerdlow , Eric Vermaas
发明人： Saibal Banerjee , Colin Barnes , Kevin Benson , Jason Bryant , Dale Buermann , Sergey Etchin , Johnny Ho , Xavier Lee , Peter Lundberg , Klaus Maisinger , Bojan Obradovic , Mark Pratt , Mark Reed , Chiara Rodighiero , Subra Sankar , Harold Swerdlow , Eric Vermaas
CPC分类号： C12Q1/6869 , C12Q2535/122 , C12Q2565/60 , G01N21/6456
摘要： The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.
摘要翻译： 本发明包括用于核酸测序的系统和装置，例如来自克隆扩增的单分子阵列的短DNA序列。
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公开(公告)号：US08143599B2
公开(公告)日：2012-03-27
申请号：US13281237
申请日：2011-10-25
申请人： Wenyi Feng , Jason Bryant , Dale Buermann
发明人： Wenyi Feng , Jason Bryant , Dale Buermann
IPC分类号： G01N21/64
CPC分类号： C12Q1/6874 , C12Q1/6825 , C12Q1/6869 , G01N21/05 , G01N21/6458 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421 , G02B21/0024 , G02B2207/113
摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
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公开(公告)号：US20120040364A1
公开(公告)日：2012-02-16
申请号：US13281237
申请日：2011-10-25
申请人： Wenyi Feng , Jason Bryant , Dale Buermann
发明人： Wenyi Feng , Jason Bryant , Dale Buermann
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6825 , C12Q1/6869 , G01N21/05 , G01N21/6458 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421 , G02B21/0024 , G02B2207/113
摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
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公开(公告)号：US08071962B1
公开(公告)日：2011-12-06
申请号：US13209306
申请日：2011-08-12
申请人： Wenyi Feng , Jason Bryant , Dale Buermann
发明人： Wenyi Feng , Jason Bryant , Dale Buermann
IPC分类号： G01N21/64
CPC分类号： C12Q1/6874 , C12Q1/6825 , C12Q1/6869 , G01N21/05 , G01N21/6458 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421 , G02B21/0024 , G02B2207/113
摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。
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公开(公告)号：US08039817B2
公开(公告)日：2011-10-18
申请号：US12434495
申请日：2009-05-01
申请人： Wenyi Feng , Jason Bryant , Dale Buermann
发明人： Wenyi Feng , Jason Bryant , Dale Buermann
IPC分类号： G01N21/64
CPC分类号： C12Q1/6874 , C12Q1/6825 , C12Q1/6869 , G01N21/05 , G01N21/6458 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421 , G02B21/0024 , G02B2207/113
摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
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