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    APPARATUS FOR FRAGMENTING NUCLEIC ACIDS
    3.
    发明申请
    APPARATUS FOR FRAGMENTING NUCLEIC ACIDS 有权
    装备核酸的装置

    公开(公告)号：US20120292190A1

    公开(公告)日：2012-11-22

    申请号：US13551704

    申请日：2012-07-18

    申请人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

    发明人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

    IPC分类号： C25B7/00

    CPC分类号： F04B37/10 ,  F04B19/00 ,  Y10T436/2575

    摘要： An apparatus for fragmenting nucleic acid. The apparatus includes a sample reservoir that comprises a fluid having nucleic acids. The apparatus can also include a shear wall that is positioned within the sample reservoir. The shear wall includes a porous core medium that has pores that are sized to permit nucleic acids to flow therethrough. The apparatus also includes first and second chambers that are separated by the shear wall. The first and second chambers are in fluid communication with each other through the porous core medium of the shear wall. Also, the apparatus may include first and second electrodes that are located within the first and second chambers, respectively. The first and second electrodes are configured to generate an electric field that induces a flow of the sample fluid. The nucleic acids move through the shear wall thereby fragmenting the nucleic acids.

    摘要翻译： 核酸分裂装置。 该装置包括包含具有核酸的流体的样品储存器。 该装置还可以包括位于样品容器内的剪切壁。 剪切壁包括多孔芯介质，其具有尺寸允许核酸流过其中的孔。 该装置还包括被剪力墙隔开的第一和第二室。 第一和第二腔室通过剪切壁的多孔纤维介质彼此流体连通。 此外，该装置可以包括分别位于第一和第二室内的第一和第二电极。 第一和第二电极被配置成产生诱发样品流体流动的电场。 核酸通过剪切壁移动，从而使核酸分裂。

    High density plate filler
    5.
    发明申请
    High density plate filler 审中-公开
    高密度板填料

    公开(公告)号：US20060272738A1

    公开(公告)日：2006-12-07

    申请号：US11393060

    申请日：2006-03-30

    申请人： Gary Lim ,  Nigel Beard ,  Mark Reed

    发明人： Gary Lim ,  Nigel Beard ,  Mark Reed

    IPC分类号： B67C3/00

    CPC分类号： B01L3/0293 ,  B01L3/0268 ,  B01L2200/021 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0409 ,  G01N35/1074 ,  G01N2035/1039

    摘要： A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries. Each of the plurality of capillaries can comprise an inlet and an outlet. A funnel member can comprise a first assay chamber and a first outlet in fluid communication with the first assay chamber. A first porous member can be disposed at an the first outlet to deliver a first fluid bead of the assay along a top surface of the output layer and in fluid communication with at least some of the plurality of capillaries such that a portion of the fluid bead is drawn within at least some of the plurality of capillaries in response to capillary force. The funnel member and the output layer can be moveable relative to each other between a first position and a second position to draw the first fluid bead across the top surface.

    摘要翻译： 一种用于填充微板的填充装置。 微量培养板具有多个孔，每个孔的大小用于接受测定。 填充装置可以包括具有多个毛细管的输出层。 多个毛细管中的每一个可以包括入口和出口。 漏斗构件可以包括第一测定室和与第一测定室流体连通的第一出口。 第一多孔构件可以设置在第一出口处，以沿着输出层的顶表面递送测定的第一流体珠并与多个毛细管中的至少一些流体连通，使得流体珠的一部分 在多个毛细管中的至少一些中被响应于毛细管力吸入。 漏斗构件和输出层可以在第一位置和第二位置之间相对于彼此移动，以便通过顶表面拉动第一流体珠。

    Detection of gene expression
    6.
    发明申请
    Detection of gene expression 审中-公开
    检测基因表达

    公开(公告)号：US20060141518A1

    公开(公告)日：2006-06-29

    申请号：US11320440

    申请日：2005-12-28

    申请人： Kai Lao ,  Mark Reed

    发明人： Kai Lao ,  Mark Reed

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2537/143 ,  C12Q2549/119 ,  C12Q2561/113

    摘要： Methods for detection of nucleic acids such as a cDNA copy of an mRNA are disclosed. The methods comprise using a PCR to form a preamplification product which comprises cDNA sequence as well as primer target sequences and a detection probe sequence, which are introduced by the forward and reverse primers. In a second PCR, preamplification product is amplified using universal primers which hybridize to the primer target sequences or their complements. Amplification can be detected using a detection probe that hybridizes to the detection probe sequence or its complement.

    摘要翻译： 公开了检测核酸例如mRNA的cDNA拷贝的方法。 所述方法包括使用PCR形成包含cDNA序列以及通过正向和反向引物引入的引物靶序列和检测探针序列的预扩增产物。 在第二次PCR中，使用与引物靶序列或其互补序列杂交的通用引物扩增前扩增产物。 可以使用与检测探针序列或其补体杂交的检测探针来检测扩增。

    Electroosmotic pump with improved gas management
    8.
    发明授权
    Electroosmotic pump with improved gas management 有权
    电渗泵具有改进的气体管理

    公开(公告)号：US08252250B2

    公开(公告)日：2012-08-28

    申请号：US12626353

    申请日：2009-11-25

    申请人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

    发明人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

    IPC分类号： B01L3/00

    CPC分类号： F04B37/10 ,  F04B19/00 ,  Y10T436/2575

    摘要： An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.

    摘要翻译： 提供一种电渗（EO）泵，其包括具有泵腔的壳体，多孔芯介质和电极。 多孔芯介质定位在泵腔内以形成至少部分地围绕多孔芯介质的外表面延伸的外部储存器。 多孔芯介质具有设置在其中的开放内室。 内室表示内部储存器。 电极定位在内室中并且位于外表面附近。 电极通过多孔芯介质在内部和外部储存器之间引导流体流动，其中当电极引起流体流动时产生气体。 壳体具有用于将流体输送到内部储存器和外部储存器中的一个的流体入口。 壳体具有流体出口，以从另一个内部储存器和外部储存器排出流体。 壳体具有用于从泵腔去除气体的气体去除装置。

    CHANNEL ESTIMATION FOR RAPID DISPERSIVE FADING CHANNELS
    10.
    发明申请
    CHANNEL ESTIMATION FOR RAPID DISPERSIVE FADING CHANNELS 审中-公开
    用于快速分散衰减信道的信道估计

    公开(公告)号：US20090103666A1

    公开(公告)日：2009-04-23

    申请号：US12295713

    申请日：2007-03-30

    申请人： Ming Zhao ,  Zhenning Shi ,  Mark Reed

    发明人： Ming Zhao ,  Zhenning Shi ,  Mark Reed

    IPC分类号： H04L27/06

    CPC分类号： H04L25/022 ,  H04L25/023 ,  H04L27/2647

    摘要： This invention addresses the problem of channel estimation in fast fading communications channels, particularly for OFDM systems. It finds wide application in existing and future systems such as WLAN and WiMax. In particular, the invention involves a method of channel estimation and data detection for rapid dispersive fading channels due to high mobility. The invention involves decoding a symbol of the received transmission by retrieving pilot tones from it and using these to estimate variations in the channel frequency response using an iterative maximum likelihood channel estimation process, in which the estimation process comprises the following steps: In a first iteration, deriving soft decoded data information, that is information having a confidence value or reliability associated with it, from the estimates of the channel frequency response for the symbol obtained from pilot tones. And, in at least a second iteration using the soft decoded data information as virtual pilot tones together with the pilot tones to re-estimate the channel frequency response for the symbol. In other aspects the invention concerns a receiver and software designed to perform the method.

    摘要翻译： 本发明解决了在快速衰落通信信道中的信道估计问题，特别是用于OFDM系统。 它在现有和未来的系统中广泛应用，如WLAN和WiMax。 特别地，本发明涉及由于高移动性而对快速色散衰落信道进行信道估计和数据检测的方法。 本发明涉及通过从其中检索导频音来解码所接收的传输的符号，并使用它们来估计信道频率响应的变化，其中估计过程包括以下步骤：在第一次迭代中 根据从导频音获得的符号的信道频率响应的估计，导出软解码数据信息，即具有与其相关联的置信度值或可靠度的信息的软解码数据信息。 并且在至少第二次迭代中，使用软解码数据信息作为虚拟导频音与导频音一起重新估计符号的信道频率响应。 在其它方面，本发明涉及被设计用于执行该方法的接收器和软件。