摘要:
A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.
摘要:
The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
摘要:
A method for assigning an IP address to a terminal device and a communication system. The method includes: a router transmitting to a layer-2 device network a Router Advertisement message carrying routing prefix information; the layer-2 device network generating extended routing prefix information which identifies the downlink port uniquely within the broadcast domain of the subnet where the layer-2 device network is located according to the routing prefix information in the Router Advertisement message, and transmitting to the terminal device a Router Advertisement message carrying the extended routing prefix information; and the terminal device generating its IP address according to the extended routing prefix information in the Router Advertisement message and an Interface ID of the terminal device. Accordingly, it is unnecessary for the terminal device to perform address uniqueness check across the layer-2 devices in the network to ensure uniqueness of the IP address of the terminal device, thus improving the operating performance of the network.
摘要:
A method includes identifying a pair of image data blocks separated by a boundary. The image data blocks include image information defining multiple pixels in at least one image. The method also includes identifying at least one filter length based on edge contents of at least some of the pixels in the at least one image. In addition, the method includes filtering at least some of the pixels in the pair of image data blocks along the boundary using the at least one identified filter length.
摘要:
A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.
摘要:
A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.
摘要:
An electrical current is created across an electrically conductive medium comprising a cell which may be part of a tissue of a living organism. A first electrical parameter which may be current, voltage, or electrical impedance is measured. A second electrical parameter which may be current, voltage or a combination of both is then adjusted and/or analyzed. Adjustments are carried out to facilitate analysis and/or obtain a desired degree of electroporation. Analysis is carried out to determine characteristics of the cell membrane and/or tissue.
摘要:
A variable displacement swash plate type compressor which incorporates a lubrication passage formed in a cylinder block and crankcase, and a spray nozzle disposed at the crankcase end of the lubrication passage. The lubrication passage provides fluid communication between an oil sump and the spray nozzle, and the spray nozzle causes lubricating oil to be efficiently distributed as a fine mist or spray to the crank chamber. The lubrication passage and spray nozzle maximize the flow of refrigerant gas and lubricating oil to the crank chamber under all operating conditions providing cooling and lubrication to the internal moving components within the crankcase.
摘要:
Images created by electrical impedance tomography (EIT) are used to adjust one or more electrical parameters and obtain a desired degree of electroporation of cells in tissue. The parameters include current, voltage and a combination thereof. The cells are subjected to conditions such that they become permeabilized but are preferably not subjected to conditions which result in irreversible pore formation and cell death. The electroporation can analyze cell membranes, diagnose tissues and the patient as well as to move materials into and out of cells in a controlled manner.
摘要:
Embodiments of present invention provide a method of splicing optical fibers. The method includes holding a first and a second fiber respectively by a first and a second rotary clamp; aligning axes of the first and second fibers to a common reference; moving the first and second fibers that are being held by the first and second rotary clamps onto a splicing machine; and causing the first and second fibers being spliced together by the splicing machine. A fiber splicing apparatus or system is also provided for performing the method thereof.