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38 条记录 (耗时 0.025 秒)

    Methodology to produce, and purify and assay polypeptides with the proteolytic activity of the HCV NS3 protease
    21.
    发明授权
    Methodology to produce, and purify and assay polypeptides with the proteolytic activity of the HCV NS3 protease 失效
    用于产生和纯化和测定具有HCV NS3蛋白酶的蛋白水解活性的多肽的方法

    公开(公告)号：US06197536B1

    公开(公告)日：2001-03-06

    申请号：US09011961

    申请日：1998-02-23

    申请人： Christian Steinkühler ,  Antonello Pessi ,  Elisabetta Bianchi ,  Marina Taliani ,  Licia Tomei ,  Andrea Urbani ,  Raffaele De Francesco ,  Frank Narjes

    发明人： Christian Steinkühler ,  Antonello Pessi ,  Elisabetta Bianchi ,  Marina Taliani ,  Licia Tomei ,  Andrea Urbani ,  Raffaele De Francesco ,  Frank Narjes

    IPC分类号： C12Q137

    CPC分类号： C07K14/005 ,  C12N9/506 ,  C12N2770/24222

    摘要： The process according to the present invention allows expression and isolation of polypeptides with the proteolytic activity of HCV NS3 protease in a pure, catalytically active form, and in amounts that are sufficient for discovery of NS3 protease inhibitors and for determination of the three-dimensional structure of the NS3 protease. A further subject of the present invention is a procedure that defines the chemical and physical conditions necessary for completion of the proteolytic activity of the above polypeptides. The invention further comprises new compositions of matter (expression vectors) containing nucleotide sequences capable of expressing the above mentioned polypeptides in culture cells. Finally, new compounds of matter are defined, suitable to measure the above proteolytic activity, and useful to develop NS3 protease inhibitors and therefore therapeutic agents for use against HCV. The figure shows the kinetic parameters of HCV NS3 protease using the S3 depsipeptide substrate (SEQ ID NO:45).

    摘要翻译： 根据本发明的方法允许以纯的，催化活性形式表达和分离具有HCV NS3蛋白酶的蛋白水解活性的多肽，并且其量足以发现NS3蛋白酶抑制剂和用于确定三维结构 的NS3蛋白酶。 本发明的另一主题是定义完成上述多肽的蛋白水解活性所需的化学和物理条件的方法。 本发明还包括含有能够在培养细胞中表达上述多肽的核苷酸序列的物质组合物（表达载体）。 最后，定义新的物质化合物，适合于测量上述蛋白水解活性，并且可用于开发NS3蛋白酶抑制剂，因此用于抗HCV的治疗剂。 该图显示了使用S3 depipipeptide底物（SEQ ID NO：45）的HCV NS3蛋白酶的动力学参数。

    Synthetic, immunologically active peptides useful for the preparation of antimalarial vaccines
    25.
    发明授权
    Synthetic, immunologically active peptides useful for the preparation of antimalarial vaccines 失效
    用于制备抗疟疾疫苗的合成，免疫活性肽

    公开(公告)号：US5116946A

    公开(公告)日：1992-05-26

    申请号：US358932

    申请日：1989-05-30

    申请人： Antonio S. Verdini ,  Antonello Pessi ,  Fabio Bonelli

    发明人： Antonio S. Verdini ,  Antonello Pessi ,  Fabio Bonelli

    IPC分类号： A61K39/00 ,  C07K14/445

    CPC分类号： C07K14/445 ,  A61K39/00

    摘要： Synthetic peptides constituted by at least two repeating, consecutive units of asparaginyl-proline (Asn-Pro).sub.n sequence, are described.The peptides are powerful immunogens, capable of inducing in laboratory animals the formation of a high concentration of antibodies capable of reacting both with the (Asn-Pro).sub.n peptides, and with the immunodominant epitope of the circumsporozoite protein of Plasmodium falciparum.The peptides, which can be obtained in a pure form by chemical synthesis, are particularly useful for preparing antimalarial vaccines and diagnostic kits for the determination of malaria in man.

    摘要翻译： 描述了由至少两个重复连续单位的天冬酰胺酰脯氨酸（Asn-Pro）n序列构成的合成肽。 这些肽是强大的免疫原，能够在实验动物中诱导形成高浓度的能够与（Asn-Pro）n肽反应的抗体，并与恶性疟原虫的环子孢子蛋白的免疫优势表位反应。 可以通过化学合成以纯形式获得的肽特别可用于制备用于测定人类疟疾的抗疟疾疫苗和诊断试剂盒。

    Sequential polypeptides endowed with immunological activity
    29.
    发明授权
    Sequential polypeptides endowed with immunological activity 失效
    具有免疫活性的顺序多肽

    公开(公告)号：US5219987A

    公开(公告)日：1993-06-15

    申请号：US227364

    申请日：1988-08-02

    申请人： Antonio S. Verdini ,  Antonello Pessi ,  Fabio Bonelli

    发明人： Antonio S. Verdini ,  Antonello Pessi ,  Fabio Bonelli

    IPC分类号： A61K39/00 ,  A61K39/015 ,  C07K14/445

    CPC分类号： C07K14/445 ,  A61K39/015 ,  A61K39/00

    摘要： Synthetic polypeptides constituted by at least two consecutive repeating units of (Asn-Asn-Pro) sequence are powerful immunogens in test animals.The formed antibodies recognize both the synthetic polypeptides and the immuno-dominant epitope of the circumsporozoitic protein of Plasmodium falciparum.Said polypeptides, which can be obtained in pure form, are particularly useful for the preparation of antimalarial vaccines and of diagnostic kits for the determination of malarial affections.

    摘要翻译： 由（Asn-Asn-Pro）序列的至少两个连续重复单元构成的合成多肽在测试动物中是强大的免疫原。 形成的抗体识别恶性疟原虫的环孢子虫蛋白的合成多肽和免疫优势表位。 可以以纯形式获得的所述多肽特别可用于制备抗疟疾疫苗和用于测定疟疾感染的诊断试剂盒。

    Method for the determination of anti-P. falciparum sporozoite antibodies in human blood
    30.
    发明授权
    Method for the determination of anti-P. falciparum sporozoite antibodies in human blood 失效
    测定抗P的方法。 人类血液中的恶性疟原虫子孢子抗体

    公开(公告)号：US4977079A

    公开(公告)日：1990-12-11

    申请号：US134229

    申请日：1987-12-17

    申请人： Carlo A. Nuzzolo ,  Adriano Bernardi ,  Antonello Pessi ,  Antonio S. Verdini

    发明人： Carlo A. Nuzzolo ,  Adriano Bernardi ,  Antonello Pessi ,  Antonio S. Verdini

    IPC分类号： G01N33/535 ,  G01N33/569

    CPC分类号： G01N33/56905 ,  G01N33/535 ,  Y10S435/81 ,  Y10S435/947 ,  Y10S436/808 ,  Y10S436/828

    摘要： An immunoenzimatic method is disclosed for the detection and the measurement of anti-P. falciparum sporzoite antibodies in human blood and/or in its derivatives, which operates with a synthetic antigen-enzyme conjugate capable of forming with the antisporozoite antibodies a stable antibody-synthetic antigen-enzyme complex, and one or more proteins absorbed and/or covalently linked to a solid support, which eagerly bind the antisporozoite antibody of said complex.The method, thanks to its simpleness, specificity and rapidity, is particularly useful in epidemiologic investigations into malaria and into the efficacy of an antimalarial vaccine.

    摘要翻译： 公开了用于检测和测量抗-P的免疫学方法。 人类血液中和/或其衍生物中的恶性疟原虫子孢子抗体，其与能够与抗细菌子抗体形成稳定的抗体合成抗原 - 酶复合物的合成抗原 - 酶缀合物一起操作，以及吸收和/或共价连接的一种或多种蛋白质 到固体支持物，其急剧结合所述复合物的反子孢子抗体。 由于其简单，特异性和快速性，该方法在对疟疾的流行病学调查和抗疟疾疫苗的疗效方面特别有用。