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    Nucleotide and amino acid sequences of canine herpesvirus gB and gC
    24.
    发明授权
    Nucleotide and amino acid sequences of canine herpesvirus gB and gC 失效
    犬疱疹病毒gB和gC的核苷酸和氨基酸序列

    公开(公告)号：US5529780A

    公开(公告)日：1996-06-25

    申请号：US220151

    申请日：1994-03-30

    申请人： Enzo Paoletti ,  Keith J. Limbach

    发明人： Enzo Paoletti ,  Keith J. Limbach

    IPC分类号： A61K39/00 ,  C07K14/03 ,  A61K39/245 ,  C12N7/01 ,  C12N15/38 ,  C12P21/02

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/14143 ,  C12N2710/16722

    摘要： Disclosed and claimed are nucleotides for genes encoding the canine herpesvirus (CHV) gB, gC and gD homologues. These genes encode polypeptides of 879, 459 and 345 amino acids, respectively, which are also disclosed. The nucleotides can be expressed in any suitable vector system, allowing for production of the polypeptides. Additionally, the vector system containing any or any combination of the nucleotides can be employed in an antigenic, immunological or vaccine composition, such as a poxvirus vector system, e.g., a CHV-vaccinia or avipox virus recombinant, as can the products from expression, i.e., the gB, gC and gD glycoproteins. Antibodies elicited by the glycoproteins or from expression of the vector containing the nucleotide are also useful. Methods for making and using the composition are also disclosed and claimed.

    摘要翻译： 公开并要求保护的是用于编码犬疱疹病毒（CHV）gB，gC和gD同系物的基因的核苷酸。 这些基因分别编码879,459和345个氨基酸的多肽，其也被公开。 核苷酸可以在任何合适的载体系统中表达，允许产生多肽。 另外，含有任何或任何组合的核苷酸的载体系统可以用于抗原性，免疫学或疫苗组合物，例如痘病毒载体系统，例如CHV-痘苗或Avipox病毒重组体，以及来自表达的产物， 即gB，gC和gD糖蛋白。 由糖蛋白引起的抗体或含有该核苷酸的载体的表达也是有用的。 还公开并要求保护和使用组合物的方法。

    Recombinant poxvirus host range selection system
    25.
    发明授权
    Recombinant poxvirus host range selection system 失效
    重组痘病毒宿主范围选择系统

    公开(公告)号：US5453364A

    公开(公告)日：1995-09-26

    申请号：US102702

    申请日：1993-08-05

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： C12N15/09 ,  A61K39/00 ,  C07K14/03 ,  C07K14/145 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/39 ,  C12N15/863 ,  C12P21/02 ,  C12R1/91 ,  C12N15/86

    CPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12N2710/24143 ,  C12N2760/20122

    摘要： What is described is a modified recombinant virus for expressing a gene product in a host. The modified recombinant virus has host range genes deleted therefrom so that the virus has restricted replication in the host. The modified recombinant virus also contains DNA which codes for and expresses the gene product in the host even with restricted replication of the virus in the host. The modified recombinant virus is used in a method for expressing a gene product in a host or in a cell cultured in vitro, and in a vaccine for inducing an immunological response in a host inoculated with the vaccine. What is also described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conditional lethal mutant (host range) of poxviruses. A deletion/recombinant mutant of the vaccinia virus was generated which is capable of plaquing on primary chick embryo fibroblasts and two monkey cell lines (BSC-40 or VERO) but is defective in replication in the human cell line MRC-5. Insertion of the host range gene into the deletion/recombinant restores the ability for growth on MRC-5 cells. A series of plasmids were constructed which allow for the rapid single-step cloning and expression of any open reading frame when recombined with the deletion/recombinant and scored for growth on MRC-5 cells.

    摘要翻译： 描述的是用于在宿主中表达基因产物的修饰的重组病毒。 修饰的重组病毒具有从其中缺失的宿主范围基因，使得病毒在宿主中具有限制性的复制。 修饰的重组病毒还含有编码并在宿主中表达基因产物的DNA，甚至在宿主中病毒的复制受限。 修饰的重组病毒用于在宿主或体外培养的细胞中表达基因产物的方法，以及用于在接种疫苗的宿主中诱导免疫应答的疫苗。 还描述了在痘病毒，特别是痘苗病毒中克隆和表达开放阅读框的选择系统。 选择系统基于痘病毒的条件致死突变体（宿主范围）。 产生痘苗病毒的缺失/重组突变体，其能够在原代小鸡胚胎成纤维细胞和两种猴细胞系（BSC-40或VERO）上进行染色，但在人细胞系MRC-5中复制缺陷。 将宿主范围基因插入缺失/重组体中恢复MRC-5细胞生长能力。 构建了一系列质粒，当与缺失/重组重组并在MRC-5细胞上进行生长时，允许快速单步克隆和任何开放阅读框的表达。

    Recombinant avipox virus
    27.
    发明授权
    Recombinant avipox virus 失效
    重组禽病毒

    公开(公告)号：US06340462B1

    公开(公告)日：2002-01-22

    申请号：US08455345

    申请日：1995-05-31

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： A61K39275

    CPC分类号： C12N15/86 ,  A61K39/00 ,  C07K14/005 ,  C12N2710/16622 ,  C12N2710/24043 ,  C12N2710/24143 ,  C12N2730/10122 ,  C12N2740/13022 ,  C12N2740/15022 ,  C12N2760/16122 ,  C12N2760/18122 ,  C12N2760/20122 ,  C12N2770/20022

    摘要： The present invention provides a method for inducing an immunological response in a vertebrate to a pathogen by inoculating the vertebrate with a synthetic recombinant avipox virus modified by the presence, in a non-essential region of the avipox genome, of DNA from any source which encodes for and expresses an antigen of the pathogen. The present invention further provides a synthetic recombinant avipox virus modified by the insertion therein of DNA from any source, and particularly from a non-avipox source, into a non-essential region of the avipox genome.

    摘要翻译： 本发明提供了一种通过用合成的重组avipox病毒接种脊椎动物来诱导脊椎动物免疫应答的方法，所述合成重组禽病毒通过在所述avipox基因组的非必需区域中存在的DNA从任何编码的来源 用于表达病原体的抗原。 本发明进一步提供了通过将来自任何来源，特别是非脱脂素源的DNA插入其中的合成的重组禽病毒脂蛋白，进一步转化为该脂蛋白基因组的非必需区域。