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    Nucleotide and amino acid sequences of canine herpesvirus GD and uses therefor
    1.
    发明授权
    Nucleotide and amino acid sequences of canine herpesvirus GD and uses therefor 失效
    犬疱疹病毒GD的核苷酸和氨基酸序列，并用于其

    公开(公告)号：US6017542A

    公开(公告)日：2000-01-25

    申请号：US473446

    申请日：1995-06-07

    申请人： Enzo Paoletti ,  Keith J. Limbach

    发明人： Enzo Paoletti ,  Keith J. Limbach

    IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61P31/12 ,  A61P31/22 ,  C07H21/04 ,  C07K14/03 ,  C12N15/38 ,  C12P21/02 ,  C12N7/01

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/14143 ,  C12N2710/16722

    摘要： Disclosed and claimed are nucleotides for genes encoding the canine herpesvirus (CHV) gB, gC and gD homologues. These genes encode polypeptides of 879, 459 and 345 amino acids, respectively, which are also disclosed and claimed. The genes are useful as DNA probes or, for preparing PCR primers. The polypeptides are useful in antigenic, immunological or vaccine compositions. The nucleotides can be expressed in any suitable vector system, allowing for production of the polypeptides. Additionally, the vector system containing any or any combination of the nucleotides can be employed in an antigenic, immunological or vaccine composition, such as a poxvirus vector system, e.g., a CHV-vaccinia or avipox virus recombinant, as can the products from expression, i.e., the gB, gC and gD glycoproteins. Antibodies elicited by the glycoproteins or from expression of the vector containing the nucleotide(s) are also useful. Methods for making and using the composition are also disclosed and claimed. Also, specific canarypox-CHV gB, gC and gD recombinants vCP 320, vCP322 and vCP294 and methods for making and using them are also disclosed and claimed.

    摘要翻译： 公开并要求保护的是用于编码犬疱疹病毒（CHV）gB，gC和gD同系物的基因的核苷酸。 这些基因分别编码879,459和345个氨基酸的多肽，其也被公开和要求保护。 这些基因可用作DNA探针，或用于制备PCR引物。 多肽可用于抗原性，免疫学或疫苗组合物。 核苷酸可以在任何合适的载体系统中表达，允许产生多肽。 另外，含有任何或任何组合的核苷酸的载体系统可以用于抗原性，免疫学或疫苗组合物，例如痘病毒载体系统，例如CHV-痘苗或Avipox病毒重组体，以及来自表达的产物， 即gB，gC和gD糖蛋白。 由糖蛋白引起的抗体或含有该核苷酸的载体的表达也是有用的。 还公开并要求保护和使用组合物的方法。 此外，还公开并要求保护特定的金丝雀痘-GV，gC和gD重组体vCP 320，vCP322和vCP294及其制备和使用方法。

    Vectors having enhanced expression, and methods of making and uses thereof
    2.
    发明授权
    Vectors having enhanced expression, and methods of making and uses thereof 失效
    具有增强表达的载体及其制备和使用方法

    公开(公告)号：US5990091A

    公开(公告)日：1999-11-23

    申请号：US816155

    申请日：1997-03-12

    申请人： James Tartaglia ,  William I. Cox ,  Russell Robert Gettig ,  Hector Martinez ,  Enzo Paoletti ,  Steven E. Pincus

    发明人： James Tartaglia ,  William I. Cox ,  Russell Robert Gettig ,  Hector Martinez ,  Enzo Paoletti ,  Steven E. Pincus

    IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K48/00 ,  A61P37/04 ,  C12N15/67 ,  C12N15/85 ,  C12N15/863 ,  C12N15/86

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N15/67 ,  C12N15/85 ,  C12N2710/24043 ,  C12N2710/24143 ,  C12N2830/00 ,  C12N2830/60 ,  C12N2840/00

    摘要： Disclosed and claimed are vectors having enhanced expression and methods for making and using them. Enhancement of expression is from substantially co-temporal expression of at least one first nucleic acid molecule and at least one second nucleic acid molecule. The second nucleic acid molecule encodes a transcription factor or a translation factor or a transcription factor and a translation factor. The contemporaneous expression can be from operably linking the first and second nucleic molecules to a single promoter, or from operably linking the first nucleic acid molecule to a first promoter and the second nucleic molecule to a second promoter wherein the first and second promoters function substantially contemporaneously. Thus, the first and second nucleic acid molecules can be at the same locus in the vector, or at different loci. The second nucleic acid molecule can encode: one transcription factor or more than one transcription factor; or one translation factor or more than one translation factor; or at least one transcription factor and at least one translation factor. The transcription factor can be from vaccinia H4L, D6, A7, G8R, A1L, A2L, H5R, or combinations thereof. The translation factor can be from a K3L open reading frame, an E3L open reading frame, a VAI RNA, an EBER RNA, a sigma 3 open reading frame, a TRBP open reading frame, or combinations thereof. The vector can be a poxvirus such as an attenuated poxvirus, e.g., NYVAC, or ALVAC.

    摘要翻译： 披露和要求保护的是具有增强的表达的载体和用于制备和使用它们的方法。 表达的增强来自至少一种第一核酸分子和至少一种第二核酸分子的基本上同时表达。 第二核酸分子编码转录因子或翻译因子或转录因子和翻译因子。 同时表达可以是将第一和第二核酸分子可操作地连接到单个启动子，或者可操作地将第一核酸分子与第一启动子连接，而将第二核酸分子可操作地连接到第二启动子，其中第一和第二启动子基本同时起作用 。 因此，第一和第二核酸分子可以在载体中的相同基因座处或在不同的基因座处。 第二核酸分子可编码：一种转录因子或多于一种转录因子; 或一个翻译因子或多于一个翻译因子; 或至少一个转录因子和至少一个翻译因子。 转录因子可以来自牛痘H4L，D6，A7，G8R，A1L，A2L，H5R或其组合。 翻译因子可以来自K3L开放阅读框，E3L开放阅读框，VAI RNA，EBER RNA，西格玛3开放阅读框，TRBP开放阅读框或其组合。 载体可以是痘病毒，例如减毒痘病毒，例如NYVAC或ALVAC。

    Malaria recombinant poxviruses
    3.
    发明授权
    Malaria recombinant poxviruses 失效
    疟疾重症痘病毒

    公开(公告)号：US5766597A

    公开(公告)日：1998-06-16

    申请号：US257073

    申请日：1994-06-09

    申请人： Enzo Paoletti ,  Charles de Taisne ,  John A. Tine

    发明人： Enzo Paoletti ,  Charles de Taisne ,  John A. Tine

    IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K39/015 ,  A61K39/275 ,  A61K39/285 ,  C07K14/445 ,  C12N7/01

    CPC分类号： C07K14/445 ,  A61K38/00 ,  A61K39/00

    摘要： What is described is a recombinant poxvirus, such as vaccinia or canarypox virus, containing foreign DNA from Plasmodium such as coding for at least one of CSP, PfSSP2, LSA-1, LSA-1-repeatless, MSA-1, SERA, AMA-1, Pfs25, MSA-1 N-terminal p83 and MSA-1 C-terminal gp42. What is also described is a vaccine containing the recombinant poxvirus for inducing an immunological response in a host animal inoculated with the vaccine. Preferred recombinants have attenuated virulence. In certain embodiments the vaccinia has deleted or disrupted the thymidine kinase gene, the hemorrhagic region, the A type inclusion body region, the host range gene region and, the large subunit, ribonucleotide reductase; and, contains coding sequences for CSP, PfSSP2, LSA-1-repeatless, MSA-1, SERA, AMA-1 and Pfs25. That embodiment is termed NYVAC-Pf7 and is a multicomponent, multistage vaccine since it codes for and expresses sporozoite proteins, liver stage proteins, blood stage proteins and, sexual stage proteins.

    摘要翻译： 描述的是重组痘病毒，例如痘苗或金丝雀痘病毒，其含有来自疟原虫的外来DNA，例如编码CSP，PfSSP2，LSA-1，LSA-1-无重复，MSA-1，SERA，AMA- 1，Pfs25，MSA-1 N端p83和MSA-1 C端gp42。 还描述了含有用于在接种疫苗的宿主动物中诱导免疫应答的重组痘病毒的疫苗。 优选的重组体具有减毒毒力。 在某些实施方案中，痘苗已经缺失或破坏了胸苷激酶基因，出血区域，A型包涵体区域，宿主范围基因区域和大亚基核糖核苷酸还原酶; 并且包含用于CSP，PfSSP2，LSA-1-无重复，MSA-1，SERA，AMA-1和Pfs25的编码序列。 该实施方案称为NYVAC-Pf7，并且是多组分多级疫苗，因为其编码并表达子孢子蛋白，肝阶段蛋白质，血液阶段蛋白质和性阶段蛋白质。

    Nucleotide and amino acid sequences of canine herpesvirus gB and gC
    7.
    发明授权
    Nucleotide and amino acid sequences of canine herpesvirus gB and gC 失效
    犬疱疹病毒gB和gC的核苷酸和氨基酸序列

    公开(公告)号：US5529780A

    公开(公告)日：1996-06-25

    申请号：US220151

    申请日：1994-03-30

    申请人： Enzo Paoletti ,  Keith J. Limbach

    发明人： Enzo Paoletti ,  Keith J. Limbach

    IPC分类号： A61K39/00 ,  C07K14/03 ,  A61K39/245 ,  C12N7/01 ,  C12N15/38 ,  C12P21/02

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/14143 ,  C12N2710/16722

    摘要： Disclosed and claimed are nucleotides for genes encoding the canine herpesvirus (CHV) gB, gC and gD homologues. These genes encode polypeptides of 879, 459 and 345 amino acids, respectively, which are also disclosed. The nucleotides can be expressed in any suitable vector system, allowing for production of the polypeptides. Additionally, the vector system containing any or any combination of the nucleotides can be employed in an antigenic, immunological or vaccine composition, such as a poxvirus vector system, e.g., a CHV-vaccinia or avipox virus recombinant, as can the products from expression, i.e., the gB, gC and gD glycoproteins. Antibodies elicited by the glycoproteins or from expression of the vector containing the nucleotide are also useful. Methods for making and using the composition are also disclosed and claimed.

    摘要翻译： 公开并要求保护的是用于编码犬疱疹病毒（CHV）gB，gC和gD同系物的基因的核苷酸。 这些基因分别编码879,459和345个氨基酸的多肽，其也被公开。 核苷酸可以在任何合适的载体系统中表达，允许产生多肽。 另外，含有任何或任何组合的核苷酸的载体系统可以用于抗原性，免疫学或疫苗组合物，例如痘病毒载体系统，例如CHV-痘苗或Avipox病毒重组体，以及来自表达的产物， 即gB，gC和gD糖蛋白。 由糖蛋白引起的抗体或含有该核苷酸的载体的表达也是有用的。 还公开并要求保护和使用组合物的方法。

    Recombinant poxvirus host range selection system
    10.
    发明授权
    Recombinant poxvirus host range selection system 失效
    重组痘病毒宿主范围选择系统

    公开(公告)号：US5453364A

    公开(公告)日：1995-09-26

    申请号：US102702

    申请日：1993-08-05

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： C12N15/09 ,  A61K39/00 ,  C07K14/03 ,  C07K14/145 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/39 ,  C12N15/863 ,  C12P21/02 ,  C12R1/91 ,  C12N15/86

    CPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12N2710/24143 ,  C12N2760/20122

    摘要： What is described is a modified recombinant virus for expressing a gene product in a host. The modified recombinant virus has host range genes deleted therefrom so that the virus has restricted replication in the host. The modified recombinant virus also contains DNA which codes for and expresses the gene product in the host even with restricted replication of the virus in the host. The modified recombinant virus is used in a method for expressing a gene product in a host or in a cell cultured in vitro, and in a vaccine for inducing an immunological response in a host inoculated with the vaccine. What is also described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conditional lethal mutant (host range) of poxviruses. A deletion/recombinant mutant of the vaccinia virus was generated which is capable of plaquing on primary chick embryo fibroblasts and two monkey cell lines (BSC-40 or VERO) but is defective in replication in the human cell line MRC-5. Insertion of the host range gene into the deletion/recombinant restores the ability for growth on MRC-5 cells. A series of plasmids were constructed which allow for the rapid single-step cloning and expression of any open reading frame when recombined with the deletion/recombinant and scored for growth on MRC-5 cells.

    摘要翻译： 描述的是用于在宿主中表达基因产物的修饰的重组病毒。 修饰的重组病毒具有从其中缺失的宿主范围基因，使得病毒在宿主中具有限制性的复制。 修饰的重组病毒还含有编码并在宿主中表达基因产物的DNA，甚至在宿主中病毒的复制受限。 修饰的重组病毒用于在宿主或体外培养的细胞中表达基因产物的方法，以及用于在接种疫苗的宿主中诱导免疫应答的疫苗。 还描述了在痘病毒，特别是痘苗病毒中克隆和表达开放阅读框的选择系统。 选择系统基于痘病毒的条件致死突变体（宿主范围）。 产生痘苗病毒的缺失/重组突变体，其能够在原代小鸡胚胎成纤维细胞和两种猴细胞系（BSC-40或VERO）上进行染色，但在人细胞系MRC-5中复制缺陷。 将宿主范围基因插入缺失/重组体中恢复MRC-5细胞生长能力。 构建了一系列质粒，当与缺失/重组重组并在MRC-5细胞上进行生长时，允许快速单步克隆和任何开放阅读框的表达。