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    Vectors having enhanced expression, and methods of making and uses thereof
    4.
    发明授权
    Vectors having enhanced expression, and methods of making and uses thereof 失效
    具有增强表达的载体及其制备和使用方法

    公开(公告)号：US6004777A

    公开(公告)日：1999-12-21

    申请号：US815809

    申请日：1997-03-12

    申请人： James Tartaglia ,  Bertram L. Jacobs ,  Scott J. Goebel ,  William I. Cox ,  Russell Robert Gettig ,  Steven E. Pincus ,  Enzo Paoletti

    发明人： James Tartaglia ,  Bertram L. Jacobs ,  Scott J. Goebel ,  William I. Cox ,  Russell Robert Gettig ,  Steven E. Pincus ,  Enzo Paoletti

    IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K48/00 ,  A61P37/04 ,  C12N15/67 ,  C12N15/85 ,  C12P21/02 ,  C12P21/00 ,  C12N15/11 ,  C12N15/63 ,  C12N15/66

    CPC分类号： C12N15/67 ,  C12N15/85

    摘要： Disclosed and claimed are vectors having enhanced expression and methods for making and using them. Enhancement of expression is from substantially co-temporal expression of at least one first nucleic acid molecule and at least one second nucleic acid molecule. The second nucleic acid molecule encodes a translation factor. The contemporaneous expression can be from operably linking the first and second nucleic molecules to a single promoter, or from operably linking the first nucleic acid molecule to a first promoter and the second nucleic molecule to a second promoter wherein the first and second promoters function substantially contemporaneously. Thus, the first and second nucleic acid molecules can be at the same locus in the vector or at different loci. The second nucleic acid molecule can encode encode one translation factor or more than one translation factor. The translation factor can be a K3L open reading frame, an E3L open reading frame, a VAI RNA, an EBER RNA, a sigma 3 open reading frame, a TRBP open reading frame, or combinations thereof. The vector can be a poxvirus such as an attenuated poxvirus; for instance, a NYVAC vaccinia virus or an ALVAC canarypox virus.

    摘要翻译： 披露和要求保护的是具有增强的表达的载体和用于制备和使用它们的方法。 表达的增强来自至少一种第一核酸分子和至少一种第二核酸分子的基本上同时表达。 第二核酸分子编码翻译因子。 同时表达可以是将第一和第二核酸分子可操作地连接到单个启动子，或者可操作地将第一核酸分子与第一启动子连接，而将第二核酸分子可操作地连接到第二启动子，其中第一和第二启动子基本同时起作用 。 因此，第一和第二核酸分子可以在载体中或在不同基因座处于相同位点。 第二核酸分子可编码编码一个翻译因子或多于一个翻译因子。 翻译因子可以是K3L开放阅读框，E3L开放阅读框，VAI RNA，EBER RNA，Sigma 3开放阅读框，TRBP开放阅读框或其组合。 载体可以是痘病毒，如减毒痘病毒; 例如，NYVAC痘苗病毒或ALVAC金丝雀痘病毒。