公开(公告)号：US20170051248A1

公开(公告)日：2017-02-23

申请号：US15345418

申请日：2016-11-07

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Brian Michael Davis ,  Evelina Roxana Loghin ,  Kenneth Roger Conway ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne

IPC: C12N5/00 ,  B29C71/00 ,  B29C37/00 ,  B29C59/02

CPC classification number: C12N5/0068 ,  B29C37/0025 ,  B29C59/02 ,  B29C71/0081 ,  B29K2025/06 ,  B29K2105/256 ,  B29K2883/00 ,  B29L2031/712 ,  C12N2501/999 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  C12N2539/10 ,  Y10T156/1039

Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

Abstract translation: 提供了用于多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体和使用相同载体培养基质细胞的方法。

公开(公告)号：US09037418B2

公开(公告)日：2015-05-19

申请号：US14031951

申请日：2013-09-19

Applicant: General Electric Company

Inventor： Radislav Alexandrovich Potyrailo ,  Cheryl Margaret Surman ,  Andrew Arthur Paul Burns ,  Nandini Nagraj

IPC: G01N27/02 ,  G01N33/487

CPC classification number: G01N27/026 ,  G01N27/02 ,  G01N33/483 ,  G01N33/48792

Abstract: Methods and sensors for selective fluid sensing are provided. Each sensor includes a resonant inductor-capacitor-resistor (LCR) sensor that is coated with a sensing material. In order to collect data, an impedance spectrum is acquired over a relatively narrow frequency range, such as the resonant frequency range of the LCR circuit. A multivariate signature may be calculated from the acquired spectrum to discern the presence of certain fluids and/or fluid mixtures. The presence of fluids is detected by measuring the changes in dielectric, dimensional, resistance, charge transfer, and other changes in the properties of the materials employed by observing the changes in the resonant electronic properties of the circuit. By using a mathematical procedure, such as principal components analysis (PCA) and others, multiple fluids and mixtures can be detected in the presence of one another, even in a high humidity environment or an environment wherein one or more fluids has a substantially higher concentration (e.g. 10×, 1,000,000×) compared to other components in the mixture.

Abstract translation: 提供了选择性流体感测的方法和传感器。 每个传感器包括一个涂有传感材料的谐振电感 - 电容电阻（LCR）传感器。 为了收集数据，在相对窄的频率范围（例如LCR电路的谐振频率范围）上获取阻抗谱。 可以从所获得的光谱计算多变量特征，以辨别某些流体和/或流体混合物的存在。 通过观察电路的谐振电子特性的变化来测量所用材料的介电性质，尺寸，电阻，电荷转移和其它变化的变化来检测流体的存在。 通过使用诸如主成分分析（PCA）等的数学方法，可以在彼此存在的情况下检测多种流体和混合物，即使在高湿度环境或其中一种或多种流体具有显着更高浓度的环境 （例如10×1,000,000×）与混合物中的其他成分相比。

公开(公告)号：US20140356949A1

公开(公告)日：2014-12-04

申请号：US14461860

申请日：2014-08-18

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Evelina Roxana Loghin ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne ,  Scott Michael Miller

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  Y10T156/1039

Abstract: A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.

Abstract translation: 提供了用于诱导多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体的方法，以及使用相同的载体培养诱导的多能干细胞。