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    SYSTEMS AND METHODS FOR ASEPTIC SAMPLING
    3.
    发明申请
    SYSTEMS AND METHODS FOR ASEPTIC SAMPLING 有权
    用于自由采样的系统和方法

    公开(公告)号：US20160122798A1

    公开(公告)日：2016-05-05

    申请号：US14529203

    申请日：2014-10-31

    Applicant: General Electric Company

    Inventor： Chengkun Zhang ,  Kenneth Roger Conway ,  Donald Joseph Buckley ,  Eugene Pauling Boden ,  Weston Blaine Griffin ,  Anshika Bajaj ,  Reginald Donovan Smith

    IPC: C12Q1/24 ,  G01N1/14

    CPC classification number: G01N1/18 ,  C12M23/00 ,  C12M23/40 ,  C12M33/00 ,  C12M33/06 ,  C12M37/02 ,  C12M41/00

    Abstract: A sampling assembly configured to be coupled to a sample source and facilitate aseptic sampling at one or more instances in time is provided. Further, the sampling assembly includes a first conduit having first and second ports, where the first port is configured to be coupled to the sample source. The sampling assembly also includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connector junctions. Also, each of the sub-ports is in fluidic communication with the first conduit. The sampling assembly also includes a plurality of sampling kits and one or more pumping devices. Further, each sampling kit is operatively coupled to a respective sub-port of a corresponding sub-conduit. Moreover, the one or more pumping devices are operatively and aseptically coupled to the second port of the first conduit.

    Abstract translation: 提供了一种采样组件，其被配置为耦合到采样源并且在一个或多个时间上促进无菌采样。 此外，采样组件包括具有第一和第二端口的第一导管，其中第一端口被配置为耦合到采样源。 采样组件还包括具有对应的子端口的多个子管道，其中多个子导管中的每一个在相应的连接器接合处可操作地联接到第一导管。 此外，每个子端口与第一导管流体连通。 采样组件还包括多个采样套件和一个或多个抽吸装置。 此外，每个采样套件可操作地耦合到相应子导管的相应子端口。 此外，一个或多个泵送装置可操作地和无菌地联接到第一管道的第二端口。

    PLURIPOTENT STEM CELL EXPANSION AND PASSAGE USING A STIRRED TANK BIOREACTOR
    4.
    发明申请
    PLURIPOTENT STEM CELL EXPANSION AND PASSAGE USING A STIRRED TANK BIOREACTOR 审中-公开
    使用搅拌罐生物反应器进行超临界干细胞扩增和通气

    公开(公告)号：US20160215257A1

    公开(公告)日：2016-07-28

    申请号：US15075211

    申请日：2016-03-21

    Applicant: General Electric Company

    Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Xiaohua Zhang

    IPC: C12N5/0735

    CPC classification number: C12N5/0606 ,  C12M27/02 ,  C12M33/22

    Abstract: Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems in stirred tank bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.

    Abstract translation: 本文提供了用于扩增和传代包含干细胞和/或分化细胞的细胞聚集体的新颖方法，并且包括在搅拌釜生物反应器中使用封闭系统。 本发明的方法允许具有相关多能性标记和分化潜能的多能干细胞和/或其后代的封闭系统连续通路扩增。

    Systems and methods for aseptic sampling
    5.
    发明授权
    Systems and methods for aseptic sampling 有权

    公开(公告)号：US10465157B2

    公开(公告)日：2019-11-05

    申请号：US14462594

    申请日：2014-08-19

    Applicant: General Electric Company

    Inventor： Chengkun Zhang ,  Kenneth Roger Conway ,  Donald Joseph Buckley ,  Eugene Pauling Boden ,  Weston Blaine Griffin ,  Anshika Bajaj ,  Reginald Donovan Smith ,  Zhipeng Zhang

    IPC: C12M1/00 ,  G01N1/18 ,  B01L3/00 ,  C12M1/32 ,  C12M1/12

    Abstract: A sampling assembly configured to be coupled to a sample source is provided. The sampling assembly is configured to facilitate aseptic sampling at one or more instances in time. The sampling assembly includes a first conduit having a first port and a second port, where the first port is configured to be coupled to the sample source, and where the second port is configured to be hermetically sealed. The sampling assembly further includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connection points, and where each of the sub-ports is in fluidic communication with the first conduit. Moreover, the sampling assembly includes a plurality of sampling kits, where each sampling kit of the plurality of sampling kits is operatively connected to a respective sub-port of a corresponding sub-conduit.

    Semi-automated sampling system for aseptic sampling
    6.
    发明授权
    Semi-automated sampling system for aseptic sampling 有权

    公开(公告)号：US10190950B2

    公开(公告)日：2019-01-29

    申请号：US14529201

    申请日：2014-10-31

    Applicant: General Electric Company

    Inventor： Weston Blaine Griffin ,  Chengkun Zhang ,  Kenneth Roger Conway ,  Reginald Donovan Smith

    IPC: G01N1/14 ,  C12M1/00 ,  C12M1/32 ,  C12M1/12 ,  C12M1/34

    Abstract: A semi-automated sampling assembly configured for aseptic sampling at one or more instances from a sample source having a biological inoculum is provided. The semi-automated sampling assembly includes a sampling conduit, a recovery conduit, one or more sampling kits, and a pumping device. The sampling conduit includes a first port and a second port, where the first port of the sampling conduit is configured to be operatively coupled to the sample source. Further, the recovery conduit includes a first port and a second port, where the first port of the recovery conduit is configured to be operatively coupled to the sample source. Also, the second port of the recovery conduit is operatively coupled to at least a portion of the sampling conduit. Moreover, the one or more sampling kits are operatively coupled to the sampling conduit, and the pumping device is operatively coupled to the sampling conduit.

    Cell carrier, associated methods for making cell carrier and culturing cells using the same
    8.
    发明授权
    Cell carrier, associated methods for making cell carrier and culturing cells using the same 有权
    细胞载体，用于制备细胞载体和使用其的培养细胞的相关方法

    公开(公告)号：US09534206B2

    公开(公告)日：2017-01-03

    申请号：US14461860

    申请日：2014-08-18

    Applicant: General Electric Company

    Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Evelina Roxana Loghin ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne ,  Scott Michael Miller

    IPC: C12N5/074 ,  C12N5/00

    CPC classification number: C12N5/0696 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  Y10T156/1039

    Abstract: A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.

    Abstract translation: 提供了用于诱导多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体的方法，以及使用相同的载体培养诱导的多能干细胞。

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