公开(公告)号：US20160215257A1

公开(公告)日：2016-07-28

申请号：US15075211

申请日：2016-03-21

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Xiaohua Zhang

IPC: C12N5/0735

CPC classification number: C12N5/0606 ,  C12M27/02 ,  C12M33/22

Abstract: Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems in stirred tank bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.

Abstract translation: 本文提供了用于扩增和传代包含干细胞和/或分化细胞的细胞聚集体的新颖方法，并且包括在搅拌釜生物反应器中使用封闭系统。 本发明的方法允许具有相关多能性标记和分化潜能的多能干细胞和/或其后代的封闭系统连续通路扩增。

公开(公告)号：US20170205404A1

公开(公告)日：2017-07-20

申请号：US15366520

申请日：2016-12-01

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Christopher Michael Puleo ,  Ernest William Kovacs ,  Brian Michael Davis

IPC: G01N33/543 ,  G01N33/569

CPC classification number: G01N33/54326 ,  B01L3/00 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/043 ,  G01N33/505 ,  G01N33/54346 ,  G01N33/56972

Abstract: Described are multi-functional beads and methods to capture rare cells directly from low-volume biological samples and perform both functional and genomic assays from those cells. This is accomplished using a multifunctional capture bead that allows co-localization of both the single cell capture element and the molecular assay components. When combined with a digital microfluidic platform this enables encoding and/or barcoding of specific single cells.

公开(公告)号：US20240033092A1

公开(公告)日：2024-02-01

申请号：US17877486

申请日：2022-07-29

Applicant: General Electric Company

Inventor： Gautam Parthasarathy ,  Daniel J. Erno ,  Chitresh Bhushan ,  Cathleen Ann Hoel ,  Sara Kelly Peterson ,  Jessica Susanne Martinez ,  Brian Michael Davis ,  Steven Jude Duclos ,  Fiona Ginty

IPC: A61F2/30

CPC classification number: A61F2/30771 ,  A61F2002/3093 ,  A61F2002/30968 ,  A61F2310/00179 ,  A61F2310/00329 ,  A61F2310/00011 ,  A61F2002/30112

Abstract: The present discussion relates to the design fabrication and use of synthetic scaffold structure for bone growth. In certain implementations the scaffold structures are comprised of a plurality of repeating structures each defined by a local topology. The local topologies are defined at a subset of points in their respective volumes by various parameters including, but not limited to, shape index, curvedness, mean curvature, and Gauss curvature.

公开(公告)号：US09944894B2

公开(公告)日：2018-04-17

申请号：US14956408

申请日：2015-12-02

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Evelina Roxana Loghin ,  Xiaohua Zhang

IPC: C12N5/00 ,  C12M1/00 ,  C12M3/06 ,  C12M1/26 ,  C12M1/33 ,  C12M1/36 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12M29/10 ,  C12M27/16 ,  C12M33/14 ,  C12M33/22 ,  C12M41/48 ,  C12M45/02 ,  C12M47/00 ,  C12M47/02 ,  C12N5/0606 ,  C12N5/0607

Abstract: Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems on rocking platform bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.

公开(公告)号：US09518249B2

公开(公告)日：2016-12-13

申请号：US13839049

申请日：2013-03-15

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Evelina Roxana Loghin ,  Kenneth Roger Conway ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne

IPC: C12N5/0735 ,  C12N5/00

CPC classification number: C12N5/0068 ,  B29C37/0025 ,  B29C59/02 ,  B29C71/0081 ,  B29K2025/06 ,  B29K2105/256 ,  B29K2883/00 ,  B29L2031/712 ,  C12N2501/999 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  C12N2539/10 ,  Y10T156/1039

Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

Abstract translation: 提供了用于多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体和使用相同载体培养基质细胞的方法。

公开(公告)号：US20160340633A1

公开(公告)日：2016-11-24

申请号：US14956408

申请日：2015-12-02

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Evelina Roxana Loghin ,  Xiaohua Zhang

IPC: C12M1/00 ,  C12N5/074 ,  C12N5/0735 ,  C12M3/06 ,  C12M1/36

CPC classification number: C12M29/10 ,  C12M27/16 ,  C12M33/14 ,  C12M33/22 ,  C12M41/48 ,  C12M45/02 ,  C12M47/00 ,  C12M47/02 ,  C12N5/0606 ,  C12N5/0607

Abstract: Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems on rocking platform bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.

Abstract translation: 本文提供了用于扩增和传代包含干细胞和/或分化细胞的细胞聚集体的新颖方法，并且包括在摇摆平台生物反应器上使用封闭系统。 本发明的方法允许具有相关多能性标记和分化潜能的多能干细胞和/或其后代的封闭系统连续通路扩增。

公开(公告)号：US20140186946A1

公开(公告)日：2014-07-03

申请号：US13839049

申请日：2013-03-15

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Evelina Roxana Loghin ,  Kenneth Roger Conway ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne

IPC: C12N5/0735

CPC classification number: C12N5/0068 ,  B29C37/0025 ,  B29C59/02 ,  B29C71/0081 ,  B29K2025/06 ,  B29K2105/256 ,  B29K2883/00 ,  B29L2031/712 ,  C12N2501/999 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  C12N2539/10 ,  Y10T156/1039

Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

Abstract translation: 提供了用于多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体和使用相同载体培养基质细胞的方法。

公开(公告)号：US10100277B2

公开(公告)日：2018-10-16

申请号：US15075211

申请日：2016-03-21

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Xiaohua Zhang

IPC: C12N5/00 ,  C12N5/0735 ,  C12M1/06 ,  C12M1/26

Abstract: Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems in stirred tank bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.

公开(公告)号：US09534206B2

公开(公告)日：2017-01-03

申请号：US14461860

申请日：2014-08-18

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Evelina Roxana Loghin ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne ,  Scott Michael Miller

IPC: C12N5/074 ,  C12N5/00

CPC classification number: C12N5/0696 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  Y10T156/1039

Abstract: A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.

Abstract translation: 提供了用于诱导多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体的方法，以及使用相同的载体培养诱导的多能干细胞。

公开(公告)号：US10350307B2

公开(公告)日：2019-07-16

申请号：US15707074

申请日：2017-09-18

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  John Richard Nelson ,  Wei Gao

IPC: A61K48/00 ,  C12N15/63 ,  C12N15/67 ,  C07H21/02 ,  C07H21/04

Abstract: A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.