公开(公告)号：US20160266064A1

公开(公告)日：2016-09-15

申请号：US14977312

申请日：2015-12-21

Applicant: Genia Technologies, Inc.

Inventor： Kevin Deierling ,  Roger J.A. Chen ,  David J. Fullagar

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487

Abstract: A device for controlling, detecting, and measuring a molecular complex is disclosed. The device comprises a common electrode. The device further comprises a plurality of measurement cells. Each measurement cell includes a cell electrode and an integrator electronically coupled to the cell electrode. The integrator measures the current flowing between the common electrode and the cell electrode. The device further comprises a plurality of analog-to-digital converters, wherein an integrator from the plurality of measurement cells is electrically coupled to one analog-to-digital converter of the plurality of analog-to-digital converters.

公开(公告)号：US20160123923A1

公开(公告)日：2016-05-05

申请号：US14534042

申请日：2014-11-05

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

Abstract translation: 公开了一种在基于纳米孔的测序芯片上输出纳米孔传感器测量的方法。 测量与纳米孔传感器相关的电特性。 处理与纳米孔传感器相关的电特性。 确定电特性和一个或多个先前电特性的总结。 导出了电气特性和一个或多个以前的电气特性的总结。 确定总结包括确定电特性和一个或多个先前的电特性的至少一部分对应于纳米孔传感器处的基本呼叫事件。 总结表示电特性和一个或多个先前电特性的至少一部分。

公开(公告)号：US20150153303A1

公开(公告)日：2015-06-04

申请号：US14558268

申请日：2014-12-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/327

CPC classification number: G01N27/3278 ,  C12Q1/6874 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to: receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.

Abstract translation: 公开了一种用于检测分子复合物的电性能的系统。 该系统包括电耦合到分子复合物的电极，其输出受分子复合物的电特性影响的电信号，其中分子复合物的电特性对电信号的影响的特征在于期望的带宽。 该系统还包括积分放大器电路，其被配置为：从电极接收电信号。 积分放大器电路还被配置为在预定带宽内随时间选择性地放大和积分电信号的一部分，其中至少部分地基于预期带宽来选择预定带宽。

公开(公告)号：US20140221249A1

公开(公告)日：2014-08-07

申请号：US13759701

申请日：2013-02-05

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  David J. Fullager

IPC: G01N27/327

CPC classification number: G01N33/48721

Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

Abstract translation: 公开了一种使用包括在芯片上的多个单元的纳米孔阵列来分析分子的方法。 导致在多个电池的至少一部分中形成纳米孔。 评估纳米孔的第一物理测量。 确定是否使分子与纳米孔相互作用。 导致至少一部分纳米孔与分子相互作用。 评估表明分子性质的纳米孔的第二物理测量。 确定是否使纳米孔被重整，使得细胞可以重新用于与另外的分子相互作用。

公开(公告)号：US20190154623A1

公开(公告)日：2019-05-23

申请号：US16183618

申请日：2018-11-07

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/327 ,  G01N33/487 ,  C12Q1/6874 ,  G01N27/447

CPC classification number: G01N27/3278 ,  C12Q1/6874 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.

公开(公告)号：US20180267013A1

公开(公告)日：2018-09-20

申请号：US15983426

申请日：2018-05-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  David J. Fullagar

IPC: G01N33/487

Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

公开(公告)号：US20180100827A1

公开(公告)日：2018-04-12

申请号：US15819994

申请日：2017-11-21

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/327 ,  G01N33/487

CPC classification number: G01N27/3278 ,  C12Q1/6874 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.

公开(公告)号：US20170254797A1

公开(公告)日：2017-09-07

申请号：US15462483

申请日：2017-03-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  David J. Fullagar

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

公开(公告)号：US20160178554A1

公开(公告)日：2016-06-23

申请号：US14971667

申请日：2015-12-16

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/22 ,  C12Q1/68 ,  G01N33/487 ,  G01N27/447

CPC classification number: G01N27/228 ,  C12Q1/6869 ,  G01N27/3273 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

Abstract translation: 公开了分析分子的方法。 使用由复位信号控制的开关，电压源选择性地连接到电容器或从电容器断开。 当电压源连接到电容器时，电容被存储在电容器中。 当电压源与电容器断开时，电容器通过膜中的纳米孔排出。 确定复位信号的占空比，使得电压源和电容器连接至复位信号周期的至少十分之一，并且对于复位信号周期的剩余部分断开，使得跨越纳米孔的电压 在维持连接的复位信号周期的部分期间保持在比不保持连接的复位信号周期的剩余部分期间更高的电平。

公开(公告)号：US20150107996A1

公开(公告)日：2015-04-23

申请号：US14056795

申请日：2013-10-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.

Abstract translation: 公开了鉴定分子的方法。 通过在第一周期期间将一个第一电压信号施加到一对电极上，将一个分子吸引到纳米孔，其中第一电压信号引起通过纳米孔的第一离子电流，其指示分子的一部分的性质接近于 纳米孔。 在第二时段期间，通过在所述一对电极施加第二电压信号，从所述纳米孔释放所述分子，其中所述第二电压信号引起通过所述纳米孔的第二离子电流。 至少部分地基于通过包含第一离子电流和第二离子电流的纳米孔的净离子电流确定第一周期和第二周期。