公开(公告)号：US10273536B2

公开(公告)日：2019-04-30

申请号：US15066117

申请日：2016-03-10

Applicant: Genia Technologies, Inc.

Inventor： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech ,  Hui Tian ,  Robert Yuan ,  John Foster ,  Roger Chen

IPC: G01N27/327 ,  C12Q1/6869 ,  C12M1/34 ,  G01N33/487 ,  G01N27/447

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

公开(公告)号：US10102338B2

公开(公告)日：2018-10-16

申请号：US14864408

申请日：2015-09-24

Applicant: Genia Technologies, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  Bill Maney ,  Jayalakshmi Rajaraman

IPC: G01N33/48 ,  G01N33/50 ,  G06F19/22 ,  G01N33/487

Abstract: A system includes a plurality of nanopore cells. Data corresponding to nanopore states of the plurality of nanopore cells is received. The data is analyzed to determine a compressed output size of the data given at least one compression technique. It is determined whether the compressed output size exceeds a data budget. In the event it is determined that the compressed output size exceeds the data budget, the data is modified. The modified data is outputted.

公开(公告)号：US10060903B2

公开(公告)日：2018-08-28

申请号：US14534042

申请日：2014-11-05

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/447 ,  G01N27/22 ,  G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US20180136159A1

公开(公告)日：2018-05-17

申请号：US15820032

申请日：2017-11-21

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  J. William Maney

IPC: G01N27/22 ,  G01N33/487 ,  C12Q1/6869 ,  G01N27/447

CPC classification number: G01N27/228 ,  C12Q1/6869 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

公开(公告)号：US20170199150A1

公开(公告)日：2017-07-13

申请号：US15371106

申请日：2016-12-06

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  J. William Maney, Jr.

IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/68

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

公开(公告)号：US20170091381A1

公开(公告)日：2017-03-30

申请号：US14864408

申请日：2015-09-24

Applicant: Genia Technologies, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  Bill Maney ,  Jayalakshmi Rajaraman

IPC: G06F19/22

CPC classification number: G06F19/22 ,  G01N33/48721

Abstract: A system includes a plurality of nanopore cells. Data corresponding to nanopore states of the plurality of nanopore cells is received. The data is analyzed to determine a compressed output size of the data given at least one compression technique. It is determined whether the compressed output size exceeds a data budget. In the event it is determined that the compressed output size exceeds the data budget, the data is modified. The modified data is outputted.

公开(公告)号：US20160216233A1

公开(公告)日：2016-07-28

申请号：US14606632

申请日：2015-01-27

Applicant: Genia Technologies, Inc.

Inventor： Jennifer Hovis ,  Hui Tian ,  Roger J.A. Chen

IPC: G01N27/447 ,  G01N33/487

CPC classification number: G01N33/48721

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

Abstract translation: 公开了一种纳米孔测序装置。 纳米孔测序装置包括工作电极。 还包括电介质层，其中电介质层的一部分水平地邻近工作电极设置，并且介电层的一部分设置在工作电极的一部分上方并覆盖其中，并且其中介电层形成具有 在工作电极的未覆盖部分上方的开口。 工作电极的基面面积大于工作电极的未覆盖部分上方开口的基面面积。

公开(公告)号：US20160178577A1

公开(公告)日：2016-06-23

申请号：US14577511

申请日：2014-12-19

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/447 ,  C12Q1/68 ,  B01L3/00

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

Abstract translation: 公开了分析纳米孔中分子的方法。 通过将纳米孔耦合到电压源，跨越插入膜中的纳米孔施加电压。 纳米孔与电压源分离。 在解耦之后，确定纳米孔上电压的衰减速率。 基于确定的纳米孔上的电压衰减速率，纳米孔中的分子与其它可能的分子区分开来。