摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所述的核苷酸序列,所述核苷酸序列如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-30959,B-30960,B-30943或B-50048,以及其变体和片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所述的核苷酸序列,所述核苷酸序列如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-30959,B-30960,B-30943或B-50048,以及其变体和片段。
摘要:
Four new strains of Bacillus thuringiensis are disclosed which are active against Coleoptera. The novel strains are identified through PCR technology using mixtures of carefully selected oligonucleotide primers that aid in the prediction of insect toxicity profile for the proteins produced by the strains.
摘要:
Four new strains of Bacillus thuringiensis are disclosed which are active against Coleoptera. The novel strains are identified through PCR technology using mixtures of carefully selected oligonucleotide primers that aid in the prediction of insect toxicity profile for the proteins produced by the strains.
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:3和5所示的氨基酸序列的核苷酸序列和SEQ ID NO:1,2和4所示的核苷酸序列的分离的核酸分子,以及 作为其变体和片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:2所示的氨基酸序列的核苷酸序列或SEQ ID NO:1所示的核苷酸序列的分离的核酸分子及其变体。
摘要:
Compositions and methods for rapid and highly efficient characterization of genetic diversity in organisms are provided. The methods involve rapid sequencing and characterization of extrachromosomal DNA, particularly plasmids, to identify useful nucleotide sequences. The method involves generating a library of extrachromosomal DNA clones, sequencing a portion of the clones, comparing the sequences against a database of existing DNA sequences, using an algorithm to select novel nucleotide sequences based on the presence or absence of the sequence in a database, and identification of at least one novel nucleotide sequence. The DNA sequence can also be translated in all six frames and the resulting amino acid sequences compared against a database of protein sequences. Organisms of particular interest include, but are not limited to bacteria, fungi, algae, and the like. Compositions comprise a mini-cosmid vector comprising a stuffer fragment and at least one cos site.
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO:3,5和7所示氨基酸序列的核苷酸序列,以及SEQ ID NO: 和6,以及其变体和片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO:1或3所示的核苷酸序列的分离的核酸分子及其变体 。