HIGHLY ALKALINE TEXTILE WASHING AGENT COMPRISING PROTEASE

    公开(公告)号:US20230174904A1

    公开(公告)日:2023-06-08

    申请号:US17920407

    申请日:2021-03-23

    摘要: The present invention relates to a textile washing agent, in particular a liquid textile washing agent, comprising a) at least one protease, wherein the protease has proteolytic activity and comprises an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1, has (i) at the positions that correspond to positions 9, 130, 133, 144, 217, 252 and 271, amino acid substitutions, in particular the amino acid substitutions 9T, 130D/V, 133A, 144K, 217M, 252T and 271E, and (ii) at at least one, preferably at least two, of the positions that correspond to positions 6, 89, 131, 166, 189, 211 or 224, at least one additional amino acid substitution, in particular selected from 6W/F, 89A/G, 131H/Y/F, 166M/L/I, 189T/L/I, 211N/Q and 224A/G, more preferably selected from 6W, 89A, 131H, 166M, 189T, 211N and 224A, and b) at least one washing agent ingredient, preferably in an amount of from 0.01 to 99.9 wt.%, wherein the textile washing agent has a pH of from approximately 9 to approximately 12, measured in a 1 wt.% solution in deionized water at 20° C. The present invention also relates to corresponding methods for cleaning textiles, in which methods agents according to the invention are used, and to uses of agents according to the invention for removing protease-sensitive stains from textiles.

    PERFORMANCE-ENHANCED PROTEASE VARIANTS VII
    27.
    发明公开

    公开(公告)号:US20230159907A1

    公开(公告)日:2023-05-25

    申请号:US17908554

    申请日:2021-02-25

    IPC分类号: C12N9/54 C11D3/386

    CPC分类号: C12N9/54 C11D3/386

    摘要: Proteases may include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length. The proteases may further have, in each case based on the numbering according to SEQ ID NO:1, amino acid substitutions 9T, 130D/V, 133A, 144K, 217M, 224A, 252T and 271E at the positions corresponding to positions 9, 130, 133, 144, 217, 224, 252 and 271. The proteases may further include at least one further amino acid substitution at at least one of the positions corresponding to positions 89, 131 and 189, and to the production and use thereof. Such proteases exhibit very good cleaning performance.