公开(公告)号：US20100003683A1

公开(公告)日：2010-01-07

申请号：US12373517

申请日：2007-07-05

申请人： Emad Sarofim ,  Olivier Elsenhans ,  Martin Kopp ,  Hans-Peter Wahl

发明人： Emad Sarofim ,  Olivier Elsenhans ,  Martin Kopp ,  Hans-Peter Wahl

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M1/34

CPC分类号： B01L3/502715 ,  B01L3/502707 ,  B01L2200/10 ,  B01L2300/0809

摘要： The invention is directed to a disposable sample holding and processing device (1) dimensioned for being operated in a nucleic acid amplification apparatus for analyzing a liquid sample containing a nucleic acid by a nucleic acid amplification technique. The device (1) comprises chambers (15, 16) and channels (44) designed to perform the steps of capturing, amplification and detection of the nucleic acid within the device (1), and optionally also a lysis chamber (3). The binding chamber (15) contains a solid phase (14) for immobilization of a component of the sample to be analyzed, and the amplification chamber (16) is connected to the binding chamber (15) by a fluid channel (44). The device (1) comprises a rigid body (42) and at least one channel (44), the binding chamber (15) and the amplification chamber (16) are situated on side-surfaces (50) of the body (42), each of those side-surfaces (50), on which a channel (44), the binding chamber (15) or the amplification chamber (16) is situated, is covered by at least one wall (41) and these side-surfaces (50) are substantially vertical planes when the device (1) is operated in the nucleic acid amplification apparatus.

摘要翻译： 本发明涉及一种一次性样品保持和处理装置（1），其尺寸适于在用于通过核酸扩增技术分析含有核酸的液体样品的核酸扩增装置中操作。 设备（1）包括设计成执行在装置（1）内捕获，扩增和检测核酸以及任选还有裂解室（3）的步骤的室（15,16）和通道（44）。 装订室（15）包含用于固定待分析样品的组分的固相（14），并且扩散室（16）通过流体通道（44）连接到装订室（15）。 装置（1）包括刚体（42）和至少一个通道（44），装订室（15）和放大室（16）位于主体（42）的侧表面（50）上， 其中通道（44），装订室（15）或放大室（16）所在的那些侧表面（50）中的每一个被至少一个壁（41）和这些侧表面 50）在装置（1）在核酸扩增装置中操作时是基本垂直的平面。

公开(公告)号：US20080056947A1

公开(公告)日：2008-03-06

申请号：US11828959

申请日：2007-07-26

申请人： Michael Glauser ,  Hans-Peter Wahl ,  Irio Calasso ,  Emad Sarofim

发明人： Michael Glauser ,  Hans-Peter Wahl ,  Irio Calasso ,  Emad Sarofim

IPC分类号： B01J19/00 ,  B05D1/02 ,  B05D3/00 ,  B05D3/04 ,  B05D7/22

CPC分类号： A61B5/1411 ,  A61B5/14532 ,  A61B5/150022 ,  A61B5/150282 ,  A61B5/150419 ,  A61B5/150435 ,  A61B5/150503 ,  A61B5/15105 ,  A61B5/15142 ,  A61B2562/0295 ,  B01L3/502707 ,  B01L2300/0825 ,  B01L2300/161 ,  B01L2300/163 ,  B01L2400/0406 ,  Y10T436/2575

摘要： A microfluidic system having a microchannel for the capillary transport of a liquid, in particular a body fluid for analytical purposes. The microchannel is provided with a surface coating comprising at least one hydrophilic substance selected from the group consisting of polyacrylic acid, polyacrylate, dextran sulfate and chondroitin sulfate. The invention also concerns a coating method that is suitable for this.

摘要翻译： 一种微流体系统，具有用于毛细管理液体的微通道，特别是用于分析目的的体液。 微通道设置有包含至少一种选自聚丙烯酸，聚丙烯酸酯，硫酸葡聚糖和硫酸软骨素的亲水性物质的表面涂层。 本发明还涉及适合于此的涂布方法。