公开(公告)号：US08814808B2

公开(公告)日：2014-08-26

申请号：US12879500

申请日：2010-09-10

申请人： Irio Guiseppe Calasso ,  Patrick Griss ,  Emad Sarofim ,  Rainer Jaeggi ,  Uwe Kraemer ,  Dave Hasker ,  Volker Zimmer ,  Wilifried Schmid ,  Otto Fuerst ,  Hans List ,  Hans-Peter Haar ,  Theo Arnitz ,  Steven N. Roe

发明人： Irio Guiseppe Calasso ,  Patrick Griss ,  Emad Sarofim ,  Rainer Jaeggi ,  Uwe Kraemer ,  Dave Hasker ,  Volker Zimmer ,  Wilifried Schmid ,  Otto Fuerst ,  Hans List ,  Hans-Peter Haar ,  Theo Arnitz ,  Steven N. Roe

IPC分类号： A61B5/151 ,  A61B5/15 ,  A61B5/145

CPC分类号： A61B5/1411 ,  A61B5/14532 ,  A61B5/1486 ,  A61B5/150022 ,  A61B5/150068 ,  A61B5/150282 ,  A61B5/150358 ,  A61B5/150396 ,  A61B5/150419 ,  A61B5/150427 ,  A61B5/15045 ,  A61B5/150503 ,  A61B5/15151 ,  A61B5/15163

摘要： Body fluid sampling device comprising a skin-piercing element having a collection zone for receiving body fluid. In one embodiment, the collection zone includes a plurality of holes, e.g., 3 or more holes, or 50 to 100 holes. The hole size may be small, e.g., having a diameter of 0.01 to 0.5 mm. The holes may or may not extend through the lancet. In some embodiments, the holes have a depth of 50 to 500 μm. In other embodiments, the collection zone is porous, or is a roughened area. The collection zone can take up a very small volume of body fluid, e.g., 3 to 10 nL, in a very short time period, e.g., less than 0.5 seconds. In other embodiments, the device further comprises a fluid receiving means spaced apart from the collection zone so that body fluid in the collection zone will not contact the fluid receiving means initially.

摘要翻译： 体液取样装置包括具有用于接收体液的收集区的皮肤刺穿元件。 在一个实施例中，收集区包括多个孔，例如3个或更多个孔，或50至100个孔。 孔尺寸可以较小，例如具有0.01至0.5mm的直径。 这些孔可以延伸穿过刺血针也可能不延伸穿过刺血针。 在一些实施例中，孔的深度为50至500μm。 在其它实施方案中，收集区是多孔的，或者是粗糙区域。 收集区可以在非常短的时间段（例如小于0.5秒）内占据非常小体积的体液，例如3至10nL。 在其他实施例中，该装置还包括与收集区间隔开的流体接收装置，使得收集区中的体液最初不会接触流体接收装置。

公开(公告)号：US08580567B2

公开(公告)日：2013-11-12

申请号：US12903492

申请日：2010-10-13

申请人： Emad Sarofim ,  Edwin Oosterbroek ,  Claudio Cherubini ,  Nenad Milicevic

发明人： Emad Sarofim ,  Edwin Oosterbroek ,  Claudio Cherubini ,  Nenad Milicevic

IPC分类号： G01N21/07

CPC分类号： G01N35/00029 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0677 ,  G01N21/07 ,  G01N2021/0325 ,  G01N2035/00099 ,  G01N2035/00495

摘要： A method for the automated analysis of liquid samples using at least one microfluidic structure is disclosed as well as microfluidic structures, a device having at least one of the microfluidic structures, a kit and a system including such microfluidic device. In one embodiment, the method may comprise: transferring a sample into a first fluid reservoir which is in fluid communication with a second fluid reservoir by a flow channel; spinning the microfluidic structure so as to transport the sample into one or more dead-end recesses or chambers; transferring a control fluid into the structure which generates a barrier to flow and diffusion of the sample contained in the one or more dead-end recesses or chambers; and analyzing the sample contained in the one or more dead-end recesses or chambers.

摘要翻译： 公开了使用至少一个微流体结构自动分析液体样品的方法，以及微流体结构，具有至少一个微流体结构的装置，试剂盒和包括这种微流体装置的系统。 在一个实施例中，该方法可以包括：将样品转移到通过流动通道与第二流体储存器流体连通的第一流体储存器中; 旋转微流体结构以将样品输送到一个或多个死端凹部或腔室中; 将控制流体转移到所述结构中，所述结构产生包含在所述一个或多个死端凹部或腔室中的样品的流动和扩散的阻挡层; 并分析包含在一个或多个死端凹部或腔室中的样品。

公开(公告)号：US20120295249A1

公开(公告)日：2012-11-22

申请号：US13295504

申请日：2011-11-14

申请人： Claudio Cherubini ,  Roger Iten ,  Emad Sarofim ,  Kurt Schildknecht

发明人： Claudio Cherubini ,  Roger Iten ,  Emad Sarofim ,  Kurt Schildknecht

IPC分类号： G01N21/75 ,  C12Q1/70 ,  C12Q1/06 ,  C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/022 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1805 ,  C12Q1/686 ,  G01N21/0332 ,  G01N21/253 ,  G01N21/645 ,  G01N21/6452 ,  G01N35/028 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2035/00346 ,  G01N2035/00376 ,  G01N2201/0627

摘要： An instrument and a method for the automated thermal treatment of liquid samples are disclosed. An inter-distance between a temperature-controlled receptacle for loading with a plurality of vessels for containing the samples and end portions of optical fibers can be varied, wherein the receptacle is configured to form a thermal communication with the loaded vessels and wherein the optical fibers have first and second end portions. The first end portion and the second end portion of each optical fiber is fixed with respect to each other for transmitting light, wherein the variation of the inter-distance allows the vessels to be loaded to or unloaded from the receptacle and to enable detection of light from the samples contained in the one or more receptacle-loaded vessels.

摘要翻译： 公开了用于液体样品的自动热处理的仪器和方法。 用于装载用于容纳样品和光纤的端部的多个容器的温度控制容器之间的距离可以变化，其中容器被配置为与加载的容器形成热连通，并且其中光纤 具有第一和第二端部。 每个光纤的第一端部和第二端部相对于彼此固定以透射光，其中间距的变化允许容器被装载或从插座卸载并且能够检测光 从包含在一个或多个容器加载的容器中的样品。

公开(公告)号：US08162854B2

公开(公告)日：2012-04-24

申请号：US11470021

申请日：2006-09-05

申请人： Irio G. Calasso ,  Patrick Griss ,  Rainer Jaeggi ,  Emad Sarofim

发明人： Irio G. Calasso ,  Patrick Griss ,  Rainer Jaeggi ,  Emad Sarofim

IPC分类号： B65D81/00

CPC分类号： A61B5/14532 ,  A61B5/1411 ,  A61B5/1486 ,  A61B5/150022 ,  A61B5/150251 ,  A61B5/150297 ,  A61B5/150358 ,  A61B5/150419 ,  A61B5/150435 ,  A61B5/150503 ,  A61B5/15107 ,  A61B5/15117

摘要： Body fluid sampling device comprising a skin-piercing element (10) having a fluid pathway (11) for receiving body fluid, at least a portion of the fluid pathway is open to the environment and further comprising a fluid receiving element (40) being spaced from the fluid pathway so that fluid in the pathway will not contact the fluid receiving element initially. The fluid receiving element may have a test zone (45) for performing an analytical reaction. Fluid from the channel is contacted with the fluid receiving element either by bringing the fluid receiving element and the fluid into mechanical contact or by electrically transporting fluid from the channel onto the fluid receiving element.

摘要翻译： 体液取样装置包括具有用于接收体液的流体通道（11）的皮肤刺穿元件（10），所述流体通道的至少一部分对环境开放，并且还包括间隔开的流体接收元件（40） 从流体通路使得通道中的流体最初不会接触流体接收元件。 流体接收元件可以具有用于进行分析反应的测试区（45）。 通过将流体接收元件和流体机械接触或将流体从通道电输送到流体接收元件上，来自通道的流体与流体接收元件接触。

公开(公告)号：US08062884B2

公开(公告)日：2011-11-22

申请号：US12373518

申请日：2007-07-05

申请人： Emad Sarofim

发明人： Emad Sarofim

IPC分类号： C12M1/34

CPC分类号： B01L3/502715 ,  B01L3/0275 ,  B01L3/502723 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487

摘要： The invention refers to a handling kit for analyzing a liquid sample, especially by nucleic acid amplification, comprising a disposable sample holding and processing device (1) dimensioned for use in an apparatus for analyzing a liquid sample, and a sample transfer tip (12) for transferring liquid into the disposable device (1), the disposable device (1) having a sample preparation chamber (10) which has an outlet (9) and an insertion opening (16) which is adapted to receive the sample transfer tip (12), the insertion opening and the sample transfer tip (12) being dimensioned in such a way that inserting the sample transfer tip (12) into an insertion position in the sample preparation chamber (10) causes a tight seal between an outer wall (40) of the sample transfer tip (12) and an inner wall (41) of the sample preparation chamber (10), the disposable device (1) having a vent (31) for venting the sample preparation chamber (10).

摘要翻译： 本发明涉及用于分析液体样品的处理工具，特别是通过核酸扩增来分析液体样品，其包括一次性样品保持和处理装置（1），其尺寸用于分析液体样品的装置和样品转移尖端（12） 用于将液体输送到一次性装置（1）中，一次性装置（1）具有样品制备室（10），其具有出口（9）和插入开口（16），其适于接收样品转移尖端 ），所述插入开口和所述样本转移尖端（12）的尺寸被设计成将所述样品转移尖端（12）插入样品制备室（10）中的插入位置，使得在外壁（40） ）和所述样品制备室（10）的内壁（41）的一次性装置（1）具有用于排出样品制备室（10）的通气孔（31）。

公开(公告)号：US20110127292A1

公开(公告)日：2011-06-02

申请号：US12786709

申请日：2010-05-25

申请人： Emad Sarofim ,  Martin Kopp ,  Claudio Cherubini

发明人： Emad Sarofim ,  Martin Kopp ,  Claudio Cherubini

IPC分类号： B67D7/06 ,  B01L3/02 ,  B01L9/00

CPC分类号： G01N35/1083 ,  B01L9/52 ,  B01L9/523 ,  B01L2300/0829 ,  G01N35/1065

摘要： A system for dispensing one or more fluids into wells of a target multi-well plate, a holder and a method thereof are disclosed. The system provides the holder which holds the multi-well plate in a predefined holding position, the plate having a well region provided with wells for accommodating the fluids and an edge region surrounding the well region. The holder includes: a contact area which contacts the edge region to form a sealing zone which air-tightly seals a void formed between the holder and the plate; a supporting face which supports the well region in a planar condition; and a duct which connects to a pump to generate a negative pressure in the void so as to draw the well region onto the supporting face.

摘要翻译： 公开了一种用于将一种或多种流体分配到目标多孔板的孔中的系统，保持器及其方法。 该系统提供将多孔板保持在预定的保持位置的保持器，该板具有设置有用于容纳流体的井的井区域和围绕井区域的边缘区域。 保持器包括：接触区域，其接触边缘区域以形成气密密封形成在保持器和板之间的空隙的密封区域; 在平坦状态下支撑井区的支撑面; 以及连接到泵以在空隙中产生负压以将阱区域拉到支撑面上的管道。

公开(公告)号：US20100064781A1

公开(公告)日：2010-03-18

申请号：US12556925

申请日：2009-09-10

申请人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

发明人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

IPC分类号： G01N21/00 ,  B01L3/00 ,  G01N1/44

CPC分类号： B01L3/50851 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858

摘要： Systems, methods and multi-well plates comprising an array of wells for thermal treatment of chemical or biological samples are disclosed. Each of the wells comprises a bottom opening, an upper opening, inner side walls extending from the bottom opening to the upper opening, a protrusion extending from the inner side walls into the well with a first cross-sectional area and located at a height from the bottom opening which is smaller than the height from the upper opening. A sample chamber with a second cross-sectional area is formed between the bottom opening and the protrusion. An upper chamber with a third cross-sectional area is formed between the protrusion and the upper opening, and in which the first cross-sectional area is smaller than the third cross-sectional area and smaller than or equal to the second cross-sectional area.

摘要翻译： 公开了包括用于化学或生物样品的热处理的孔阵列的系统，方法和多孔板。 每个孔包括底部开口，上部开口，从底部开口延伸到上部开口的内侧壁，从内侧壁延伸进入井的突起，其具有第一横截面积并且位于与 底部开口小于从上部开口的高度。 具有第二横截面积的样品室形成在底部开口和突起之间。 在所述突起与所述上部开口之间形成具有第三横截面积的上部腔室，其中，所述第一横截面积小于所述第三横截面积，并且小于或等于所述第二横截面积 。

公开(公告)号：US20090317899A1

公开(公告)日：2009-12-24

申请号：US12373518

申请日：2007-07-05

申请人： Emad Sarofim

发明人： Emad Sarofim

IPC分类号： C12M1/34

CPC分类号： B01L3/502715 ,  B01L3/0275 ,  B01L3/502723 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487

摘要： The invention refers to a handling kit for analyzing a liquid sample, especially by nucleic acid amplification, comprising a disposable sample holding and processing device (1) dimensioned for use in an apparatus for analyzing a liquid sample, and a sample transfer tip (12) for transferring liquid into the disposable device (1), the disposable device (1) having a sample preparation chamber (10) which has an outlet (9) and an insertion opening (16) which is adapted to receive the sample transfer tip (12), the insertion opening and the sample transfer tip (12) being dimensioned in such a way that inserting the sample transfer tip (12) into an insertion position in the sample preparation chamber (10) causes a tight seal between an outer wall (40) of the sample transfer tip (12) and an inner wall (41) of the sample preparation chamber (10), the disposable device (1) having a vent (31) for venting the sample preparation chamber (10).

摘要翻译： 本发明涉及用于分析液体样品的处理工具，特别是通过核酸扩增来分析液体样品，包括一次性样品保持和处理装置（1），其尺寸用于分析液体样品的装置和样品转移尖端（12） 用于将液体输送到一次性装置（1）中，一次性装置（1）具有样品制备室（10），其具有出口（9）和插入开口（16），其适于接收样品转移尖端 ），所述插入开口和所述样本转移尖端（12）的尺寸被设计成将所述样品转移尖端（12）插入样品制备室（10）中的插入位置，使得外壁（40） ）和所述样品制备室（10）的内壁（41）的一次性装置（1）具有用于排出样品制备室（10）的通气孔（31）。

公开(公告)号：US20080026430A1

公开(公告)日：2008-01-31

申请号：US11777164

申请日：2007-07-12

申请人： Emad Sarofim ,  Goran Savatic

发明人： Emad Sarofim ,  Goran Savatic

IPC分类号： C12P19/34 ,  B01J19/00 ,  F24J3/00 ,  F28D21/00

CPC分类号： B01L7/52 ,  B01L7/00 ,  B01L2300/1827 ,  B01L2300/1883 ,  Y02P20/582

摘要： An instrument and method is provided that conveniently allows rapid amplification of nucleic acids.

摘要翻译： 提供了方便地允许核酸快速扩增的仪器和方法。

公开(公告)号：US20060002827A1

公开(公告)日：2006-01-05

申请号：US11168695

申请日：2005-06-28

申请人： Mario Curcio ,  Emad Sarofim

发明人： Mario Curcio ,  Emad Sarofim

IPC分类号： B01L11/00

CPC分类号： B01L3/563 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/0838

摘要： A device and method for interconnecting at least one reservoir for holding at least one liquid with at least one area in or on a test element to be supplied and/or brought into contact with the liquid is provided. In accordance with one embodiment, the reservoir and the area to be supplied and/or brought into contact with the liquid are integrated on the same test element or are arranged on separate embodiments. The device comprises at least one capillary-like tube or conduit comprising an inlet and an outlet at opposite ends thereof, which tube or conduit is at least along a section at least nearly rigid.

摘要翻译： 提供一种用于互连至少一个储存器的装置和方法，用于将至少一种液体与待供应和/或与液体接触的测试元件中或之上的至少一个区域进行保持。 根据一个实施例，储存器和要供应和/或与液体接触的区域被集成在相同的测试元件上或者被布置在分开的实施例上。 该装置包括至少一个毛细管状管或导管，其包括在其相对端部处的入口和出口，该管或导管至少沿着至少几乎刚性的部分。