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公开(公告)号：US07473892B2
公开(公告)日：2009-01-06
申请号：US10849517
申请日：2004-05-20
申请人： Akihiro Sano , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari , Kenko Uchida , Toshiyuki Yokosuka
发明人： Akihiro Sano , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari , Kenko Uchida , Toshiyuki Yokosuka
CPC分类号： G01N33/6851 , G01N33/6848 , H01J49/0031 , H01J49/0036 , H01J49/004
摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.
摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和来自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。
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公开(公告)号：US07435949B2
公开(公告)日：2008-10-14
申请号：US11439949
申请日：2006-05-25
CPC分类号： H01J49/02 , H01J49/004
摘要： A tandem analysis system is provided for ionizing a substance, performing mass spectrometric analysis of various ion types generated, selecting and dissociating an ion type, the ion type having a specific mass-to-charge ratio, and thereby, repeating mass spectrometric analysis measurement on the ion of the ion type over n-th stages. A processing judges control content for the analysis next to MSn (the n-th stage mass spectrometric analysis) within a predetermined time, based on ion intensity being represented by an ion peak with respect to the mass-to-charge ratio of each ion in the MSn result. An ion detection unit judges isotope-peak from the measured ionized data. Assuming that the MS1 count number of a parent-ion peptide measured during a certain constant time-interval is I, a data processing unit makes the MS2 integration number-of-times or analysis time of the peptide proportional to 1/I.
摘要翻译： 提供用于电离物质的串联分析系统，对所生成的各种离子类型进行质谱分析，选择和离解离子类型，具有特定质荷比的离子类型，从而重复质谱分析测量 在第n级的离子型离子。 基于离子强度由离子峰相对于质量分数表示，A处理在预定时间内判断用于分析MS（次级质谱分析）旁边的分析的控制内容， 在MS＆lt; n＆gt;结果中的每个离子的电荷比。 离子检测单元根据测得的电离数据判断同位素峰。 假设在一定的时间间隔期间测量的亲代离子肽的MS 1的计数值为I，则数据处理单元使MS <2> 时间或肽的分析时间与1 / I成比例。
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公开(公告)号：US07332713B2
公开(公告)日：2008-02-19
申请号：US11210965
申请日：2005-08-25
IPC分类号： B01D59/44
CPC分类号： H01J49/04 , G01N30/24 , G01N30/467 , G01N30/7206 , G01N30/724 , G01N30/8658 , H01J49/0027
摘要： In a method for mass spectrometry, a plurality of juxtaposed chromatography apparatus connected to a mass spectrometer start eluting at a predetermined time difference and the following mass spectrometer conducts mass spectrometry. A chromatogram in a preceding chromatography apparatus is analyzed on real time base and results of the analysis are used on real time base to change an elusion condition of a succeeding chromatography apparatus. A mass spectrometric system suitable for carrying out the method is also provided.
摘要翻译： 在质谱法中，与质谱仪连接的多个并置色谱装置开始以预定的时间差洗脱，随后的质谱仪进行质谱分析。 在实时基础上分析先前色谱装置中的色谱图，并将实时分析结果用于改变后续色谱装置的脱色条件。 还提供了适用于实施该方法的质谱系统。
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24.发明授权公开(公告)号：US07158893B2
公开(公告)日：2007-01-02
申请号：US10993495
申请日：2004-11-22
申请人： Kiyomi Yoshinari , Kinya Kobayashi , Lee Chahn
发明人： Kiyomi Yoshinari , Kinya Kobayashi , Lee Chahn
CPC分类号： H01J49/0036 , G06F19/16 , G06F19/703
摘要： A main object is to cope with an unknown structure substance thereby to identify the structure of a parent ion highly precisely and to derive a supposed structure. A method for analyzing mass spectrometric data is disclosed, which: acquires mass spectrometric data on an ionized sample and dissociated ions dissociated from the sample as a parent ion; derives dissociated ion candidates by analyzing the molecular orbits on the candidates of the structures of the parent ion; and displays the analytical results of the parent ion candidates and the dissociated ion candidates and compares the data of the dissociated ion candidates and the data of dissociated ions actually measured, to evaluate the structures of the parent ion candidates.
摘要翻译： 主要目的是应对未知的结构物质，从而高度精确地鉴定母体离子的结构，并得出假定的结构。 公开了一种用于分析质谱数据的方法，其获取离子化样品和离开样品离解的离子离子作为母离子的质谱数据; 通过分析母体离子结构的候选物上的分子轨道衍生离解离子候选物; 并显示母体离子候选物和解离离子候选物的分析结果，并比较实际测量的离解离子候选物和离解离子数据的数据，以评估母离子候选物的结构。
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公开(公告)号：US20060169889A1
公开(公告)日：2006-08-03
申请号：US11315162
申请日：2005-12-23
申请人： Toshiyuki Yokosuka , Kinya Kobayashi , Kiyomi Yoshinari , Atsushi Otake , Atsumu Hirabayashi , Yasushi Terui
发明人： Toshiyuki Yokosuka , Kinya Kobayashi , Kiyomi Yoshinari , Atsushi Otake , Atsumu Hirabayashi , Yasushi Terui
IPC分类号： H01J49/00
CPC分类号： H01J49/004 , H01J49/0031
摘要： The present invention can provide a mass spectrometric system judging whether a measurement target is a substance required by an operator within an actual measurement time, when a substance (particularly such as protein or sugar chains) is analyzed. In the mass spectrometric system using a tandem mass spectrometer, a particular substance obtained by separating a sample is ionized, and mass analysis of the ionized substance is performed to obtain a spectrum. This spectrum is compared with a particular spectrum stored in advance, to thereby determine whether both the spectra match with each other. When a match is determined, a particular ion is further ionized within a particular time for detailed analysis. The invention also provides a mass spectrometric method, a diagnosis system and an inspection system each using the mass spectrometric system, and a program for operating a computer to control those systems with desired functions.
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公开(公告)号：US06957159B2
公开(公告)日：2005-10-18
申请号：US10643545
申请日：2003-08-19
申请人： Kinya Kobayashi , Kiyomi Yoshinari , Atsushi Otake
发明人： Kinya Kobayashi , Kiyomi Yoshinari , Atsushi Otake
IPC分类号： G01N27/62 , G01N33/483 , G01N33/68 , H01J49/04 , H01J49/40 , H01J49/42 , G06F19/00 , G01N31/00
CPC分类号： H01J49/0036
摘要： An apparatus for analyzing mass spectrometric data is described. The apparatus has a first input section for entering first data of an ion measured by mass spectrometry, a second input section for entering second data of a dissociated ion of the ion measured by mass spectrometry, a first data storing section for storing third data of mass spectrometry of a plurality of candidates for the structure of ion, a calculation section for producing fourth data of mass spectrometry of dissociated ions to be used in analyzing the plurality of candidates and an evaluation section for evaluating the plurality of candidates by making comparisons between the first and third data and between the second and fourth data, so that the structure of ion can be identified.
摘要翻译： 描述了用于分析质谱数据的装置。 该装置具有用于输入通过质谱法测定的离子的第一数据的第一输入部，用于输入通过质谱法测定的离子的离解离子的第二数据的第二输入部，存储质量的第三数据的第一数据存储部 用于离子结构的多个候选物的光谱测定，用于产生用于分析多个候选物的离解离子的质谱的第四数据的计算部分和用于通过比较第一 和第三数据以及第二和第四数据之间，从而可以识别离子的结构。
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公开(公告)号：US20050184232A1
公开(公告)日：2005-08-25
申请号：US11041864
申请日：2005-01-25
CPC分类号： H01J49/0036 , H01J49/004
摘要： According to the existing mass spectrometric system, whether or not the informations are sufficient for analyzing substances (particularly proteins, sugars, etc.) cannot be judged in the process of measurement. Further, it is difficult to find out isomers having just the same mass number or compounds very close in mass only from the MS data. According to this invention, whether or not the retention time in the LC (or GC) of peptide formed at the time of enzymatic decomposition of protein coincides with the predicted retention time assumed from the amino acid sequence predicted from MS2 mass spectrometry data is judged within the actual time period of measurement, and thereby the quality of MS2 mass spectrometry data (quantity of information) is judged.
摘要翻译： 根据现有的质谱系统，不能在测量过程中判断信息是否足以分析物质（特别是蛋白质，糖等）。 此外，难以从MS数据中发现质量数量相同或质量非常接近的异构体。 根据本发明，在蛋白质酶促分解时形成的肽的LC（或GC）中的保留时间是否与从MS2预测的氨基酸序列假定的预测保留时间一致， SUP>质谱数据在实际测量时间段内进行判断，从而判断MS质谱质谱数据（信息量）的质量。
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28.发明授权公开(公告)号：US06907352B2
公开(公告)日：2005-06-14
申请号：US10253481
申请日：2002-09-25
申请人： Kiyomi Yoshinari , Kinya Kobayashi , Lee Chahn
发明人： Kiyomi Yoshinari , Kinya Kobayashi , Lee Chahn
CPC分类号： H01J49/0036 , G06F19/16 , G06F19/703
摘要： A main object is to cope with an unknown structure substance thereby to identify the structure of a parent ion highly precisely and to derive a supposed structure. A method for analyzing mass spectrometric data is disclosed, which: acquires mass spectrometric data on an ionized sample and dissociated ions dissociated from the sample as a parent ion; derives dissociated ion candidates by analyzing the molecular orbits on the candidates of the structures of the parent ion; and displays the analytical results of the parent ion candidates and the dissociated ion candidates and compares the data of the dissociated ion candidates and the data of dissociated ions actually measured, to evaluate the structures of the parent ion candidates.
摘要翻译： 主要目的是应对未知的结构物质，从而高度精确地鉴定母体离子的结构，并得出假定的结构。 公开了一种用于分析质谱数据的方法，其获取离子化样品和离开样品离解的离子离子作为母离子的质谱数据; 通过分析母体离子结构的候选物上的分子轨道衍生离解离子候选物; 并显示母体离子候选物和解离离子候选物的分析结果，并比较实际测量的离解离子候选物和离解离子数据的数据，以评估母离子候选物的结构。
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公开(公告)号：US20050063864A1
公开(公告)日：2005-03-24
申请号：US10849517
申请日：2004-05-20
申请人： Akihiro Sano , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari , Kenko Uchida
发明人： Akihiro Sano , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari , Kenko Uchida
CPC分类号： G01N33/6851 , G01N33/6848 , H01J49/0031 , H01J49/0036 , H01J49/004
摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.
摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和来自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MS
光谱中的信息在涉及多级解离和质谱（MS n）的MS n的每个步骤中被有效地利用，使得用于确定下一个分析内容的流 并且可以在测量的实时和高效率和精确度下优化用于MS 分析的母体离子的选择。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。 -
公开(公告)号：US09143025B2
公开(公告)日：2015-09-22
申请号：US13538490
申请日：2012-06-29
申请人： Norihisa Iwasaki , Yuji Enomoto , Junnosuke Nakatsugawa , Masashi Kitamura , Kinya Kobayashi , Hironori Matsumoto
发明人： Norihisa Iwasaki , Yuji Enomoto , Junnosuke Nakatsugawa , Masashi Kitamura , Kinya Kobayashi , Hironori Matsumoto
IPC分类号： H02K49/10
CPC分类号： H02K49/102
摘要： A magnetic gear mechanism including a simplified assembly of a magnetic flux modulating section in the magnetic gear mechanism which improves the strength thereof. In the magnetic flux modulating section of the magnetic gear mechanism, the magnetic flux modulating section being formed of a magnetic member and a non-magnetic member, a piece of the magnetic member and a piece of the non-magnetic member are separately produced. The piece of the magnetic member is sandwiched between circumferential projections provided in pieces of the non-magnetic member, and the magnetic member and the non-magnetic member and bearing holding sections form a structure in which the magnetic member and the non-magnetic member are fitted into the bearing holding sections by using recessed portions provided in the bearing holding sections and axial projections provided in the pieces of the non-magnetic member. This structure simplifies production and improves strength.
摘要翻译： 一种磁齿轮机构，其包括在磁齿轮机构中的磁通量调节部的简化组合，其提高其强度。 在磁齿轮机构的磁通量调制部中，由磁性部件和非磁性部件构成的磁通量调制部分分别制造磁性部件和非磁性部件。 磁性部件被夹在设置在非磁性部件之间的周向凸起之间，磁性部件和非磁性部件以及轴承保持部分形成磁性部件和非磁性部件的结构 通过设置在轴承保持部中的凹部和设置在非磁性构件的片中的轴向突起而装配到轴承保持部中。 该结构简化了生产并提高了强度。
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