公开(公告)号：US20110284733A1

公开(公告)日：2011-11-24

申请号：US12785945

申请日：2010-05-24

Applicant: Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming Lee Chu

Inventor： Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming Lee Chu

IPC: H01J49/26 ,  B01D59/44

CPC classification number: H01J49/025 ,  H01J49/0027

Abstract: A mass spectrometer and methods for obtaining the mass spectrum of a single macromolecular or biomolecular ion in a mass spectrometer. The methods include creating single macromolecular or biomolecular primary ions in an ion trap by ionization of a macromolecule or biomolecule; ejecting half of the primary ions for detection with a first charge detector; ejecting half of the primary ions to impact upon a conversion dynode, thereby creating secondary ions for detection with charge amplification detector such as a channeltron or an electromultiplier or an MCP.

Abstract translation: 质谱仪和在质谱仪中获得单个大分子或生物分子离子的质谱的方法。 该方法包括通过大分子或生物分子的离子化在离子阱中产生单个大分子或生物分子一级离子; 用第一电荷检测器喷射一半的初级离子用于检测; 喷射一半的初级离子以冲击转换倍增极，从而产生二次离子，以便用电荷放大检测器（例如通道电极或电倍增管或MCP）进行检测。

公开(公告)号：US20110147581A1

公开(公告)日：2011-06-23

申请号：US12976543

申请日：2010-12-22

Applicant: Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

Inventor： Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

IPC: H01J49/26

CPC classification number: H01J49/0022 ,  H01J49/424 ,  H01J49/429

Abstract: Methods and apparatuses for portable mass spectrometry are disclosed. The apparatuses comprise at least one source of ionized analyte, at least one frequency scanning subsystem, at least one detector, and optionally at least one vacuum pump, and are portable. In some embodiments, the apparatuses comprise multiple sources of ionized analyte and/or are configured to obtain mass spectra of a large analyte, such as analyte with an m/z ratio of at least 105, or analyte with a molecular weight of at least 105 Da, as well as mass spectra of small molecule analyte. In some embodiments, the methods comprise obtaining mass spectra with a portable apparatus described above.

Abstract translation: 公开了便携式质谱法的方法和装置。 该装置包括至少一个离子化分析物源，至少一个频率扫描子系统，至少一个检测器和可选的至少一个真空泵，并且是便携式的。 在一些实施方案中，装置包括多个电离分析物源和/或配置成获得大分析物的质谱，例如m / z比至少为105的分析物，或分子量至少为105的分析物 Da，以及小分子分析物的质谱。 在一些实施例中，所述方法包括使用上述便携式装置获得质谱。

公开(公告)号：US20100301199A1

公开(公告)日：2010-12-02

申请号：US12457064

申请日：2009-05-29

Applicant: Chung-Hsuan Chen ,  Chen-I Wu

Inventor： Chung-Hsuan Chen ,  Chen-I Wu

IPC: B01D59/44 ,  H01J49/00

CPC classification number: H01J49/10 ,  H01J49/0454

Abstract: Methods and systems for ultrasound ionization mass spectrometry are provided. Analytes in a sample are ionized by subjecting them to ultrasound, facilitating their analysis by mass spectrometry. With these methods and systems, soft ionization of large analytes, including biological macromolecules and nanoparticles, can be achieved. Ionization efficiency can be improved by addition of chemicals such as, for example, organic solvents or acids to the sample.

Abstract translation: 提供了超声电离质谱法的方法和系统。 样品中的分析物通过对其进行超声波离子化，便于通过质谱法进行分析。 通过这些方法和系统，可以实现大分子的软电离，包括生物大分子和纳米颗粒。 通过向样品中加入化学物质，例如有机溶剂或酸，可以提高电离效率。

公开(公告)号：US20080188374A1

公开(公告)日：2008-08-07

申请号：US12022553

申请日：2008-01-30

Applicant: Chung-Hsuan Chen ,  Yu-Chuan Chien

Inventor： Chung-Hsuan Chen ,  Yu-Chuan Chien

IPC: C40B20/00 ,  C40B60/10

CPC classification number: C40B40/06 ,  C40B20/04 ,  C40B60/10

Abstract: This invention relates to a kit and a system for performing an in-solution microarray assay, and a method of carrying out this assay. The kit includes a plate having a number of wells in which a plurality of magnetic beads are placed. These magnetic beads are coated with different probes, wherein beads placed in each individual well are coated with identical probes.

Abstract translation: 本发明涉及用于进行溶液中微阵列测定的试剂盒和系统，以及进行该测定的方法。 该套件包括具有许多孔的板，其中放置有多个磁珠。 这些磁珠用不同的探针涂覆，其中放置在每个单独的孔中的珠子用相同的探针包被。

公开(公告)号：US20080078928A1

公开(公告)日：2008-04-03

申请号：US11542568

申请日：2006-10-03

Applicant: Yi-Sheng Wang ,  Chung-Hsuan Chen ,  Shang-Ting Tsai ,  Chiu Wen Chen

Inventor： Yi-Sheng Wang ,  Chung-Hsuan Chen ,  Shang-Ting Tsai ,  Chiu Wen Chen

IPC: H01J49/40

CPC classification number: H01J49/40 ,  H01J49/0095

Abstract: A dual-polarity mass spectrometer includes an ion source, a negative ion mass analyzer, and a positive ion mass analyzer to measure both the negative and positive ion spectra of a sample material simultaneously. The ion source includes a sample surface on which the sample material is positioned, the sample material providing positive ions and negative ions when excited by a laser beam or an energetic particle stream. A first extraction electrode is connected to a voltage higher than the sample surface to attract the negative ions from the sample electrode. A second extraction electrode is connected to a voltage lower than the sample surface to attract the positive ions from the sample electrode. The negative and positive ions are analyzed simultaneously by the negative ion mass analyzer and the positive ion mass analyzer, respectively.

Abstract translation: 双极性质谱仪包括离子源，负离子质量分析仪和正离子质量分析仪，以同时测量样品材料的负离子和正离子光谱。 离子源包括其上放置样品材料的样品表面，当由激光束或能量粒子流激发时，样品材料提供正离子和负离子。 第一引出电极连接到高于样品表面的电压以吸引来自样品电极的负离子。 第二提取电极连接到低于样品表面的电压，以吸引来自样品电极的正离子。 负离子和正离子分别通过负离子质量分析仪和正离子质量分析仪同时分析。