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公开(公告)号：US06908763B1
公开(公告)日：2005-06-21
申请号：US08918537
申请日：1997-08-22
IPC分类号： A61K38/00 , C12N5/0781 , C12N5/0789 , C12N5/02 , C12N5/06 , C12N5/08 , C12N15/87
CPC分类号： C12N5/0635 , A61K38/00 , C12N5/0647 , C12N2501/125 , C12N2501/23 , C12N2501/26 , C12N2510/00
摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for lymphoid lineages, but lacking the potential to differentiate into myeloid and erythroid lineages. Methods are provided for the isolation and culture of this common lymphoid progenitor cell (CLP). The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor α); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. The murine cells are also characterized as expressing low levels of sca-1 (Ly-6E and Ly-6A). The CLPs are predominantly cycling, blast cells. These cells give rise to B cells, T cells and natural killer cells, as evidenced by their growth and differentiation in vitro and in vivo.
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22.发明授权
公开(公告)号：US5087570A
公开(公告)日：1992-02-11
申请号：US192378
申请日：1988-05-10
CPC分类号： A61K35/28 , C07K14/52 , G01N33/5005 , Y10S436/808
摘要： Highly concentrated hematopoietic stem cell compositions are provided which are substantially free of differentiated or dedicated hematopoietic cells. The cells are obtained by subtraction of cells having particular markers and selection of cells having particular markers. The resulting composition may be used to provide for individual or groups of hematopoietic lineages, to reconstitute stem cells of the host, and to identify an assay for a wide variety of hematopoietic growth factors.
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23.发明授权
公开(公告)号：US4786590A
公开(公告)日：1988-11-22
申请号：US691997
申请日：1985-01-15
IPC分类号： A61K47/48 , C07K16/28 , G01N33/50 , G01N33/569 , A61K39/00 , G01N33/574 , G01N33/577
CPC分类号： G01N33/5091 , A61K47/48561 , C07K16/28 , C07K2317/73 , Y10S436/813
摘要： Specific binding members are provided for binding to lymphocytic cell surface receptors, which receptors are involved in the activation of a cell from the G.sub.0 state into the cell division cycle to proliferate. The cell surface receptors are further characterized by binding to envelope proteins of neoplasia-causing retroviruses, and/or to intact retroviral particles.
摘要翻译： 提供特异性结合成员用于结合淋巴细胞表面受体，该受体参与将细胞从G0状态激活至细胞分裂周期以增殖。 细胞表面受体的进一步特征在于结合产生引起肿瘤的逆转录病毒的包膜蛋白和/或完整的逆转录病毒颗粒。
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公开(公告)号：US09175079B2
公开(公告)日：2015-11-03
申请号：US13575511
申请日：2011-01-28
申请人： Chad Tang , Irving L. Weissman , Micha Drukker
发明人： Chad Tang , Irving L. Weissman , Micha Drukker
CPC分类号： C07K16/28 , C12N5/0081
摘要： Compositions and methods are provided for depletion of pluripotent cells. In one embodiment of the invention, methods are provided for depletion of pluripotent cells from a mixed population of differentiated cells and stem cells, to provide a population of cells substantially free of pluripotent stem cells. Monoclonal antibodies useful in depletion and in identification of pluripotent stem cells are also provided.
摘要翻译： 提供了多能细胞耗竭的组合物和方法。 在本发明的一个实施方案中，提供了从分化细胞和干细胞的混合群体中消耗多能细胞的方法，以提供基本上不含多能干细胞的细胞群。 还提供了可用于多能干细胞的消耗和鉴定中的单克隆抗体。
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公开(公告)号：US20130028909A1
公开(公告)日：2013-01-31
申请号：US13575511
申请日：2011-01-28
申请人： Chad Tang , Irving L. Weissman , Micha Drukker
发明人： Chad Tang , Irving L. Weissman , Micha Drukker
IPC分类号： A61K39/395 , A61P35/00 , C07K16/28 , C12N5/071
CPC分类号： C07K16/28 , C12N5/0081
摘要： Compositions and methods are provided for depletion of pluripotent cells. In one embodiment of the invention, methods are provided for depletion of pluripotent cells from a mixed population of differentiated cells and stem cells, to provide a population of cells substantially free of pluripotent stem cells. Monoclonal antibodies useful in depletion and in identification of pluripotent stem cells are also provided.
摘要翻译： 提供了多能细胞耗竭的组合物和方法。 在本发明的一个实施方案中，提供了从分化细胞和干细胞的混合群体中消耗多能细胞的方法，以提供基本上不含多能干细胞的细胞群。 还提供了可用于多能干细胞的消耗和鉴定中的单克隆抗体。
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26.发明申请Cell Surface Marker Expression in Hematopoietic Stem Cells and Progenitors for the Diagnosis, Prognosis, and Treatment of Myelodysplastic Syndromes 审中-公开造血干细胞和祖细胞中的细胞表面标记表达用于骨髓增生异常综合征的诊断，预后和治疗公开(公告)号：US20120283124A1
公开(公告)日：2012-11-08
申请号：US13508319
申请日：2010-11-09
IPC分类号： G01N33/574 , C40B30/04 , C12Q1/68 , C12N5/095
CPC分类号： G01N33/57426 , G01N33/56966
摘要： Myelodysplastic syndromes are staged by analysis of the presence of hematopoietic stem and/or progenitor cells, particularly progenitor cells dedicated to the myeloid lineage and hematopoietic stem cells.
摘要翻译： 骨髓增生异常综合征是通过分析造血干细胞和/或祖细胞，特别是专用于髓系谱系和造血干细胞的祖细胞的分析进行的。
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公开(公告)号：US20110076683A1
公开(公告)日：2011-03-31
申请号：US12875025
申请日：2010-09-02
IPC分类号： C12Q1/68 , C12N5/095 , C12Q1/04 , G01N33/574 , C12Q1/02
CPC分类号： G01N33/57426 , C12N5/0695 , G01N33/5011 , G01N2333/70589 , G01N2333/70596 , G01N2333/715
摘要： Isolated populations of leukemic stem cells are provided. The cells are useful for experimental evaluation, and as a source of lineage and cell specific products, and as targets for the discovery of factors or molecules that can affect them. Detection of leukemic stem cells is useful in predicting disease progression, relapse, and development of drug resistance. Proliferation of LSC may be inhibited through interfering with activation of the β-catenin pathway. Methods are provided for the clinical staging of pre-leukemia and leukemias by differential analysis of hematologic samples for the distribution of one or more hematopoietic stem or progenitor cell subsets.
摘要翻译： 提供了白血病干细胞的分离群体。 这些细胞可用于实验评价，也可用作谱系和细胞特异性产物的来源，也可用于发现影响它们的因子或分子。 白血病干细胞的检测可用于预测疾病进展，复发和耐药发展。 LSC的增殖可能通过干扰β-联蛋白途径的活化来抑制。 通过差异分析血液样品以分配一个或多个造血干细胞或祖细胞亚群，为白血病和白血病的临床分期提供方法。
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公开(公告)号：US20110015090A1
公开(公告)日：2011-01-20
申请号：US12836152
申请日：2010-07-14
CPC分类号： C07K16/2803 , A61K2039/505 , A61K2039/507 , C07K16/28 , C07K16/2896 , C07K16/30 , C07K2317/24 , C07K2317/73 , C07K2317/75 , C07K2317/76 , C12N5/0093 , C12N5/0694 , C12Q1/6886 , C12Q2600/112 , C12Q2600/136 , G01N33/5011 , G01N33/57426 , G01N2500/04
摘要： Markers of acute myeloid leukemia stem cells (AMLSC) are identified. The markers are differentially expressed in comparison with normal counterpart cells, and are useful as diagnostic and therapeutic targets.
摘要翻译： 确定急性骨髓性白血病干细胞（AMLSC）的标志物。 标记与正常对照细胞相比差异表达，可用作诊断和治疗靶点。
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公开(公告)号：US20100267079A1
公开(公告)日：2010-10-21
申请号：US12802968
申请日：2010-06-16
申请人： Irving L. Weissman , Keith S. Chan
发明人： Irving L. Weissman , Keith S. Chan
CPC分类号： G01N33/57426 , C12N5/0695 , G01N2333/70585
摘要： Transitional cell carcinoma stem cells (TCCSC) are identified. The cells can be prospectively isolated or identified from primary tumor samples, and are shown to possess the unique properties of cancer stem cells in functional assays for cancer stem cell self-renewal and differentiation, and in cancer diagnosis.
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公开(公告)号：US07641897B2
公开(公告)日：2010-01-05
申请号：US11286088
申请日：2005-11-23
CPC分类号： C12N5/0606 , C12N2500/90 , C12N2501/235 , C12N2501/48 , C12N2510/00
摘要： Undifferentiated primordial stem cells are manipulated to permit their long term growth in defined media lacking serum and feeder layer cells by shifting the apoptotic balance of the cells, through increasing the activity of Bcl-2 family anti-apoptotic proteins or decreasing the activity of Bcl-2 family pro-apoptotic proteins. In some embodiments of the invention, the Bcl family protein is Bcl-2. The ES cells sustain the characteristics of undifferentiated, pluripotent stem cells during long-term serum- and feeder layer cell-free conditions, including the ability to be expanded in vitro, but maintain their potential to differentiate into mature cell types.
摘要翻译： 通过改变细胞的凋亡平衡，通过增加Bcl-2家族抗凋亡蛋白的活性或降低Bcl-2家族的抗凋亡蛋白的活性，操作未分化的原始干细胞以允许其在缺乏血清和饲养层细胞的限定培养基中长期生长， 2家庭促细胞凋亡蛋白。 在本发明的一些实施方案中，Bcl家族蛋白是Bcl-2。 ES细胞在长期血清和饲养层无细胞条件下维持未分化，多能干细胞的特征，包括体外扩增能力，但保持其分化成成熟细胞类型的潜力。
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