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21.发明授权公开(公告)号：US06486314B1
公开(公告)日：2002-11-26
申请号：US09604957
申请日：2000-06-28
IPC分类号： C07H1100
CPC分类号： C12P19/18 , A23L29/269 , A23Y2220/71 , C08B37/0009 , C12N9/1051 , C12N9/107
摘要： The present invention describes a protein having glucosyltransferase activity. This protein is derived from lactobacilli, which are food-grade microorganisms with the Generally Recognized As Safe (GRAS) status. The protein produces a glucan with a unique structure having 4-linked, 6-lined and 4,6-linked anhydroglucose units or in the presence of suitable acceptors, oligosaccharides. According to the invention lactobacilli capable of producing this glucan using the novel glucosyltransferase can be used as a probiotic or symbiotic.
摘要翻译： 本发明描述了具有葡糖基转移酶活性的蛋白质。 该蛋白质来自乳酸杆菌，其是具有通用认可安全（GRAS）状态的食品级微生物。 该蛋白质产生具有独特结构的葡聚糖，其具有4-连接，6-内衬和4,6-连接的脱水葡萄糖单元，或在合适的受体寡糖的存在下。 根据本发明，使用新型葡糖基转移酶能够产生该葡聚糖的乳杆菌可用作益生菌或共生菌。
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22.发明授权Identification of 3-ketosteroid 9-alfa-hydroxylase genes and microorganisms blocked in 3-ketosteroid 9-alfa-hydroxylase activity 失效鉴定3-酮类固醇9-alfa-羟化酶基因和微生物在3-酮类固醇9-α-羟化酶活性中被阻断公开(公告)号：US07514236B2
公开(公告)日：2009-04-07
申请号：US11654748
申请日：2007-01-18
CPC分类号： C12N9/0071 , C12P33/00
摘要： The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence encoding the amino acid sequence of KshA protein or of KshB protein, encoded by nucleotides 499-1695 of SEQ ID NO:1 or by nucleotides 387-1427 of SEQ ID NO:2, respectively, and functional homologues thereof.The polynucleotides of the invention can be used to construct genetically modified microorganisms blocked in 3-ketosteroid 9α-hydroxylase activity, which are useful in the microbial degradation of steroids to accumulate certain steroid products.
摘要翻译： 本发明涉及分离的多核苷酸序列，其包含编码由SEQ ID NO：1的核苷酸499-1695或SEQ ID NO：2的核苷酸387-1427编码的KshA蛋白或KshB蛋白的氨基酸序列的核酸序列 ，及其功能同系物。 本发明的多核苷酸可用于构建在3-酮类固醇9α-羟化酶活性中封闭的遗传修饰微生物，其可用于类固醇的微生物降解以积聚某些类固醇产物。
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23.发明申请Identification of 3-Ketosteroid 9-Alfa-hydroxylase genes and microorganisms blocked in 3-Ketosteroid 9-Alfa-hydroxylase activity 失效鉴定3-酮类固醇9-Alfa-羟化酶基因和微生物在3-酮类固醇9-Alfa-羟化酶活性中被阻断公开(公告)号：US20060040343A1
公开(公告)日：2006-02-23
申请号：US10504034
申请日：2003-02-19
CPC分类号： C12N9/0071 , C12P33/00
摘要： The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence encoding the amino acid sequence of KshA protein or of KshB protein, encoded by nucleotides 499-1695 of SEQ ID NO:1 or by nucleotides 387-1427 of SEQ ID NO:2, respectively, and functional homologues thereof. The polynucleotides of the invention can be used to construct genetically modified microorganisms blocked in 3-ketosteroid 9α-hydroxylase activity, which are useful in the microbial degradation of steroids to accumulate certain steroid products.
摘要翻译： 本发明涉及分离的多核苷酸序列，其包含编码由SEQ ID NO：1的核苷酸499-1695或SEQ ID NO：2的核苷酸387-1427编码的KshA蛋白或KshB蛋白的氨基酸序列的核酸序列 ，及其功能同系物。 本发明的多核苷酸可用于构建在3-酮类固醇9α-羟化酶活性中封闭的遗传修饰微生物，其可用于类固醇的微生物降解以积聚某些类固醇产物。
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公开(公告)号：US6004790A
公开(公告)日：1999-12-21
申请号：US947965
申请日：1997-10-09
CPC分类号： C12N9/1074 , C12P19/18 , C12Y204/01019
摘要： The present invention relates to variants of cyclomaltodextrin glucanotransferase. More specifically the invention relates to a method of modifying the substrate binding and/or product selectivity of a precursor CGTase enzyme, and CGTase variants derived from a precursor CGTase enzyme by substitution, insertion and/or deletion of one or more amino acid residue(s), which amino acid residue(s) holds a position close to the substrate. Moreover, the invention relates to DNA constructs encoding the CGTase variants, expression vectors, host cells and methods of producing the CGTase variants of the invention.
摘要翻译： 本发明涉及环糊精葡聚糖转移酶的变体。 更具体地，本发明涉及一种通过取代，插入和/或缺失一个或多个氨基酸残基来修饰前体CGT酶的底物结合和/或产物选择性的方法和衍生自前体CGT酶的CGTase变体（s ），其中氨基酸残基保持靠近底物的位置。 此外，本发明涉及编码CGTase变体，表达载体，宿主细胞的DNA构建体和产生本发明的CGTase变体的方法。
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