公开(公告)号：US08012729B2

公开(公告)日：2011-09-06

申请号：US12861734

申请日：2010-08-23

申请人： Hideaki Yamaoka

发明人： Hideaki Yamaoka

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/26 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12Q1/32

摘要： Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.

摘要翻译： 通过用另一个氨基酸残基取代位置472和/或475处的氨基酸残基来提高具有SEQ ID NO：13氨基酸序列的葡萄糖脱氢酶对葡萄糖的葡萄糖的底物特异性。

公开(公告)号：US07824881B2

公开(公告)日：2010-11-02

申请号：US12008358

申请日：2008-01-10

申请人： Hideaki Yamaoka ,  Mitsuhiro Hoshijima ,  Tomomichi Tsujimoto

发明人： Hideaki Yamaoka ,  Mitsuhiro Hoshijima ,  Tomomichi Tsujimoto

IPC分类号： C12Q1/32

CPC分类号： G01N27/3272 ,  C12Q1/004 ,  C12Q1/006 ,  C12Q1/32

摘要： The present invention relates to a technique for measuring a glucose level by utilizing a reaction system containing an enzyme and an electron carrier. In accordance with the glucose level measuring method of the present invention, glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier. The present invention further provides a glucose sensor in which glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier.

摘要翻译： 本发明涉及利用含有酶和电子载体的反应体系来测量葡萄糖水平的技术。 根据本发明的葡萄糖水平测定方法，使用附着有细胞色素C的葡萄糖脱氢酶或来自属于伯克霍尔德氏菌属的微生物的葡萄糖脱氢酶作为酶，使用Ru化合物作为电子载体。 本发明还提供一种葡萄糖传感器，其中使用连接有细胞色素C的葡萄糖脱氢酶或衍生自属于伯克霍尔德氏菌属的微生物的葡萄糖脱氢酶作为酶，并且使用Ru化合物作为电子载体。

公开(公告)号：US07713718B1

公开(公告)日：2010-05-11

申请号：US10526049

申请日：2003-08-20

申请人： Koji Sode ,  Hideaki Yamaoka ,  Mitsuhiro Hoshijima ,  Keisuke Kurosaka ,  Shido Kawase

发明人： Koji Sode ,  Hideaki Yamaoka ,  Mitsuhiro Hoshijima ,  Keisuke Kurosaka ,  Shido Kawase

IPC分类号： C12N1/21 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12N9/0004

摘要： A process for producing glucose dehydrogenases. This process comprises transferring a DNA containing a sequence represented by SEQ ID NO:1 which encodes an α subunit having a glucose dehydrogenase activity and a β subunit being an electron transfer protein into a microorganism belonging to the genus Pseudomonas to thereby construct a transformant, and culturing this transformant so as to allow the production of a first glucose dehydrogenase containing the above-described β subunit and a second glucose dehydrogenase free from the β subunit. The α subunit as described above has a molecular weight of about 60 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions, while the β subunit as described above has a molecular weight of about 43 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions.

摘要翻译： 葡萄糖脱氢酶的生产方法。 该方法包括转移含有编码具有葡萄糖脱氢酶活性的α亚单位的SEQ ID NO：1表示的序列的DNA， 亚基是向属于假单胞菌属的微生物的电子转移蛋白质，从而构建转化体，并培养该转化体，以便制备含有上述＆lt; B＆gt;的第一葡萄糖脱氢酶。 亚基和不含“bgr”的第二葡萄糖脱氢酶 亚基。 如上所述的α亚基通过例如在还原条件下的SDS-聚丙烯酰胺凝胶电泳测量的分子量为约60kDa，而＆bgr; 亚单位通过例如在还原条件下的SDS-聚丙烯酰胺凝胶电泳测量的分子量为约43kDa。

公开(公告)号：US07604969B2

公开(公告)日：2009-10-20

申请号：US11665296

申请日：2006-06-12

申请人： Hideaki Yamaoka ,  Masashi Tsukada

发明人： Hideaki Yamaoka ,  Masashi Tsukada

IPC分类号： C12N9/04 ,  C12Q1/32 ,  C12Q1/00 ,  C12P21/04 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/32 ,  C12N9/0006 ,  C12Q1/54 ,  C12Y101/01049

摘要： A mutant glucose dehydrogenase having an improved substrate specificity to glucose, which is a protein comprising the amino acid sequence depicted in SEQ ID NO:3 or an amino acid sequence having the substitution, deletion, insertion or addition of one or more amino acid residues excluding amino acid 365 in the amino acid sequence depicted in SEQ ID NO:3 and having a glucose dehydrogenase activity, the amino acid residue corresponding to amino acid 365 being substituted by other amino acid residue.

摘要翻译： 具有改善的对葡萄糖的底物特异性的突变体葡萄糖脱氢酶，其是包含SEQ ID NO：3所示的氨基酸序列的蛋白质或具有取代，缺失，插入或添加一个或多个氨基酸残基的氨基酸序列， SEQ ID NO：3所示的氨基酸序列中的氨基酸365，具有葡萄糖脱氢酶活性，对应于氨基酸365的氨基酸残基被其它氨基酸残基取代。

公开(公告)号：US20080206833A1

公开(公告)日：2008-08-28

申请号：US11665296

申请日：2006-06-12

申请人： Hideaki Yamaoka ,  Masashi Tsukada

发明人： Hideaki Yamaoka ,  Masashi Tsukada

IPC分类号： C12N9/04 ,  C07H21/04 ,  C12N1/00

CPC分类号： C12Q1/32 ,  C12N9/0006 ,  C12Q1/54 ,  C12Y101/01049

摘要： A mutant glucose dehydrogenase having an improved substrate specificity to glucose, which is a protein comprising the amino acid sequence depicted in SEQ ID NO:3 or an amino acid sequence having the substitution, deletion, insertion or addition of one or more amino acid residues excluding amino acid 365 in the amino acid sequence depicted in SEQ ID NO:3 and having a glucose dehydrogenase activity, the amino acid residue corresponding to amino acid 365 being substituted by other amino acid residue.

摘要翻译： 具有改善的对葡萄糖的底物特异性的突变体葡萄糖脱氢酶，其是包含SEQ ID NO：3所示的氨基酸序列的蛋白质或具有取代，缺失，插入或添加一个或多个氨基酸残基的氨基酸序列， SEQ ID NO：3所示的氨基酸序列中的氨基酸365，具有葡萄糖脱氢酶活性，对应于氨基酸365的氨基酸残基被其它氨基酸残基取代。

公开(公告)号：US20060191813A1

公开(公告)日：2006-08-31

申请号：US10550287

申请日：2004-03-25

申请人： Hideaki Yamaoka

发明人： Hideaki Yamaoka

IPC分类号： B65D85/00

CPC分类号： G01N33/48778 ,  C12Q1/004

摘要： A sensor storage container is provided that allows sensors to be checked visually from the outside. A container 1 is used for storing sensors 14 including an oxidation-reduction enzyme that oxidizes or reduces an object to be analyzed, a mediator that mediates the transfer of electrons caused by oxidation or reduction, and a detection means that detects the oxidation or reduction reaction. The container 1 is transparent or semi-transparent in whole or in part and thereby the sensors can be checked visually from the outside of the container. This container 1 may be composed of a container body 11 and a lid 12. The container body 11 may be provided with a scale 13. Preferably, the sensors are those produced using a ruthenium metal complex as the mediator.

摘要翻译： 提供了一种传感器存储容器，其允许从外部视觉检查传感器。 容器1用于存储包含氧化或还原待分析对象的氧化还原酶的传感器14，介导由氧化还原引起的电子传递的介体和检测氧化还原反应的检测装置 。 容器1全部或部分透明或半透明，从而可以从容器的外部视觉检查传感器。 该容器1可以由容器主体11和盖12构成。 容器主体11可以设置有刻度13。 优选地，传感器是使用钌金属络合物作为介体产生的传感器。

公开(公告)号：US07063775B2

公开(公告)日：2006-06-20

申请号：US10276473

申请日：2001-05-15

申请人： Hideaki Yamaoka

发明人： Hideaki Yamaoka

IPC分类号： G01N27/327

CPC分类号： G01N27/3272 ,  C12Q1/002 ,  G01N2333/904 ,  Y10T29/49002

摘要： A biosensor (I) which comprises a capillary (3) having a passage (30) fined with an inlet (30a) for taking a sample solution therein, a membrane (8) for accelerating the transference of a sample solution in the passage (30), and a reaction portion (7) containing a reagent which reacts with a component to be detected in a sample solution.

摘要翻译： 一种生物传感器（I），其包括具有通道（30）的毛细管（3），所述通道（30）具有用于在其中取样品溶液的入口（30a），用于加速所述通道中的样品溶液的转移的膜（8） 30）和含有与样品溶液中待检测组分反应的试剂的反应部分（7）。