公开(公告)号：US20170073661A1

公开(公告)日：2017-03-16

申请号：US15363038

申请日：2016-11-29

申请人： Roche Diabetes Care, Inc.

发明人： Wolfgang Roedel ,  Carina Horn ,  Nelli Steinke ,  Nadine Bucci ,  Thomas Meier ,  Rainer Schmuck ,  Rolf Nagel ,  Dieter Heindl

IPC分类号： C12N9/96 ,  C12Q1/26 ,  C12N9/02 ,  C12Q1/32 ,  C12N9/04

CPC分类号： C12N9/96 ,  C12N9/0006 ,  C12N9/0036 ,  C12Q1/26 ,  C12Q1/32 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01047 ,  C12Y101/01049 ,  C12Y106/05002 ,  C12Y106/99

摘要： Methods are provided for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme. In addition, enzymes are provided that are stabilized with a stabilized coenzyme, as well as the use thereof in test elements for detecting analytes. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.

摘要翻译： 提供了通过在稳定的辅酶的存在下储存酶来稳定酶的方法。 此外，提供了用稳定化的辅酶稳定的酶以及其用于检测分析物的测试元件中的用途。 其他方面包括独特的组合物，方法，技术，涉及酶稳定化的系统和装置。

公开(公告)号：US20150089683A1

公开(公告)日：2015-03-26

申请号：US14395636

申请日：2013-03-06

申请人： Monsanto Technology LLC

发明人： Mark Scott Abad ,  Pranesh Badami ,  Amarjit Basra ,  Erin Bell ,  Christopher P. Bonin ,  Paolo Castiglioni ,  Eric R. Cerny ,  Jaishree M. Chittoor-Vijayanath ,  Paul Chomet ,  Todd Dezwaan ,  Stephen Duff ,  Michael D. Edgerton ,  Barry S. Goldman ,  Balasulojini Karunanandaa ,  Kempagangaiah Krishnamurthy ,  Ping Li ,  Adrian A. Lund ,  Llinda Lutfiyya ,  Thomas M. Malvar ,  Donald E. Nelson ,  Monnanda S. Rajani ,  Dhanalakshmi Ramachandra ,  Thomas G. Ruff ,  Sara Salvador ,  S. Sangeetha ,  Char Shobha ,  Jeffrey M. Staub ,  Virginia Ursin ,  Tyamagondlu V. Venkatesh ,  Kammaradi R. Vidya ,  Xiaoyun Wu ,  Sean Yang ,  Jianmin Zhao

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H1/02

CPC分类号： C12N15/8261 ,  A01H1/02 ,  C07K14/415 ,  C07K14/81 ,  C12N5/04 ,  C12N9/0006 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/825 ,  C12N15/8271 ,  C12N15/8273 ,  C12Y101/01049 ,  Y02A40/146

摘要： This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

摘要翻译： 本公开提供具有增强性状的转基因植物，例如增加的产量，增加的氮利用效率和增强的耐旱性; 这些转基因植物的繁殖体，后代和田间作物; 以及制备和使用这些转基因植物的方法。 本公开还提供了从这种转基因植物产生杂种种子的方法，种植这种种子并选择具有增强特征的后代植物。 还公开了具有改变的表型的转基因植物，其可用于筛选和选择所需增强性状的转基因事件。

公开(公告)号：US20100120105A1

公开(公告)日：2010-05-13

申请号：US12606410

申请日：2009-10-27

申请人： Larry Cameron Anthony ,  Michael Dauner ,  Gail K. Donaldson ,  Brian James Paul

发明人： Larry Cameron Anthony ,  Michael Dauner ,  Gail K. Donaldson ,  Brian James Paul

IPC分类号： C12P7/04 ,  C12N1/00 ,  C12N1/21 ,  C12N1/19

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/18 ,  C12N9/88 ,  C12N9/92 ,  C12N15/52 ,  C12Y101/01044 ,  C12Y101/01049 ,  C12Y207/02011 ,  C12Y401/02014 ,  C12Y402/02012 ,  Y02E50/10

摘要： A microbial host cell is provided for the production of isobutanol. Carbon flux in the cell is optimized through the Entner-Doudoroff pathway.

摘要翻译： 提供用于生产异丁醇的微生物宿主细胞。 通过Entner-Doudoroff途径优化细胞中的碳通量。

公开(公告)号：US07078204B2

公开(公告)日：2006-07-18

申请号：US10312007

申请日：2001-06-15

申请人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

发明人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12P21/04 ,  C12P13/04 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/08 ,  C12Y101/01049

摘要： The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant.According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

摘要翻译： 本发明涉及一种衍生自属于棒状杆菌属的细菌的新型葡萄糖-6-磷酸脱氢酶（以下称为“G6PD”），编码该酶的DNA，包含该DNA的重组DNA，包含重组体 DNA，包含其染色体上的DNA的转化体，以及生产L-氨基酸或G6PD的方法，其包括培养转化体。 根据本发明，获得了修饰的G6PD和编码G6PD的D​​NA，通过使用改良的G6PD可以提高微生物的L-氨基酸的生产率。

公开(公告)号：US20060014259A9

公开(公告)日：2006-01-19

申请号：US10091342

申请日：2002-03-06

申请人： Kevin Burke ,  Hermann Sahm ,  Lothar Eggeling ,  Bernd Moritz ,  L. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Bettina Mockel ,  Georg Thierbach ,  Rita Dunican

发明人： Kevin Burke ,  Hermann Sahm ,  Lothar Eggeling ,  Bernd Moritz ,  L. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Bettina Mockel ,  Georg Thierbach ,  Rita Dunican

IPC分类号： C12P13/08 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12N15/77 ,  C12P13/08 ,  C12Y101/01049

摘要： The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.

公开(公告)号：US20190055538A1

公开(公告)日：2019-02-21

申请号：US16179311

申请日：2018-11-02

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Yi Feng Zheng ,  Yali Yang ,  Linda Hudson ,  Nihmat Morjana

IPC分类号： C12N9/96 ,  G01N33/58 ,  C12N9/04 ,  G01N33/535

CPC分类号： C12N9/96 ,  C07K2319/00 ,  C12N9/0006 ,  C12Y101/01049 ,  G01N33/535 ,  G01N33/581

摘要： The present invention relates to multi-hapten mutant G6PDH conjugates, methods of their preparation and their use for detection of multiple analytes. The compositions of the invention comprise different types of haptens (molecules) require to be immobilized into the G6PDH to make one multi-hapten-G6PDH conjugate. Both thiol and amine functional groups on G6PDH are utilized for immobilization of different haptens.

公开(公告)号：US20180292421A1

公开(公告)日：2018-10-11

申请号：US15936158

申请日：2018-03-26

申请人： ARK Diagnostics, Inc.

发明人： Johnny Valdez ,  Byung Sook Moon ,  Alejandro A. Orozco ,  Gonzalo Naranjo

IPC分类号： G01N33/94 ,  C07C229/04 ,  A61K39/00 ,  G01N33/58 ,  C07C237/22 ,  C12N9/04 ,  C12N9/96 ,  C07K16/44

CPC分类号： G01N33/9406 ,  A61K39/00 ,  C07C229/04 ,  C07C237/22 ,  C07K16/44 ,  C07K2317/33 ,  C12N9/0006 ,  C12N9/96 ,  C12Y101/01049 ,  G01N33/581

摘要： Compounds and methods for use in detecting pregabalin in a sample suspected of containing pregabalin are disclosed. Pregabalin derivatives are described for producing pregabalin conjugates. A pregabalin-immunogenic carrier conjugate may be used as an immunogen for the preparation of an anti-pregabalin antibody. A pregabalin-detectable label conjugate may be used in a signal producing system in pregabalin assays.

公开(公告)号：US10072276B2

公开(公告)日：2018-09-11

申请号：US15066775

申请日：2016-03-10

申请人： SAMSUNG ELECTRONICS CO., LTD.

发明人： Yukyung Jung ,  Kwangmyung Cho ,  Jinha Kim ,  Soonchun Chung

IPC分类号： C12N15/52 ,  C12P7/56 ,  C12N9/12 ,  C12N9/02 ,  C12N9/04

CPC分类号： C12P7/56 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1205 ,  C12N15/52 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y101/01049 ,  C12Y102/0101 ,  C12Y102/01012 ,  C12Y106/01001 ,  C12Y207/01023 ,  C12Y207/01086

摘要： Provided is a genetically engineered yeast cell having increased NADPH production, a method of increasing a NADPH level in a yeast cell, a method of preparing the genetically engineered yeast cell, and a method of producing lactate using the genetically engineered yeast cell.

公开(公告)号：US09575056B2

公开(公告)日：2017-02-21

申请号：US13554417

申请日：2012-07-20

申请人： Hyeon Suk Kim ,  Tae-Hee Koo ,  Young Ho Choi

发明人： Hyeon Suk Kim ,  Tae-Hee Koo ,  Young Ho Choi

IPC分类号： G01N33/52

CPC分类号： G01N33/526 ,  C12Q1/32 ,  C12Y101/01027 ,  C12Y101/01049 ,  G01N33/523 ,  G01N33/98 ,  G01N2333/90209 ,  G01N2333/904

摘要： The present invention relates to a strip for an improved lateral flow assay of a biological sample on a single plane and a lateral flow chromatography assay using a test device containing the same. The strip of the present invention consists of a single-pad, which can improve lateral flow assay by providing an easy and simple procedure and clear visual reading. The strip of the present invention is consisted of sample application (sample) zone and reactant-resultant zone where the reaction mixture is deposited (reactant) are all on a same plane. In addition, the present invention provides a chromatographic method wherein hemoglobin is separated from analyte by a differential chromatography on the solid phase. Any interference of detection of the result by hemoglobin is removed by the present invention. The present invention provides advantages including an easy and simple procedure with a quick and clear response.

摘要翻译： 本发明涉及一种用于在单个平面上生物样品的改进的侧向流动测定的条带和使用含有该条带的测试装置的侧向流色谱分析。 本发明的条带由单垫组成，其可以通过提供简单且简单的程序和清晰的视觉读数来改善侧向流动测定。 本发明的条带由反应混合物沉积（反应物）的样品施加（样品）区域和反应物生成区域都在同一平面上。 此外，本发明提供了一种色谱法，其中通过固相上的差示色谱法将血红蛋白与分析物分离。 通过本发明除去血红蛋白检测结果的任何干扰。 本发明提供了包括具有快速和明确响应的简单且简单的程序的优点。

公开(公告)号：US20160326556A1

公开(公告)日：2016-11-10

申请号：US14891622

申请日：2015-02-04

申请人： INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES

发明人： Tingyi Wen ,  Xiuling Shang ,  Yun Zhang ,  Shuwen Liu ,  Yong Liang ,  Yu Zhang

IPC分类号： C12P13/24 ,  C12N9/92 ,  C12N15/77 ,  C12N9/04

CPC分类号： C12P13/24 ,  C12N9/0006 ,  C12N9/92 ,  C12N15/77 ,  C12Y101/01043 ,  C12Y101/01049 ,  C12Y503/01009

摘要： The present application discloses recombinant bacteria producing L-amino acid(s) its construction method and the method of producing L-amino acid(s). The recombinant bacteria producing L-amino acid(s) according to the present invention has reduced expression of the glucose-6-phosphate isomerase Pgi and improved expression of the glucose-6-phosphate dehydrogenase Zwf-OpcA than the starting bacteria, wherein: said starting bacterium is a bacterial strain which can accumulate target amino acid(s). During fermenting and culturing the recombinant bacteria according to the present invention, it is observed that the effect of improving yield can be additive and the yield of L-amino acid(s) is improved obviously. The strategy of combinational modification according to the present invention develops a new method of improving the yield of L-amino acid(s) and hence it can be applied to produce L-amino acid(s) through bacterialfermentation.

摘要翻译： 本申请公开了产生L-氨基酸的重组细菌的构建方法和L-氨基酸的生产方法。 根据本发明的产生L-氨基酸的重组细菌比起始细菌具有降低葡萄糖-6-磷酸异构酶Pgi的表达和改善葡萄糖-6-磷酸脱氢酶Zwf-OpcA的表达，其中：所述 起始细菌是能够积聚目标氨基酸的细菌菌株。 在根据本发明的重组细菌的发酵和培养过程中，观察到提高产量的效果可以是添加剂，并且L-氨基酸的产率显着提高。 根据本发明的组合修饰的策略开发出一种提高L-氨基酸产率的新方法，因此可用于通过细菌发酵产生L-氨基酸。