公开(公告)号：US09759551B2

公开(公告)日：2017-09-12

申请号：US14913385

申请日：2014-08-22

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Falk Schlaudraff ,  Qing Tang

IPC: G01B11/14 ,  G02B21/32 ,  G01N33/483 ,  G06T7/00 ,  G01N1/28

CPC classification number: G01B11/14 ,  G01N33/4833 ,  G01N2001/282 ,  G01N2001/2886 ,  G02B21/32 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30204

Abstract: A method for calibrating a laser deflection device in a reflected light device of a microscope of a laser microdissection system having a digital image capturing unit comprising an image evaluation module includes generating a laser beam; guiding the laser beam through a microscope objective; directing the laser beam to a position defined by actuation signals; placing a calibration object in the object plane of the microscope objective; actuating the laser deflection device using first actuation signals and first calibration values, making at least one calibration mark on the calibration object; capturing an image of the calibration object by the digital image capturing unit; determining actual position values for the at least one calibration mark: and determining second calibration values based on a relationship between the default position values and the actual position values.

公开(公告)号：US09335534B2

公开(公告)日：2016-05-10

申请号：US13969910

申请日：2013-08-19

Applicant: Leica Microsystems CMS GmbH

Inventor： Falk Schlaudraff

IPC: G02B21/34 ,  G03F7/20 ,  G02B21/36

CPC classification number: G02B21/34 ,  G01N2001/282 ,  G02B21/36 ,  G03F7/20

Abstract: A specimen slide (100) having a sample region (101) in which a sample (200) that can be investigated microscopically can be arranged, and that comprises reference points (A-Z, 1-24, a-j, α-κ) arranged at least in the sample region (101), is proposed. The reference points (A-Z, 1-24, a-j, α-κ) are embodied in such a way that on the basis of an identification of at least a stipulated number of reference points (A-Z, 1-24, a-j, α-κ) in an arbitrary sub-region (102) of the sample region (101), the position of the arbitrary sub-region (102), and/or at least one position therein, on the specimen slide (100) can be unequivocally determined A method for determining and/or retrieving a position on a corresponding specimen slide, and a corresponding sample investigation system, are likewise subjects of the invention.

Abstract translation: 具有样本区域（101）的标本片（100），其中可以布置可以显微镜检查的样本（200），并且包括参考点（AZ，1-24，aj，α-＆kgr），其布置在 至少在样本区域（101）中。 参考点（AZ，1-24，aj，α-＆kgr）以这样的方式实现：基于至少指定参考点数目（AZ，1-24，aj， 在样品区域（101）的任意子区域（102）中，任意子区域（102）的位置和/或其中的至少一个位置在样品载玻片（100）上可以是 明确确定用于确定和/或检索相应标本载片上的位置的方法和相应的样本调查系统也是本发明的主题。

公开(公告)号：US20140098214A1

公开(公告)日：2014-04-10

申请号：US14049241

申请日：2013-10-09

Applicant: Leica Microsystems CMS GmbH

Inventor： Falk Schlaudraff ,  Florian Hoffmann

IPC: G01N33/483

CPC classification number: G01N33/483 ,  G01N1/2813 ,  G01N2001/282 ,  G01N2001/2886

Abstract: A method for laser microdissection of a laser microdissection region of a prepared specimen includes driving a holder for the specimen into a holding position using a control device. First and second digital images are captured that depict a same portion of the prepared specimen, with the first image depicting the portion under at least one first microscopic examination method and the second image depicting the portion under at least a second microscopic examination method. A live overlay image is generated of the portion of the prepared image in a live mode. The live overlay is presented on a display area with the images overlaid onto one another. A marking is generated and captured on the live overlay image so as to define the laser microdissection region.

Abstract translation: 用于制备样品的激光显微切割区域的激光显微切割的方法包括使用控制装置将样品的保持器驱动到保持位置。 捕获第一和第二数字图像，其描绘所制备的样本的相同部分，其中第一图像在至少一个第一显微镜检查方法下描绘该部分，而第二图像在至少第二显微镜检查方法下描绘该部分。 在实时模式中，制作的图像的部分生成实时叠加图像。 实时叠加显示在显示区域上，图像叠加在一起。 在活动叠加图像上生成并捕获标记，以便定义激光显微切割区域。