公开(公告)号：US20160296242A1

公开(公告)日：2016-10-13

申请号：US15096256

申请日：2016-04-11

Applicant: Massachusetts Institute of Technology

Inventor： Nikita Pak ,  Justin P. Kinney ,  Edward S. Boyden

IPC: A61B17/16 ,  A61B34/32 ,  A61B90/10

Abstract: A method for automated opening of craniotomies includes, under the control of a computer processor, drilling into a skull for a predetermined distance and determining when there is a conductance drop near the drilling tip that indicates skull breakthrough. If the conductance is not below a predetermined threshold, drilling continues iteratively manner until conductance is below the threshold. A craniotomy pattern may be predetermined and automatically drilled under control of the processor. A cranial window may be created by drilling along a path that interpolates between holes to form the circumference of the window. An automated craniotomy opening apparatus includes a drilling apparatus with a drilling tip, at least one drilling apparatus positioning device, a detection device, and a computer processor that controls the drilling apparatus, the positioning device, and the detection device according to the method. Determining conductance may include use of an impedance detection circuit.

Abstract translation: 开颅手术自动打开的方法包括在计算机处理器的控制下，将颅骨钻入预定距离，并确定何时在钻尖附近有指示颅骨突破的电导滴落。 如果电导率不低于预定阈值，则钻探继续迭代，直到电导低于阈值。 开颅模式可以在处理器的控制下被预先确定并自动钻孔。 可以通过在孔之间内插的路径钻孔来形成颅窗，以形成窗的圆周。 自动开颅开放装置包括具有钻头的钻孔装置，至少一个钻孔装置定位装置，检测装置以及根据该方法控制钻孔装置，定位装置和检测装置的计算机处理器。 确定电导可以包括使用阻抗检测电路。

公开(公告)号：US20240287627A1

公开(公告)日：2024-08-29

申请号：US18588779

申请日：2024-02-27

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： Yong Qian ,  Edward S. Boyden

IPC: C12Q1/6897 ,  A61B5/00 ,  C12N15/85

CPC classification number: C12Q1/6897 ,  C12N15/85 ,  A61B5/0071

Abstract: The invention, in some aspects, include methods and systems for temporally multiplexed imaging (TMI).

公开(公告)号：US20220112553A1

公开(公告)日：2022-04-14

申请号：US17500060

申请日：2021-10-13

Applicant: Massachusetts Institute of Technology ,  The Broad Institute, Inc.

Inventor： Edward S. Boyden ,  Fei Chen ,  Nikita Obidin ,  Andrew Colin Payne

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: Methods for single-molecule analysis of structure and sequence of linearized polynucleotides are provided.

公开(公告)号：US20210298769A1

公开(公告)日：2021-09-30

申请号：US17088494

申请日：2020-11-03

Applicant: Massachusetts Institute of Technology

Inventor： Nikita Pak ,  Justin P. Kinney ,  Edward S. Boyden

IPC: A61B17/16 ,  A61B34/30 ,  A61B34/32 ,  A61B90/10

Abstract: An automated craniotomy opening apparatus includes a drilling apparatus with a drilling tip, at least one drilling apparatus positioning device, a detection device, and a computer processor that automatically controls the drilling apparatus, the positioning device, and the detection device. A method for automated opening of craniotomies includes, under automatice control of a computer processor, drilling into a skull for a predetermined distance and determining when there is a conductance drop near the drilling tip that indicates skull breakthrough. If the conductance is not below a predetermined threshold, drilling continues iteratively manner until conductance is below the threshold. A craniotomy pattern may be predetermined and automatically drilled under control of the processor. A cranial window may be created by drilling along a path that interpolates between holes to form the circumference of the window. Determining conductance may include use of an impedance detection circuit.

公开(公告)号：US10683510B2

公开(公告)日：2020-06-16

申请号：US15430502

申请日：2017-02-12

Applicant: Massachusetts Institute of Technology

Inventor： Edward S. Boyden ,  Katarzyna P. Adamala ,  Daniel A. Martin-Alarcon

IPC: C12N15/63

Abstract: The invention, in some aspects relates to compositions comprising synthetic minimal cells (SMCs) and use of SMCs, pluralities of SMCs in relation to engineering genetic circuit interactions.

公开(公告)号：US20180028081A1

公开(公告)日：2018-02-01

申请号：US15643462

申请日：2017-07-06

Applicant: Massachusetts Institute of Technology ,  Georgia Tech Research Corporation

Inventor： Ho-Jun Suk ,  Edward S. Boyden ,  Ingrid van Welie ,  Brian Douglas Allen ,  Suhasa B. Kodandaramaiah ,  Craig R. Forest

IPC: A61B5/04 ,  A61B5/00 ,  A61B34/32 ,  A61B5/053

CPC classification number: A61B5/04001 ,  A61B5/0071 ,  A61B5/0538 ,  A61B5/4064 ,  A61B5/6885 ,  A61B34/32 ,  A61B2503/40

Abstract: In an automated methodology for in vivo image-guided cell patch clamping, a cell patch clamping device is moved into position and targeted to a specific cell using automated image-guided techniques. Cell contact is determined by analyzing the temporal series of measured resistance levels at the clamping device as it is moved. The difference between successive resistance levels is compared to a threshold, which must be exceeded before cell contact is assumed. Pneumatic control methods are used to achieve gigaseal formation and cell break-in, leading to whole-cell patch clamp formation. An automated robotic system capable of performing this methodology automatically performs patch clamping in vivo, automatically locating cells through image guidance and by analyzing the temporal sequence of electrode impedance changes. By continuously monitoring the patching process and rapidly executing actions triggered by specific measurements, the robot can rapidly find target cells in vivo and establish patch-clamp recordings.