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公开(公告)号：US10993634B2
公开(公告)日：2021-05-04
申请号：US15643462
申请日：2017-07-06
Inventor： Ho-Jun Suk , Edward S. Boyden , Ingrid van Welie , Brian Douglas Allen , Suhasa B. Kodandaramaiah , Craig R. Forest
IPC: G06K9/00 , A61B5/04 , A61B5/0538 , A61B34/32 , A61B5/00
Abstract: In an automated methodology for in vivo image-guided cell patch clamping, a cell patch clamping device is moved into position and targeted to a specific cell using automated image-guided techniques. Cell contact is determined by analyzing the temporal series of measured resistance levels at the clamping device as it is moved. The difference between successive resistance levels is compared to a threshold, which must be exceeded before cell contact is assumed. Pneumatic control methods are used to achieve gigaseal formation and cell break-in, leading to whole-cell patch clamp formation. An automated robotic system capable of performing this methodology automatically performs patch clamping in vivo, automatically locating cells through image guidance and by analyzing the temporal sequence of electrode impedance changes. By continuously monitoring the patching process and rapidly executing actions triggered by specific measurements, the robot can rapidly find target cells in vivo and establish patch-clamp recordings.
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公开(公告)号：US10590181B2
公开(公告)日：2020-03-17
申请号：US14653040
申请日：2015-04-18
Applicant: Massachusetts Institute of Technology
Inventor： Edward S. Boyden , Yongku Peter Cho , Nathan C. Klapoetke , Amy S. Chuong , Fei Chen
IPC: C07K14/705 , C07K14/405 , G01N33/50 , A61K38/00
Abstract: The invention, in some aspects relates to compositions and methods for altering cell activity and function and the introduction and use of mutant light-activated ion channels and variants thereof.
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3.发明申请公开(公告)号：US20160238593A1
公开(公告)日：2016-08-18
申请号：US14995169
申请日：2016-01-13
Applicant: Massachusetts Institute of Technology
IPC: G01N33/53
CPC classification number: G01N33/5308 , C07K14/47 , C40B40/10
Abstract: A programmable modular protein architecture for RNA binding comprises a set of modules, derived from RNA-binding protein Pumilio, that can be concatenated into chains of varying composition and length. When bound into a chain, each module has a preferred affinity for a specific RNA base. The chains can bind arbitrary RNA sequences with high specificity and fidelity by varying the sequence of modules within the chains. Each module contains at least 6 amino acids, with the amino acids in positions 1 and 5 providing the preferred affinity for the specific base, and the amino acid at position 2 serving as a stacking unit between concatenated modules. The modules may have four canonic forms, each having a preferred affinity for a different base and characterized by the base with which it has affinity, the two amino acids that provide the affinity, and the amino acid that serves as a stacking unit.
Abstract translation: 用于RNA结合的可编程模块化蛋白质结构包括衍生自RNA结合蛋白Pumilio的一组模块，其可以连接成不同组成和长度的链。 当结合到链中时，每个模块对于特定的RNA碱基具有优选的亲合力。 链可以通过改变链内的模块序列，以高特异性和保真度结合任意的RNA序列。 每个模块含有至少6个氨基酸，位置1和5中的氨基酸对特定碱提供优选的亲和力，而位置2上的氨基酸用作连接模块之间的堆叠单元。 模块可以具有四种正方形，每种具有对不同碱基的优选亲和力，其特征在于具有亲和力的碱，提供亲和力的两个氨基酸和用作堆积单元的氨基酸。
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公开(公告)号：US11564701B2
公开(公告)日：2023-01-31
申请号：US17088494
申请日：2020-11-03
Applicant: Massachusetts Institute of Technology
Inventor： Nikita Pak , Justin P. Kinney , Edward S. Boyden
Abstract: An automated craniotomy opening apparatus includes a drilling apparatus with a drilling tip, at least one drilling apparatus positioning device, a detection device, and a computer processor that automatically controls the drilling apparatus, the positioning device, and the detection device. A method for automated opening of craniotomies includes, under automatic control of a computer processor, drilling into a skull for a predetermined distance and determining when there is a conductance drop near the drilling tip that indicates skull breakthrough. If the conductance is not below a predetermined threshold, drilling continues iteratively manner until conductance is below the threshold. A craniotomy pattern may be predetermined and automatically drilled under control of the processor. A cranial window may be created by drilling along a path that interpolates between holes to form the circumference of the window. Determining conductance may include use of an impedance detection circuit.
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公开(公告)号：US20170081489A1
公开(公告)日：2017-03-23
申请号：US15261163
申请日：2016-09-09
Applicant: Massachusetts Institute of Technology
Inventor： Samuel G. Rodriques , Daniel Oran , Ruixuan Gao , Shoh Asano , Mark A. Skylar-Scott , Fei Chen , Paul W. Tillberg , Adam H. Marblestone , Edward S. Boyden
CPC classification number: C08J7/123 , B29C35/0805 , B29C2035/0838 , B29K2105/0061 , B33Y80/00 , C08J3/075 , C08J3/28 , C08J7/02 , C08J7/12 , C08J2333/26
Abstract: The present invention enables three-dimensional nanofabrication by isotropic shrinking of patterned hydrogels. A hydrogel is first expanded, the rate of expansion being controlled by the concentration of the crosslinker. The hydrogel is then infused with a reactive group and patterned in three dimensions using a photon beam through a limited-diffraction microscope. Functional particles or materials are then deposited on the pattern. The hydrogel is then shrunk and cleaved from the pattern.
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Patent Agency Ranking
公开(公告)号：US20230250139A1
公开(公告)日：2023-08-10
申请号：US18045624
申请日：2022-10-11
Applicant: Massachusetts Institute of Technology
Inventor： Changyang Linghu , Edward S. Boyden
IPC: C07K14/245 , G01N33/50 , C12N15/63
CPC classification number: C07K14/245 , G01N33/5023 , C12N15/63 , C07K2319/24 , C07K2319/735
Abstract: The invention, in some aspects, includes compositions encoding expression-recording islands (XRIs), compositions comprising XRIs, and methods for using XRIs for intracellular molecular recording.
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公开(公告)号：US11275081B2
公开(公告)日：2022-03-15
申请号：US16452362
申请日：2019-06-25
Applicant: Massachusetts Institute of Technology
Abstract: A programmable modular protein architecture for RNA binding comprises a set of modules, derived from RNA-binding protein Pumilio, that can be concatenated into chains of varying composition and length. When bound into a chain, each module has a preferred affinity for a specific RNA base. The chains can bind arbitrary RNA sequences with high specificity and fidelity by varying the sequence of modules within the chains. Each module contains at least 6 amino acids, with the amino acids in positions 1 and 5 providing the preferred affinity for the specific base, and the amino acid at position 2 serving as a stacking unit between concatenated modules. The modules may have four canonic forms, each having a preferred affinity for a different base and characterized by the base with which it has affinity, the two amino acids that provide the affinity, and the amino acid that serves as a stacking unit.
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公开(公告)号：US10330674B2
公开(公告)日：2019-06-25
申请号：US14995169
申请日：2016-01-13
Applicant: Massachusetts Institute of Technology
Abstract: A programmable modular protein architecture for RNA binding comprises a set of modules, derived from RNA-binding protein Pumilio, that can be concatenated into chains of varying composition and length. When bound into a chain, each module has a preferred affinity for a specific RNA base. The chains can bind arbitrary RNA sequences with high specificity and fidelity by varying the sequence of modules within the chains. Each module contains at least 6 amino acids, with the amino acids in positions 1 and 5 providing the preferred affinity for the specific base, and the amino acid at position 2 serving as a stacking unit between concatenated modules. The modules may have four canonic forms, each having a preferred affinity for a different base and characterized by the base with which it has affinity, the two amino acids that provide the affinity, and the amino acid that serves as a stacking unit.
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公开(公告)号：US10048255B2
公开(公告)日：2018-08-14
申请号：US14995169
申请日：2016-01-13
Applicant: Massachusetts Institute of Technology
Abstract: A programmable modular protein architecture for RNA binding comprises a set of modules, derived from RNA-binding protein Pumilio, that can be concatenated into chains of varying composition and length. When bound into a chain, each module has a preferred affinity for a specific RNA base. The chains can bind arbitrary RNA sequences with high specificity and fidelity by varying the sequence of modules within the chains. Each module contains at least 6 amino acids, with the amino acids in positions 1 and 5 providing the preferred affinity for the specific base, and the amino acid at position 2 serving as a stacking unit between concatenated modules. The modules may have four canonic forms, each having a preferred affinity for a different base and characterized by the base with which it has affinity, the two amino acids that provide the affinity, and the amino acid that serves as a stacking unit.
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公开(公告)号：US20170320039A1
公开(公告)日：2017-11-09
申请号：US15586246
申请日：2017-05-03
Applicant: Massachusetts Institute of Technology
Inventor： Ruixuan Gao , Ishan Gupta , Edward S. Boyden
Abstract: A method for synthesizing nanoparticles by sonofragmentation includes dispersing ultra-thin substrate units in a solvent chosen for suitability for sonofragmentation of the substrate, forming a suspension; ultrasonicating the suspension for a length of time sufficient to fragment the substrate into nanoparticles that are dispersed in the solvent; and evaporating the solvent. Solvent exchange with a second solvent may be performed. The synthesized nanoparticles are highly crystalline and monodispersed. The surface of the synthesized nanoparticles may be functionalized by choosing the solvents according to chemistry related to the intended surface functionalization of the synthesized nanoparticles, by adding surfactants to one or more of the solvents, and/or by performing ligand exchange or chemical modification to replace surface-bonded solvent or surfactant molecules with other functional groups to produce nanoparticles having the desired surface functionalization.
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