公开(公告)号：US20190390182A1

公开(公告)日：2019-12-26

申请号：US16540180

申请日：2019-08-14

Applicant: NOVOZYMES A/S

Inventor： Rakhi Saikia ,  Geetha Hiremath Mendez ,  Sohel Dalal ,  Padmavathi Balumuri ,  Santhosh Vasu Mepadam ,  Lone Baunsgaard ,  Jesper Vind ,  Garry Paul Gippert ,  Kim Borch

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10023852B2

公开(公告)日：2018-07-17

申请号：US15310128

申请日：2015-05-26

Applicant: Novozymes A/S

Inventor： Padmavathi Balumuri ,  Rakhi Saikia ,  Allan Svendsen ,  Lone Baunsgaard

IPC: C12N9/20 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C11D3/386 ,  C12P21/06 ,  C12P21/04 ,  C12N15/09 ,  C12P7/64

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20150093795A1

公开(公告)日：2015-04-02

申请号：US14400090

申请日：2013-04-30

Applicant: NOVOZYMES A/S

Inventor： Aditya Basu ,  Naik Sangeeta ,  Santhosh Mepadam Vasu ,  Paul Pritish ,  Rakhi Saikia ,  Allan Svendsen

IPC: C12P7/42 ,  C12N9/18

CPC classification number: C12P7/42 ,  C08J11/105 ,  C12N9/18 ,  C12Y301/01074 ,  Y02P20/52

Abstract: The present invention relates tocutinasevariants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及角质酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US11492605B2

公开(公告)日：2022-11-08

申请号：US16605086

申请日：2018-04-26

Applicant: Novozymes A/S

Inventor： Vivek Srivastava ,  Markus Klinger ,  Rakhi Saikia ,  Vijaya Shankar Nataraj ,  Sohel Dalal ,  Jens Erik Nielsen ,  Rajendra Kulothungan Sainathan

IPC: C12N9/42 ,  C11D3/386

Abstract: The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US11326153B2

公开(公告)日：2022-05-10

申请号：US16770797

申请日：2018-12-07

Applicant: NOVOZYMES A/S

Inventor： Carsten Andersen ,  Vasudeva Prahlada Rao ,  Rakhi Saikia ,  Vivek Srivastava ,  Kendra Darlene Stallings

IPC: C12N9/28 ,  C12P19/02 ,  C12P19/14 ,  C13K1/06

Abstract: The present invention relates to alpha-amylase variants comprising a substitution at a position corresponding to position 188 and at least one further substitution at a position corresponding to position 242 or 279 or 275 of SEQ ID NO: 1, in particular one or more combinations of substitutions selected from the group consisting of E188P+S242Y, E188P+S242F, E188P+S242H, E188P+S242W, E188P+S242P, E188P+S242I, E188P+S242T, E188P+S242L, E188P+K279W, E188P+K279Y, E188P+K279F, E188P+K279H, E188P+K279I, E188P+K279L, E188P+K279D, E188P+K279M, E188P+K279S, E188P+K279T E188P+K279N, E188P+K279Q, E188P+K279V, E188P+K279A, E188P+N275F, E188P+N275Y, E188P+N275W, and E188P+N275H, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, and SEQ ID NO: 27. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10822598B2

公开(公告)日：2020-11-03

申请号：US15742095

申请日：2016-07-06

Applicant: NOVOZYMES A/S

Inventor： Rakhi Saikia ,  Geetha Hiremath Mendez ,  Sohel Dalal ,  Padmavathi Balmuri ,  Santhosh Vasu Mepadam ,  Lone Baunsgaard ,  Jesper Vind ,  Garry Paul Gippert ,  Kim Borch

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 5 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 10 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20200255812A1

公开(公告)日：2020-08-13

申请号：US16865744

申请日：2020-05-04

Applicant: Novozymes A/S

Inventor： Allan Svendsen ,  Rakhi Saikia ,  Sohel Dalal ,  Vikram Kjoeller Bhatia ,  Lone Baunsgaard

IPC: C12N9/20

Abstract: The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence

公开(公告)号：US20190338260A1

公开(公告)日：2019-11-07

申请号：US16439912

申请日：2019-06-13

Applicant: NOVOZYMES A/S

Inventor： Padmavathi Balumuri ,  Rakhi Saikia ,  Allan Svendsen ,  Lone Baunsgaard

IPC: C12N9/20 ,  C12P7/64 ,  C12P21/06 ,  C11D3/386 ,  C12N15/09

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20180371438A1

公开(公告)日：2018-12-27

申请号：US15742095

申请日：2016-07-06

Applicant: NOVOZYMES A/S

Inventor： Rakhi Saikia ,  Geetha Hiremath Mendez ,  Sohel Dalal ,  Padmavathi Balmuri ,  Santhosh Vasu Mepadam ,  Lone Baunsgaard ,  Jesper Vind ,  Garry Paul Gippert ,  Kim Borch

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 5 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 10 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20180340159A1

公开(公告)日：2018-11-29

申请号：US15778330

申请日：2016-11-30

Applicant: NOVOZYMES A/S

Inventor： Lone Baunsgaard ,  Allan Svendsen ,  Kim Borch ,  Rakhi Saikia ,  Jakob Skjold-Jørgensen

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention relates to a method for generating a preparation of a lipase variant of a parent lipase comprising a step of altering one or more nucleotides in a polynucleotide encoding the parent lipase, wherein the alteration provides at least one disulfide bond and a hydrolytic activity of said lipase variant which is reduced as compared with the hydrolytic activity of the parent lipase. The invention also relates to a polynucleotide encoding the lipase variant, the lipase variant and its use.