公开(公告)号：US12116601B2

公开(公告)日：2024-10-15

申请号：US17700598

申请日：2022-03-22

Applicant: Novozymes A/S

Inventor： Marco Malten ,  Kim Borch ,  Lise Munch Mikkelsen ,  Thomas Agersten Poulsen ,  Luise Erlandsen ,  Hanne Philbert Nielsen ,  Carsten Horslev Hansen ,  Allan Svendsen ,  Christian Lundager Gylstorff ,  Jesper Vind ,  Christian Jackel

IPC: C12N9/18 ,  A61K8/66 ,  B65D65/46 ,  C11D3/386 ,  C11D17/04 ,  C12N9/20

CPC classification number: C12N9/18 ,  A61K8/66 ,  B65D65/46 ,  C11D3/38627 ,  C11D17/042 ,  C11D17/043 ,  C12N9/20 ,  C12Y301/01003 ,  C12Y301/01

Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.

公开(公告)号：US20230295591A1

公开(公告)日：2023-09-21

申请号：US18152992

申请日：2023-01-11

Applicant: Novozymes A/S

Inventor： Svend Gunnar Kaasgaard ,  Frank Winther Rasmussen ,  Roland Alexander Pache ,  Lars Anderson ,  Mette Louise Dissing Overgaard ,  Thomas Agersten Poulsen ,  Dorte Marie Koefoed Klitgaard ,  Christian Isak Joergensen ,  Lars Giger

IPC: C12N9/42 ,  C11D3/386

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004

Abstract: Disclosed is variants of a cellulase having improved stability in the presence of a protease, and the use of such variants in laundry.

公开(公告)号：US11603522B2

公开(公告)日：2023-03-14

申请号：US16715041

申请日：2019-12-16

Applicant: NOVOZYMES A/S ,  NOVOZYMES NORTH AMERICA, INC.

Inventor： Carsten Andersen ,  Randall Deinhammer ,  Thomas Agersten Poulsen ,  Miguel Duarte Toscano ,  Peter Kamp Hansen ,  Henrik Friis-Madsen ,  Anders Viksoe-Nielsen ,  Signe Eskildsen Larsen ,  Lars L. H. Christensen

IPC: C12N9/28 ,  C11D3/386 ,  C12P7/06 ,  C12P19/04 ,  C12P19/14 ,  C12N9/26 ,  C12P7/14 ,  C12P19/02 ,  C12N15/82

Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.

公开(公告)号：US20220213453A1

公开(公告)日：2022-07-07

申请号：US17700598

申请日：2022-03-22

Applicant: Novozymes A/S

Inventor： Marco Malten ,  Kim Borch ,  Lise Munch Mikkelsen ,  Thomas Agersten Poulsen ,  Luise Erlandsen ,  Hanne Philbert Nielsen ,  Carsten Horslev Hansen ,  Allan Svendsen ,  Christian Lundager Gylstorff ,  Jesper Vind ,  Christian Jackel

IPC: C12N9/18 ,  C12N9/20 ,  C11D17/04 ,  C11D3/386 ,  B65D65/46 ,  A61K8/66

Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning.
The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.

公开(公告)号：US11268076B2

公开(公告)日：2022-03-08

申请号：US15526834

申请日：2015-12-08

Applicant: Novozymes A/S

Inventor： Christian Lundager Gylstorff ,  Christian Jackel ,  Jesper Vind ,  Allan Svendsen ,  Marco Malten ,  Thomas Agersten Poulsen

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention relates to a variant of a parent lipase, which parent lipase has the amino acid sequence of SEQ ID NO: 2, wherein the variant has lipase activity, comprises a substitution at a position corresponding to position 51 and comprises a substitution or remains unaltered at a position corresponding to position 96 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US11203790B2

公开(公告)日：2021-12-21

申请号：US16656708

申请日：2019-10-18

Applicant: NOVOZYMES A/S

Inventor： Shikha Gupta ,  Allan Svendsen ,  Vivek Srivastava ,  Marco Malten ,  Thomas Agersten Poulsen ,  Preethi Ramaiya ,  Beth Nelson ,  Vasudeva Prahlada Rao ,  Padmavathi Balumuri

IPC: C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  C12N9/34

Abstract: The present invention relates to glucoamylase variants, polynucleotides encoding the variants, methods of producing the variants, and methods of using the variants. Also described are the use of glucoamylases of the invention for starch conversion to produce fermentation products, such as ethanol, and syrups, such as glucose. The invention also relates to a composition comprising a glucoamylase variant of the invention.

公开(公告)号：US20210071157A1

公开(公告)日：2021-03-11

申请号：US16962425

申请日：2019-02-08

Applicant: Novozymes A/S

Inventor： Sune Fang Christensen ,  Allan Svendsen ,  Jesper Vind ,  Anders Gunnar Sandtrom ,  Thomas Agersten Poulsen ,  Alexander David Fulton ,  Carsten Hoerslev Hansen ,  Vibeke Skovgaard Nielsen ,  Kim Borch ,  Garry Paul Gippert

IPC: C12N9/20 ,  C07K1/12 ,  C11D3/386

Abstract: The present invention relates to lipase variants of the parent lipase shown as SEQ ID NO: 2 with improved wash performance and/or Benefit Risk Factor (BRF). The invention also relates to polynucleotides encoding the lipase variants of the invention, compositions comprising a lipase variant of the invention as well as methods of producing such lipase variants of the invention and the use of the lipase variants or compositions thereof.

公开(公告)号：US20190048413A1

公开(公告)日：2019-02-14

申请号：US16079141

申请日：2017-02-23

Applicant: NOVOZYMES A/S

Inventor： Marco Malten ,  Thomas Agersten Poulsen ,  Preethi Ramaiya ,  Michael Bargisen ,  Beth Nelson

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876

Abstract: The present invention enables the determination by NGS of the full nucleotide sequence of one or more polynucleotides of interest that are more than 800 bp in length after only one round of PCR amplification and subsequent fragmentation by adding at least one known oligonucleotide index to at least one end of each polynucleotide to produce elongated polynucleotides, wherein each known oligonucleotide index added to each polynucleotide is unique; fragmenting the elongated polynucleotides; sequencing the resulting fragments with paired forward and reverse reads using bridge amplification and next-generation sequencing; sorting the paired sequences based on the known oligonucleotide; and performing manual or in silico assembly of the sorted paired sequences, thereby providing the full coding sequence of each of the multitude of polynucleotides.

公开(公告)号：US20170321197A1

公开(公告)日：2017-11-09

申请号：US15526834

申请日：2015-12-08

Applicant: Novozymes A/S

Inventor： Christian Lundager Gylstorff ,  Christian Jackel ,  Jesper Vind ,  Allan Svendsen ,  Marco Malten ,  Thomas Agersten Poulsen

IPC: C12N9/20 ,  C11D3/386

CPC classification number: C12N9/20 ,  C11D3/38627 ,  C12Y301/01

Abstract: The present invention relates to a variant of a parent lipase, which parent lipase has the amino acid sequence of SEQ ID NO: 2, wherein the variant has lipase activity, comprises a substitution at a position corresponding to position 51 and comprises a substitution or remains unaltered at a position corresponding to position 96 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US09139822B2

公开(公告)日：2015-09-22

申请号：US14064911

申请日：2013-10-28

Applicant: NOVOZYMES A/S

Inventor： Carsten Andersen ,  Thomas Agersten Poulsen

IPC: C12N9/26 ,  C12N9/28 ,  C11D3/386 ,  C12P7/06 ,  C12P19/04 ,  C12P19/14

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/2414 ,  C12N15/8257 ,  C12P7/06 ,  C12P7/14 ,  C12P19/02 ,  C12P19/04 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01001 ,  Y02E50/17

Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.

Abstract translation: 本发明涉及α-淀粉酶，编码α-淀粉酶的核酸，α-淀粉酶的生产方法和使用α-淀粉酶的方法。