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    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    3.
    发明公开
    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开

    公开(公告)号：US20230142991A1

    公开(公告)日：2023-05-11

    申请号：US17797351

    申请日：2021-02-09

    申请人： Novozymes A/S

    发明人： Carsten Andersen ,  Thomas Agersten Poulsen ,  Dorota Nissen ,  Marie Thryøse Kruse ,  Sarah Schultheis Elliott ,  Shiro Fukuyama

    IPC分类号： C12N9/28 ,  C12N9/34 ,  C12P7/06

    CPC分类号： C12N9/2417 ,  C12Y302/01001 ,  C12N9/2428 ,  C12P7/06

    摘要： The present invention relates to isolated alpha-amylase variants of a parent alpha-amylase, comprising an alteration at one or more positions corresponding to positions 196, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 28, 38, 39, 43, 54, 56, 57, 64, 67, 68, 70, 71, 86, 89, 90, 94, 96, 99, 101, 103, 107, 108, 110, 113, 114, 117, 127, 134, 138, 142, 150, 151, 152, 156, 169, 171, 174, 179, 183, 193, 199, 200, 204, 205, 207, 208, 209, 212, 218, 221, 222, 224, 233, 241, 245, 259, 275, 278, 281, 282, 283, 284, 285, 308, 323, 335, 348, 359, 382, 386, 388, 392, 394, 396, 412, 414, 417, 424, 428, 457, 459, 466, 479, 489, 511, 533, 534, 542, 543, 545, 547, 549, 550, 551, 560, 566, 570, 574, 575, 576, 577, 578, 580, 581, 582, 589, 592, 599, 603, 605, 608, 614, 619, or 626 of the polypeptide of SEQ ID NO: 1, wherein the variant has alpha-amylase activity and wherein the variant has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at 1.5 least 98%, or at least 99% sequence identity, but less than 100% sequence identity, to the polypeptide of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. The alpha-amylase variants of the invention have increased stability at pH 4.0 and 32° C. compared to the parent alpha-amylase.

    PROCESS FOR PRODUCING ETHANOL FROM RAW STARCH USING ALPHA-AMYLASE VARIANTS
    7.
    发明公开
    PROCESS FOR PRODUCING ETHANOL FROM RAW STARCH USING ALPHA-AMYLASE VARIANTS 审中-公开

    公开(公告)号：US20230235367A1

    公开(公告)日：2023-07-27

    申请号：US17798197

    申请日：2021-02-09

    申请人： Novozymes A/S

    发明人： Carsten Andersen ,  Thomas Agersten Poulsen ,  Dorota Nissen ,  Marie Thryøse Kruse ,  Sarah Schultheis Elliott ,  Shiro Fukuyama

    IPC分类号： C12P7/10 ,  C12N9/26 ,  C12N15/81 ,  C12N15/52

    CPC分类号： C12P7/10 ,  C12N9/2414 ,  C12N15/81 ,  C12N15/52 ,  C12Y302/01001

    摘要： The present invention relates to raw starch hydrolysis and in particular to a raw starch hydrolysis and fermentation process. More particularly the present invention relates to a process of producing a fermentation product from raw starch material, comprising the steps of: (a) saccharifying starch-containing material at a temperature below the initial gelatinization temperature of said starch-containing material; and (b) fermenting with a fermenting organism, wherein step (a) is carried out using at least a variant alpha-amylase comprising a substitution at one or more positions corresponding to positions 196, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 28, 38, 39, 43, 54, 56, 57, 64, 67, 68, 70, 71, 86, 89, 90, 94, 96, 99, 101, 103, 107, 108, 110, 113, 114, 117, 127, 134, 138, 142, 150, 151, 152, 156, 169, 171, 174, 179, 183, 193, 199, 200, 204, 205, 207, 208, 209, 212, 218, 221, 222, 224, 233, 241, 245, 259, 275, 278, 281, 282, 283, 284, 285, 308, 323, 335, 348, 359, 382, 386, 388, 392, 394, 396, 412, 414, 417, 424, 428, 457, 459, 466, 479, 489, 511, 533, 534, 542, 543, 545, 547, 549, 550, 551, 560, 566, 570, 574, 575, 576, 577, 578, 580, 581, 582, 589, 592, 599, 603, 605, 608, 614, 619, or 626 of the polypeptide of SEQ ID NO: 1, wherein the variant has alpha-amylase activity and wherein the variant has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity, but less than 100% sequence identity, to the polypeptide of SEQ ID NO: 1, and optionally a glucoamylase.