公开(公告)号：US20140147847A1

公开(公告)日：2014-05-29

申请号：US14168766

申请日：2014-01-30

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Wendy Yoder

IPC: C12N15/80 ,  C12P21/00

CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

公开(公告)号：US20220098631A1

公开(公告)日：2022-03-31

申请号：US17547836

申请日：2021-12-10

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Amanda Fischer ,  Suchindra Maiyuran

IPC: C12P19/14 ,  C12N9/42 ,  C07K14/38 ,  C12P19/02

Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

公开(公告)号：US11078509B2

公开(公告)日：2021-08-03

申请号：US16996159

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US11001869B2

公开(公告)日：2021-05-11

申请号：US16995820

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20200377921A1

公开(公告)日：2020-12-03

申请号：US16995820

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20190093143A1

公开(公告)日：2019-03-28

申请号：US16211632

申请日：2018-12-06

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/80 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20150225761A1

公开(公告)日：2015-08-13

申请号：US14663118

申请日：2015-03-19

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80

CPC classification number: C12P21/00 ,  C12N9/58 ,  C12N15/80 ,  C12P21/02

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

Abstract translation: 本发明涉及母体木霉菌株的突变体，其包含编码多肽的多核苷酸和选自第一枯草杆菌蛋白酶样丝氨酸蛋白酶基因，第一天冬氨酸蛋白酶基因，胰蛋白酶抑制蛋白酶基因的一个或多个（若干）基因， 如丝氨酸蛋白酶基因，第二枯草杆菌蛋白酶样丝氨酸蛋白酶基因和第二天冬氨酸蛋白酶基因，其中所述一个或多个（若干）基因被修饰，使得突变菌株缺乏产生一种或多种（若干）选自 与母本木霉属菌株相比，分别在相同的条件下培养时由第一枯草杆菌蛋白酶样丝氨酸蛋白酶，第一天冬氨酸蛋白酶，胰蛋白酶样丝氨酸蛋白酶，第二枯草杆菌蛋白酶样丝氨酸蛋白酶和第二天冬氨酸蛋白酶组成的组 条件。 本发明还涉及在这种突变体中产生多肽的方法和用于产生这种突变体的方法。