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    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain
    21.
    发明申请
    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain 审中-公开
    消除或减少丝状真菌菌株中基因表达的方法

    公开(公告)号：US20140287475A1

    公开(公告)日：2014-09-25

    申请号：US14255642

    申请日：2014-04-17

    Applicant: Novozymes A/S ,  Novozymes, Inc.

    Inventor： Howard Brody ,  Suchindra Maiyuran ,  Hiroaki Udagawa

    IPC: C12P1/02

    CPC classification number: C12P1/02 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

    Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.

    Abstract translation: 本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）将丝状真菌菌株的双链可转录核酸构建体插入基因组中，所述双链可转录核酸构建体包含第一核苷酸序列，其包含 可操作地连接到靶基因的同源编码区的启动子和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二核苷酸 序列相对于第一个核苷酸序列处于相反方向; 和（b）通过在有利于产生干扰RNA的条件下培养丝状真菌菌株诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。 本发明还涉及丝状真菌菌株以及在这种丝状真菌菌株中生产目的生物物质的方法。

    Methods for Producing Polypeptides in Protease-Deficient Mutants of Trichoderma
    23.
    发明申请
    Methods for Producing Polypeptides in Protease-Deficient Mutants of Trichoderma 有权

    公开(公告)号：US20210155967A1

    公开(公告)日：2021-05-27

    申请号：US17163033

    申请日：2021-01-29

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

    Methods for producing polypeptides in protease-deficient mutants of Trichoderma
    25.
    发明授权
    Methods for producing polypeptides in protease-deficient mutants of Trichoderma 有权

    公开(公告)号：US11078510B2

    公开(公告)日：2021-08-03

    申请号：US16996168

    申请日：2020-08-18

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

    Protease-deficient mutants of trichoderma for producing polypeptides
    26.
    发明授权
    Protease-deficient mutants of trichoderma for producing polypeptides 有权

    公开(公告)号：US10400260B2

    公开(公告)日：2019-09-03

    申请号：US14663118

    申请日：2015-03-19

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12N9/58 ,  C12P21/00 ,  C12N15/80 ,  C12P21/02

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

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