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    Mutated alkaline cellulase
    22.
    发明授权
    Mutated alkaline cellulase 有权
    突变碱性纤维素酶

    公开(公告)号：US09169458B2

    公开(公告)日：2015-10-27

    申请号：US13583508

    申请日：2011-03-11

    申请人： Ayako Shimazu ,  Keiji Endo ,  Mitsuyoshi Okuda

    发明人： Ayako Shimazu ,  Keiji Endo ,  Mitsuyoshi Okuda

    IPC分类号： C12N9/42 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C11D3/386

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.

    摘要翻译： 本发明提供具有增强的抗再沉积能力的突变型碱性纤维素酶。 本发明提供了一种具有氨基酸序列的突变型碱性纤维素酶，其中至少选自氨基酸残基的氨基酸残基位于对应于位置58,45,52,56,66,66,61,103,60,64,66,67,60,62,68,62,62,64,68,62,62,62,67,67,69,68,62,62,67 119,122,123,124,125,127,130,140,​​161,164,175,176,178,179,181,193,194,195,196,197,199,202,203,217,225， 227,228,251,267,272,276,277,280,282,277,310,312,318,324,345,354,356,357,360,363,368,418,419,420,421,418,417,318,472,427,268,267,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282 422,453,454,455,457,458,459,494,495,496,5500,501,502,503,504,550,551,552,604,605,606,607,608,640,641， 或SEQ ID NO：2所示的碱性纤维素酶的氨基酸序列的642,644,645,646,683,684,685,690,691,692,693,694,739,740和741，或 与SEQ ID NO：2的氨基酸序列具有至少70％同一性的碱性纤维素酶的氨基酸序列被其它氨基酸残基取代。

    Method of Producing Fatty Acids or Lipids Containing Fatty Acids Using Thioesterase Variants
    23.
    发明申请
    Method of Producing Fatty Acids or Lipids Containing Fatty Acids Using Thioesterase Variants 有权
    使用硫酯酶变体生产含有脂肪酸的脂肪酸或脂质的方法

    公开(公告)号：US20130219557A1

    公开(公告)日：2013-08-22

    申请号：US13516047

    申请日：2010-12-03

    申请人： Takuto Tojo ,  Keiji Endo

    发明人： Takuto Tojo ,  Keiji Endo

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8247 ,  C07K14/415 ,  C12N9/16 ,  C12N15/8218 ,  C12N15/8285 ,  C12P7/6463

    摘要： A method of producing a fatty acid or a lipid containing a fatty acid, using a thioesterase variant having an amino acid sequence in which the 231st amino acid is substituted from threonine to lysine in the amino acid sequence set forth in SEQ ID NO: 1; and a transformant obtained by introducing a gene that encodes the thioesterase variant which transformant has an enhanced ability to produce a fatty acid or a lipid containing a fatty acid.

    摘要翻译： 使用具有SEQ ID NO：1所示氨基酸序列中第231位氨基酸被苏氨酸取代为赖氨酸的氨基酸序列的硫酯酶变体的脂肪酸或含脂肪酸的脂质的制造方法; 以及通过引入编码转化体的硫酯酶变体的基因获得的转化体具有增强的产生脂肪酸的能力或含有脂肪酸的脂质的能力。

    Mutant bacterium belonging to the genus Bacillus
    25.
    发明授权
    Mutant bacterium belonging to the genus Bacillus 有权
    属于芽孢杆菌属的突变细菌

    公开(公告)号：US07855065B2

    公开(公告)日：2010-12-21

    申请号：US10590275

    申请日：2005-03-04

    申请人： Keiji Endo ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Katsuya Ozaki

    IPC分类号： C12N1/20 ,  C12N15/74 ,  C12N15/75 ,  C12P21/04

    CPC分类号： C12N9/2417 ,  C12N9/2437 ,  C12N9/54 ,  C12Y302/01004

    摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism. A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.

    摘要翻译： 本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。 一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物 通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。

    USE OF SULFONANILIDE COMPOUNDS AS HERBICIDE
    27.
    发明申请
    USE OF SULFONANILIDE COMPOUNDS AS HERBICIDE 审中-公开
    磺酰亚胺化合物用作除草剂

    公开(公告)号：US20090062536A1

    公开(公告)日：2009-03-05

    申请号：US12193236

    申请日：2008-08-18

    申请人： KOICHI ARAKI ,  Yoshitaka Sato ,  Shinichi Shirakura ,  Keiji Endo ,  Shin Nakamura ,  Seiji Ukawa ,  Chieko Ueno

    发明人： KOICHI ARAKI ,  Yoshitaka Sato ,  Shinichi Shirakura ,  Keiji Endo ,  Shin Nakamura ,  Seiji Ukawa ,  Chieko Ueno

    IPC分类号： C07D251/30 ,  C07D239/48

    CPC分类号： C07D239/52 ,  A01N43/54 ,  A01N43/66 ,  C07D239/34 ,  C07D251/26

    摘要： A herbicide comprising as an active ingredient a sulfonanilide compound represented by the formula: wherein, R1 represents CHF2 or CH2CF3; R2 represents hydrogen, C1-3 alkyl, 3-propenyl, or 3-propynyl; R3 represents hydrogen; R4 represents hydrogen, hydroxy, or methylthio, or R3 and R4, together with a carbon atom to which they are bonded, may form C═O; R5 represents halogen or methyl; X represents methoxy or chlorine; and Z represents CH or N; with the proviso that, (i) when R1 represents CH2CF3, R2 represents hydrogen, R5 represents bromine or iodine, X represents methoxy, and Z represents CH, (ii) when R1 represents CHF2 and X represents methoxy, R5 represents bromine or iodine, Z represents N, and R2 represents C1-3 alkyl, 3-propenyl, or 3-propynyl, (iii) when R1 represents CHF2 and X represents chlorine, Z represents CH.

    摘要翻译： 一种除草剂，其包含由下式表示的磺酰苯胺化合物作为活性成分：其中，R 1表示CHF 2或CH 2 CF 3; R 2表示氢，C 1-3烷基，3-丙烯基或3-丙炔基; R3表示氢; R4表示氢，羟基或甲硫基，或R3和R4与它们所键合的碳原子一起形成C-O; R5代表卤素或甲基; X表示甲氧基或氯; Z表示CH或N; 条件是（i）当R 1表示CH 2 CF 3时，R 2表示氢，R 5表示溴或碘，X表示甲氧基，Z表示CH，（ii）当R 1表示CHF 2且X表示甲氧基时，R 5表示溴或碘， Z表示N，R2表示C1-3烷基，3-丙烯基或3-丙炔基，（iii）当R1表示CHF2且X表示氯时，Z表示CH。

    Modified Promoter
    28.
    发明申请
    Modified Promoter 失效
    修饰的启动子

    公开(公告)号：US20080014608A1

    公开(公告)日：2008-01-17

    申请号：US10589960

    申请日：2005-03-04

    申请人： Keiji Endo ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Katsuya Ozaki

    IPC分类号： C12P21/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11

    CPC分类号： C12N15/75 ,  C07K14/32 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/2417 ,  C12N9/2437

    摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.

    摘要翻译： 本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。 由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

    Mutant alpha-amylases
    29.
    发明申请
    Mutant alpha-amylases 审中-公开
    突变型α-淀粉酶

    公开(公告)号：US20050181493A1

    公开(公告)日：2005-08-18

    申请号：US10988529

    申请日：2004-11-16

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/28 ,  C12N15/56 ,  C11D7/42

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。