公开(公告)号：US06214612B1

公开(公告)日：2001-04-10

申请号：US08806801

申请日：1997-03-03

申请人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

发明人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

IPC分类号： C12M134

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： A cholesterol sensor for quantitative determination of cholesterol is provided containing an electrode system and a reaction reagent system. The electrode system contains a measuring electrode such as a carbon electrode and a counter electrode, and the reaction reagent system contains cholesterol dehydrogenase, nicotinamide adenine dinucleotide and an oxidized electron mediator. Electron mediators include ferricyanide, 1,2-naphthoquinone-4-sulfonate, 2,6-dichlorophenol indophenol, dimethylbenzoquinone, 1-methoxy-5-methylphenazinium sulfate, methylene blue, gallocyanine, thionine, phenazine methosulfate and Meldola's blue. Diaphorase, cholesterol esterase and a surfactant may also be present. The electrode system is on an insulating base plate, and the base plate has a covering member containing a groove that is a sample supplying channel which extends from an end of the base plate to the electrode system. A reaction layer containing the reagent system in dry form and a layer of a hydrophilic polymer is provided on the base plate or the covering member, or on both the electrode system and covering member so as to be exposed to the sample supplying channel. During operation, the electron mediator is reduced in conjunction with oxidation of cholesterol in a sample by cholesterol dehydrogenase, and an amount of current required to electrochemically re-oxidize the electron mediator is directly proportional to a quantity of cholesterol present in the sample.

摘要翻译： 提供了用于定量测定胆固醇的胆固醇传感器，其包含电极系统和反应试剂系统。 电极系统含有碳电极和对电极等测定电极，反应试剂系统含有胆固醇脱氢酶，烟酰胺腺嘌呤二核苷酸和氧化电子介体。 电子介体包括铁氰化物，1,2-萘醌-4-磺酸盐，2,6-二氯苯酚靛酚，二甲基苯并醌，1-甲氧基-5-甲基吩嗪鎓硫酸盐，亚甲基蓝，花青素，硫堇，吩嗪甲硫酸盐和梅尔多拉蓝。 也可以存在心律变性酶，胆固醇酯酶和表面活性剂。 电极系统在绝缘基板上，基板具有包含作为从基板的端部延伸到电极系统的样品供给路径的槽的覆盖部件。 含有干式试剂体系和亲水性聚合物层的反应层设置在基板或覆盖部件上，或电极系统和覆盖部件上，以暴露于样品供应通道。 在操作期间，电子介体与胆固醇脱氢酶在样品中的胆固醇氧化一起被还原，并且电化学再氧化电子介体所需的电流量与样品中存在的胆固醇量成正比。

公开(公告)号：US5651869A

公开(公告)日：1997-07-29

申请号：US605391

申请日：1996-02-22

申请人： Toshihiko Yoshioka ,  Shin Ikeda ,  Shiro Nankai

发明人： Toshihiko Yoshioka ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N27/26 ,  C12Q1/64

CPC分类号： C12Q1/006 ,  C12Q1/004 ,  Y10S435/817

摘要： The biosensor of this invention includes an electrically insulating substrate; an electrode system formed on the substrate which includes a working electrode and a counter electrode; and a reaction layer formed on the substrate or above the substrate with a space therebetween. The reaction layer includes a pyranose-oxidizing enzyme.

摘要翻译： 本发明的生物传感器包括电绝缘基板; 形成在所述基板上的包括工作电极和对电极的电极系统; 以及形成在基板上或基板上方的反应层，其间具有空间。 反应层包括吡喃糖氧化酶。

公开(公告)号：US06790327B2

公开(公告)日：2004-09-14

申请号：US10023734

申请日：2001-12-21

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27327

CPC分类号： C12Q1/004

摘要： An apparatus for determining the concentration of a substrate in a sample solution using an electrode system comprising a working electrode, a counter electrode, and a reaction layer which contains at least an oxidoreductase and an electron mediator and is formed on the electrode system to electrochemically measure a reduced amount of the electron mediator resulting from enzyme reaction in the reaction layer, wherein a third electrode is formed as an interfering substance detecting electrode. A current flowing between the counter electrode and the third electrode is measured which is taken as a positive error. Subsequently, voltage application between the counter electrode and the third electrode is released and a voltage for oxidizing the reduced form electron mediator is applied between the working electrode and the counter electrode to measure a current flowing between the two electrodes.

摘要翻译： 一种用于使用包括工作电极，对电极和至少包含氧化还原酶和电子介体的反应层的电极系统来确定样品溶液中基底的浓度的装置，并且形成在电极系统上以电化学测量 在反应层中由酶反应产生的电子介体的量减少，其中形成第三电极作为干扰物质检测电极。 测量在对电极和第三电极之间流动的电流，其被认为是正误差。 随后，释放对电极和第三电极之间的电压施加，并且在工作电极和对电极之间施加用于氧化还原形式的电子介体的电压，以测量在两个电极之间流动的电流。

公开(公告)号：US5985130A

公开(公告)日：1999-11-16

申请号：US834829

申请日：1997-04-03

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/416 ,  G01N27/49 ,  G01N27/26

CPC分类号： C12Q1/001 ,  G01N27/3273

摘要： The present invention provides a method for quantifying a substrate having improved measurement accuracy and measurement sensitivity. According to the method for quantifying the substrate of the present invention, a constant voltage is applied to an electrode system, between a working electrode and a counter electrode, via a resistor connected in series with the electrode system after placing a sample solution containing the substrate on the electrode system, so as to oxidize an electron acceptor, which has been reduced by an enzyme reaction of the substrate, thereby determining a concentration of the substrate included in the sample solution based on an oxidizing current flowing in the course of oxidation.

摘要翻译： 本发明提供了一种用于量化具有改进的测量精度和测量灵敏度的衬底的方法。 根据本发明的基板的定量化方法，在放置含有基板的样品溶液之后，通过与电极系统串联连接的电阻器将恒定电压施加到工作电极与对电极之间的电极系统 在电极系统上，以通过基板的酶反应而还原的电子受体氧化，从而基于在氧化过程中流动的氧化电流来确定样品溶液中包含的底物的浓度。

公开(公告)号：US5650062A

公开(公告)日：1997-07-22

申请号：US526557

申请日：1995-09-12

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Syoji Miyazaki

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Syoji Miyazaki

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.

摘要翻译： 本发明的生物传感器可以通过电化学测量由底物和氧化还原酶之间的反应中产生的电子而减少的电子受体的量来量化样品液体中的底物。 生物传感器具有电绝缘基板和形成在基板上的电极系统，该基板包括工作电极，对电极和用于检测液体接合点的第三电极。 第三电极可以仅用于检测液体接点，也可以用作参考电极和液体接点检测电极。

公开(公告)号：US06258254B1

公开(公告)日：2001-07-10

申请号：US09122628

申请日：1998-07-27

申请人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01F164

CPC分类号： C12Q1/004

摘要： A method using a biosensor is disclosed which facilitates high accuracy quantitation of a substrate by best eliminating adverse effects of red blood cell existing in blood together with the substrate. The biosensor comprises an electrode system including a working electrode and a counter electrode formed on an electrically insulating base plate, and a reaction layer containing at least an oxidoreductase and an electron acceptor, wherein the electron acceptor is a sodium salt.

摘要翻译： 公开了一种使用生物传感器的方法，其通过最好地消除血液中存在于血液中的红细胞与基质一起的不良影响，促进了基质的高精度定量。 生物传感器包括电极系统，其包括在电绝缘基板上形成的工作电极和对电极，以及至少包含氧化还原酶和电子受体的反应层，其中电子受体是钠盐。

公开(公告)号：US5985116A

公开(公告)日：1999-11-16

申请号：US989426

申请日：1997-12-12

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12M1/00 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001

摘要： The biosensor of the present invention has a reaction layer that contains at least an oxidoreductase and an electron acceptor and is formed on an electrode system including a working electrode and a counter electrode on a base plate. The working electrode, the counter electrode, and lead conductors connecting with these electrodes are made of carbon. The counter electrode is formed in a substantially C shape, and the working electrode is arranged in a recess of the substantially C-shaped counter electrode. An insulating layer surrounds the counter electrode and the working electrode. A cover member with a groove is combined with the base plate to define a sample supply channel that is formed therebetween and runs toward the electrode system. The groove extends from one end of the base plate and passes over the electrode system, and the insulating layer extends to an air hole formed in a terminal of the groove of the cover member. This arrangement prevents exposure of part of the lead conductor connecting with the working electrode to the sample solution and development of an error in measurement.

摘要翻译： 本发明的生物传感器具有至少包含氧化还原酶和电子受体的反应层，并且形成在基板上的包括工作电极和对电极的电极系统上。 与这些电极连接的工作电极，对电极和引线导体由碳制成。 对电极形成为大致C形，工作电极配置在基本上为C形的对电极的凹部中。 绝缘层包围对电极和工作电极。 具有凹槽的盖构件与基板结合以限定形成在其间并朝向电极系统延伸的样品供应通道。 凹槽从基板的一端延伸并且经过电极系统，并且绝缘层延伸到形成在盖构件的凹槽的端子中的空气孔。 这样可以防止与工作电极连接的引出导体的一部分暴露于样品溶液，并且产生测量误差。

公开(公告)号：US5575895A

公开(公告)日：1996-11-19

申请号：US452774

申请日：1995-05-30

申请人： Shin Ikeda ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor for quantification of a specific component contained in various biological samples comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode which are provided on the electrically insulating base plate, a reaction layer formed on the electrode system including at least an oxido-reductase, and an enclosure member having a hollow space constituting a sample supplying channel on the electrically insulating base plate, wherein substantially the whole of the reaction layer is exposed to the hollow space.

摘要翻译： 用于定量各种生物样品中包含的特定成分的生物传感器包括电绝缘基板，包括设置在电绝缘基板上的工作电极和对电极的电极系统，形成在电极系统上的反应层，包括 至少一种氧化还原酶，以及具有中空空间的封闭构件，该中空空间构成电绝缘基板上的样品供应通道，其中基本上整个反应层暴露于中空空间。

公开(公告)号：US06349229B1

公开(公告)日：2002-02-19

申请号：US09445557

申请日：2000-03-07

申请人： Motokazu Watanabe ,  Shin Ikeda ,  Toshihiko Yoshioka

发明人： Motokazu Watanabe ,  Shin Ikeda ,  Toshihiko Yoshioka

IPC分类号： A61B505

CPC分类号： A61B5/15142 ,  A61B5/150022 ,  A61B5/150099 ,  A61B5/150229 ,  A61B5/150358 ,  A61B5/150412 ,  A61B5/150954 ,  A61B5/15113 ,  A61B5/15117 ,  A61B5/15136

摘要： An examination system for bodily fluids includes a vessel-like examination unit having an opening, a sensor for examining the bodily fluids, and a bodily-fluids-oozing device. This system is further provided with a pump for decompressing the inside of the unit and an electromagnetic cross-valve for releasing the decompressed condition, or still further provided with humidifying means for supplying vapor inside the unit, in addition to the pump and the valve. As a result, the system produces a precise measurement with a little amount of bodily fluid.

摘要翻译： 体液检查系统包括具有开口的容器状检查单元，用于检查体液的传感器和体液渗出装置。 该系统还具有用于对单元内部进行减压的泵和用于释放减压状态的电磁交叉阀，或者还设置有除了泵和阀之外还用于在单元内供应蒸气的加湿装置。 结果，该系统产生一些精确的测量与少量的体液。

公开(公告)号：US5958199A

公开(公告)日：1999-09-28

申请号：US815760

申请日：1997-03-12

申请人： Yoshiko Miyamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Yoshiko Miyamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： Disclosed is a high performance biosensor free from the effect of any components existing in a sample solution other than a substrate. The biosensor comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on the base plate, and a reaction layer containing a hydrophilic polymer, an enzyme and an electron acceptor located over the electrode system. The reaction layer contains an excess amount of hydrophilic polymer in a range of 150 to 1,000% by weight of the enzyme.

摘要翻译： 本发明公开了一种高效能的生物传感器，不受基片以外的样品溶液中存在的任何成分的影响。 生物传感器包括电绝缘基板，包括设置在基板上的工作电极和对电极的电极系统，以及包含位于电极系统上的亲水性聚合物，酶和电子受体的反应层。 反应层含有过量的亲水性聚合物，其量为该酶的150-1000重量％。