公开(公告)号：US11542555B2

公开(公告)日：2023-01-03

申请号：US15929784

申请日：2020-05-21

Applicant: TEMPLE UNIVERSITY OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

Inventor： Arthur M. Feldman ,  Douglas G. Tilley ,  Weizhong Zhu ,  Kamel Khalili ,  Walter J. Koch

IPC: C12Q1/6883 ,  G01N33/68 ,  A61K48/00

Abstract: Compositions are directed to BCL2-associated athanogene 3 (BAG3) molecules and agents which modulate expression of BAG3 molecules. Pharmaceutical composition for administration to patients, for example, patients with heart failure, comprise one or more BAG3 molecules or agents which modulate expression of BAG3. Methods of treatment and identifying candidate therapeutic agents are also provided.

公开(公告)号：US11291710B2

公开(公告)日：2022-04-05

申请号：US16874295

申请日：2020-05-14

Applicant: Temple University of The Commonwealth System of Higher Education

Inventor： Kamel Khalili ,  Wenhui Hu

IPC: A61K38/46 ,  C12N15/11 ,  A61K48/00 ,  A61K9/00 ,  A61K35/12 ,  A61K45/06 ,  C12N7/00 ,  C12N9/22

Abstract: A method of preventing transmission of a retrovirus from a mother to her offspring, by administering to the mother a therapeutically effective amount of a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and the two or more different multiplex gRNAs, wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) of proviral DNA of the virus that is unique from the genome of the host cell, cleaving a double strand of the proviral DNA at a first target protospacer sequence with the CRISPR-associated endonuclease, cleaving a double strand of the proviral DNA at a second target protospacer sequence with the CRISPR-associated endonuclease, excising an entire HIV-1 proviral genome, eradicating the HIV-1 proviral DNA from the host cell, and preventing transmission of the proviral DNA to the offspring.

公开(公告)号：US20210128554A1

公开(公告)日：2021-05-06

申请号：US16472462

申请日：2017-12-22

Applicant: Temple University - of the Commonwealth System of Higher Education

Inventor： Kamel Khalili

IPC: A61K31/505 ,  A61P31/14 ,  A61K45/06

Abstract: Compositions of non-nucleoside reverse transcriptase inhibitor (NNRTI) and/or a nucleoside reverse transcriptase inhibitor (NRTI) or combinations thereof, in the prevention and treatment of Flaviviridae infections, e.g. Zika virus.

公开(公告)号：US20180228875A1

公开(公告)日：2018-08-16

申请号：US15950227

申请日：2018-04-11

Applicant: Temple University of the Commonwealth System of Higher Education

Inventor： Kamel Khalili ,  Wenhui HU

IPC: A61K38/46 ,  A61K9/00 ,  C12N9/22 ,  C12N7/00 ,  C12N15/11 ,  A61K45/06 ,  A61K48/00 ,  A61K35/12

CPC classification number: A61K38/465 ,  A61K9/0034 ,  A61K35/12 ,  A61K45/06 ,  A61K48/00 ,  A61K48/005 ,  C12N7/00 ,  C12N9/22 ,  C12N15/111 ,  C12N2310/20 ,  C12N2320/30 ,  C12N2740/16063 ,  C12Y301/21

Abstract: A personalized method of inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus, by determining a nucleic acid sequence of the HIV-1 proviral DNA harbored by a subject, designing two or more different multiplex guide RNAs (gRNAs) complementary to the HIV-1 proviral DNA sequences in the subject, administering to the subject a therapeutically effective amount of a composition comprising a CRISPR-associated endonuclease, and the two or more different multiplex gRNAs, cleaving a double strand of the proviral DNA at a first target protospacer sequence with the CRISPR-associated endonuclease, cleaving a double strand of the proviral DNA at a second target protospacer sequence with the CRISPR-associated endonuclease, excising an entire HIV-1 proviral genome, and eradicating the HIV-1 proviral DNA from the host cell.

公开(公告)号：US20180214521A1

公开(公告)日：2018-08-02

申请号：US15939710

申请日：2018-03-29

Applicant: Temple University of the Commonwealth System of Higher Education

Inventor： Kamel Khalili ,  Wenhui Hu

IPC: A61K38/46 ,  C12N15/11 ,  A61K35/12 ,  A61K48/00 ,  C12N7/00 ,  C12N9/22 ,  A61K9/00 ,  A61K45/06

CPC classification number: A61K38/465 ,  A61K9/0034 ,  A61K35/12 ,  A61K45/06 ,  A61K48/00 ,  A61K48/005 ,  C12N7/00 ,  C12N9/22 ,  C12N15/111 ,  C12N2310/20 ,  C12N2320/30 ,  C12N2740/16063 ,  C12Y301/21

Abstract: A method of immunizing a subject at risk of HIV-1 virus infection, by administering to the subject a prophylactically effective amount of a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and two or more different multiplex guide RNAs (gRNAs), wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) of proviral DNA of the virus that is unique from the genome of the host cell, cleaving a double strand of the proviral DNA at a first target protospacer sequence with the CRISPR-associated endonuclease, cleaving a double strand of the proviral DNA at a second target protospacer sequence with the CRISPR-associated endonuclease, excising an entire HIV-1 proviral genome, eradicating the HIV-1 proviral DNA from the host cell, and preventing HIV-1 virus infection in the subject.

公开(公告)号：US20180073018A1

公开(公告)日：2018-03-15

申请号：US15705825

申请日：2017-09-15

Applicant: Temple University - of the Commonwealth System of Higher Education

Inventor： Wenhui Hu ,  Kamel Khalili ,  Yonggang Zhang

IPC: C12N15/11 ,  A61K48/00 ,  C12N7/00 ,  C12N9/22 ,  C12N15/113 ,  C07K14/005

CPC classification number: C12N15/11 ,  A61K39/21 ,  A61K48/005 ,  C07K14/005 ,  C07K2319/71 ,  C12N7/00 ,  C12N9/22 ,  C12N15/1132 ,  C12N2310/16 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2740/16022 ,  C12N2740/16051 ,  C12N2740/16062 ,  C12N2795/18122 ,  C12N2795/18133 ,  C12N2800/40

Abstract: The present invention relates to compositions and methods for reactivation of HIV in vivo or in vitro. latently infected cell. In one embodiment, the present invention uses the CRISPR/Cas9 system in combination with the MS2 bacteriophage coat protein-mediated synergistic activation mediator (SAM) system to provide enhanced transcriptional activation of the HIV genome.

公开(公告)号：US20150010901A1

公开(公告)日：2015-01-08

申请号：US14375263

申请日：2013-01-29

Applicant: TEMPLE UNIVERSITY - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

Inventor： Kamel Khalili ,  Ilker K. Sariyer

IPC: G01N33/569

CPC classification number: G01N33/56983 ,  G01N2333/025 ,  G01N2469/10 ,  G01N2469/20 ,  G01N2800/50 ,  G01N2800/56

Abstract: The invention provides methods of detection and monitoring of polyomavirus reactivation and active polyomavirus infections using a biological fluid sample. Also provided are methods of risk assessment and risk monitoring of developing a polyomavirus-associated disease.

Abstract translation: 本发明提供使用生物流体样品检测和监测多瘤病毒再活化和活性多瘤病毒感染的方法。 还提供了开发多瘤病毒相关疾病的风险评估和风险监测方法。

公开(公告)号：US20240009221A1

公开(公告)日：2024-01-11

申请号：US18300468

申请日：2023-04-14

Applicant: Temple University - of the Commonwealth System of Higher Education

Inventor： Kamel Khalili ,  Shuren Liao ,  Ilker K. Sariyer

IPC: A61K31/706 ,  A61K31/505 ,  A61P31/14

CPC classification number: A61K31/706 ,  A61K31/505 ,  A61P31/14

Abstract: Compositions for prevention or treatment for the SARS family of coronaviruses, e.g., SARS-CoV-2 and SARS-OC43 (common cold virus), include non-nucleoside reverse transcriptase inhibitors (NNRTI), such as rilpivirine, and nucleoside-analogue antiviral agents, such as remdesivir.

公开(公告)号：US20230287401A1

公开(公告)日：2023-09-14

申请号：US17931018

申请日：2022-09-09

Applicant: Temple University - of the Commonwealth System of Higher Education

Inventor： Kamel Khalili ,  Hassen Wollebo

IPC: C12N15/11 ,  A61P31/20 ,  A61K31/7088 ,  A61K38/46 ,  C12N9/22

CPC classification number: C12N15/11 ,  A61P31/20 ,  A61K31/7088 ,  A61K38/465 ,  C12N9/22 ,  A61K38/46 ,  C12N2310/20 ,  C12N2800/80

Abstract: Compositions that specifically cleave target sequences in Hepadnaviridae, for example Hepatitis B virus (HBV) include nucleic acids encoding a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) associated endonuclease and a guide RNA sequence complementary to a target sequence in HBV. These compositions are administered to a subject for eradicating an infection, latent or otherwise, or at risk for contracting HBV infection.

公开(公告)号：US20230167425A1

公开(公告)日：2023-06-01

申请号：US17837352

申请日：2022-06-10

Applicant: Temple University - of The Commonwealth System of higher Education

Inventor： Kamel Khalili ,  Wollebo Hassen

IPC: C12N9/22 ,  C12N15/113 ,  A61K38/46 ,  C12N15/11

CPC classification number: C12N9/22 ,  C12N15/1133 ,  A61K38/465 ,  C12N15/11 ,  C12Q1/705

Abstract: Compositions that specifically cleave target sequences in Herpesviridae, for example Varicella zoster virus (VZV) include nucleic acids encoding a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) associated endonuclease and a guide RNA sequence complementary to a target sequence in VZV. These compositions are administered to a subject for treating an infection or at risk for contracting a VZV infection.