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21.发明授权
公开(公告)号：US5654180A
公开(公告)日：1997-08-05
申请号：US344683
申请日：1994-11-17
摘要： The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus, said hybrid plasmid vector comprising an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to the genus Rhodococcus, and an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to Escherichia coli, and an isolated DNA sequence containing a drug resistance gene.
摘要翻译： 本发明提供了一种重组质粒，其包含将杂交质粒载体与编码来自属于红球菌属的细菌的腈降解酶的一个或多个基因的分离的DNA序列组合，所述杂交质粒载体包含赋予的分离的DNA序列 载体能够在属于红球菌属的细菌的细胞中复制和扩增的能力，以及赋予载体在分离的DNA序列的细胞中复制和扩增能力的分离的DNA序列 含有耐药性基因。
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22.发明授权Substitutional variants of hydroxynitrile lyase with high specific activity and methods of use 失效具有高比活性和使用方法的羟基腈裂解酶的替代变体公开(公告)号：US08030053B2
公开(公告)日：2011-10-04
申请号：US11665491
申请日：2005-10-14
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译： 改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性 引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。
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公开(公告)号：US20090170156A1
公开(公告)日：2009-07-02
申请号：US11665491
申请日：2005-10-14
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
摘要翻译： 改进的羟基腈裂解酶，其特征在于在野生型羟基腈裂解酶的氨基酸序列中与另一个氨基酸具有至少一个氨基酸残基的取代突变，并且其转化体的羟基腈裂解酶活性高于每个转化体的羟基腈裂解酶活性 引入野生型羟基腈裂解酶基因的转化体; 以及生产羟基腈裂解酶的方法，包括在宿主中表达改进的羟基腈裂解酶，并从所得培养物中回收改良的羟基腈裂解酶。
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公开(公告)号：US20070117119A1
公开(公告)日：2007-05-24
申请号：US11514162
申请日：2006-09-01
申请人： Takashi Akita , Chiho Ito , Teruta Ishimaru , Haruko Miyauchi , Kei Murase , Atsushi Takahashi , Toshinori Sumi , Osamu Maehara , Tadanobu Ikeda , Nobuko Oogami , Takayuki Makino , Fujio Yu , Fumiaki Watanabe , Toshitaka Uragaki , Wataru Fujii , Takeharu Morishita
发明人： Takashi Akita , Chiho Ito , Teruta Ishimaru , Haruko Miyauchi , Kei Murase , Atsushi Takahashi , Toshinori Sumi , Osamu Maehara , Tadanobu Ikeda , Nobuko Oogami , Takayuki Makino , Fujio Yu , Fumiaki Watanabe , Toshitaka Uragaki , Wataru Fujii , Takeharu Morishita
CPC分类号： D06M23/00 , D06M15/13 , D06M15/15 , D06M15/263 , D06M15/285 , D06M15/333 , D06M15/3562 , D06M15/53 , D06M16/00 , D06M23/02 , Y10T156/1052 , Y10T436/143333
摘要： The present invention relates to biological substance-immobilized fibers wherein a biological substance is immobilized on a fiber, fibers retaining a biological substance-immobilized gel, and fiber alignments having bundles of the above-described fibers and slices of the same.
摘要翻译： 本发明涉及生物物质固定化纤维，其中将生物物质固定在纤维上，保留固定有生物物质的凝胶的纤维，以及具有上述纤维束和纤维的纤维排列。
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公开(公告)号：US07122378B1
公开(公告)日：2006-10-17
申请号：US09914782
申请日：2000-03-06
申请人： Takashi Akita , Chiho Ito , Teruta Ishimaru , Haruko Miyauchi , Kei Murase , Atsushi Takahashi , Toshinori Sumi , Osamu Maehara , Tadanobu Ikeda , Nobuko Oogami , Takayuki Makino , Fujio Yu , Fumiaki Watanabe , Toshitaka Uragaki , Wataru Fujii , Takeharu Morishita
发明人： Takashi Akita , Chiho Ito , Teruta Ishimaru , Haruko Miyauchi , Kei Murase , Atsushi Takahashi , Toshinori Sumi , Osamu Maehara , Tadanobu Ikeda , Nobuko Oogami , Takayuki Makino , Fujio Yu , Fumiaki Watanabe , Toshitaka Uragaki , Wataru Fujii , Takeharu Morishita
CPC分类号： D06M23/00 , D06M15/13 , D06M15/15 , D06M15/263 , D06M15/285 , D06M15/333 , D06M15/3562 , D06M15/53 , D06M16/00 , D06M23/02 , Y10T156/1052 , Y10T436/143333
摘要： By the present invention, there is provided a fiber having nucleic acid immobilized thereon, an alignment of fibers having nucleic acid immobilized thereon, and a slice thereof.
摘要翻译： 通过本发明，提供了其上固定有核酸的纤维，其上固定有核酸的纤维的取向及其切片。
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公开(公告)号：US5246857A
公开(公告)日：1993-09-21
申请号：US773055
申请日：1991-10-08
申请人： Fujio Yu , Yoshihiro Hashimoto
发明人： Fujio Yu , Yoshihiro Hashimoto
IPC分类号： C12N15/74
CPC分类号： C12N15/74
摘要： The present invention provides an isolated circular plasmid of about 2.6 kb derived from a bacterium belonging to the genus Rhodococcus whose restriction sites compises two SacI sites, one BamII site, one PvuI site, one ScaI site, one SphI site and one XhoI site. In addition, the present invention provides an isolated circular plasmid of about 7.6 kb derived from a bacterium belonging to the genus Rhodococcus whose restriction sites compises one SphI site, two KpnI sites, one BglII site, and three SacI sites. The vectors of the invention can be suitable for Rhodococcus hosts and usefull in industry.
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