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    Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells
    22.
    发明申请
    Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells 审中-公开
    具有固定的可降解阳离子聚合物的固体表面用于转染真核细胞

    公开(公告)号：US20060134790A1

    公开(公告)日：2006-06-22

    申请号：US11303082

    申请日：2005-12-14

    申请人： Yasunobu Tanaka ,  Chris Castello ,  Lei Yu

    发明人： Yasunobu Tanaka ,  Chris Castello ,  Lei Yu

    IPC分类号： C12N15/87 ,  C12N15/82 ,  C12M1/42

    CPC分类号： C12N5/0068 ,  C12M23/20 ,  C12M35/00 ,  C12N2510/00 ,  C12N2533/30

    摘要： A cell transfection/culture device is disclosed which includes a solid support coated with a degradable polymer cation as a transfection reagent. The transfection/culture device is conveniently stored at room temperature until use. Cell transfection is accomplished easily by adding the nucleic acid of interest and the cells to be transfected to the transfection/culture device. Cell transfection is completed in less than one hour by using the transfection/culture device described herein.

    摘要翻译： 公开了一种细胞转染/培养装置，其包括涂覆有可降解聚合物阳离子作为转染试剂的固体支持物。 转染/培养装置在室温下方便储存直至使用。 通过将感兴趣的核酸和待转染的细胞加入到转染/培养装置中来容易地完成细胞转染。 通过使用本文所述的转染/培养装置在小于1小时内完成细胞转染。

    Intracellular peptide delivery
    23.
    发明申请
    Intracellular peptide delivery 有权
    细胞内肽递送

    公开(公告)号：US20060083737A1

    公开(公告)日：2006-04-20

    申请号：US11251956

    申请日：2005-10-17

    申请人： Kenjiro Minomi ,  Lei Yu

    发明人： Kenjiro Minomi ,  Lei Yu

    IPC分类号： A61K39/395 ,  A61K39/21 ,  A61K38/43

    CPC分类号： A61K38/47 ,  A61K47/183 ,  A61K47/59 ,  A61K47/645 ,  A61K47/6901 ,  C07K14/005 ,  C07K2319/10 ,  C12N15/87 ,  C12N2740/16322

    摘要： Methods and kits for transporting heterologous compounds into cells, particularly mammalian cells, are disclosed. The method involves the pretreatment of the cell with a polycation, cationic polymer and/or cationic peptide before transportation of the heterologous compound into the cell. The methods are particularly applicable to the transport of peptides and proteins.

    摘要翻译： 公开了将异源化合物转运到细胞，特别是哺乳动物细胞中的方法和试剂盒。 该方法包括在将异源化合物运送到细胞内之前用聚阳离子阳离子聚合物和/或阳离子肽预处理细胞。 该方法特别适用于肽和蛋白质的转运。

    Neural systems with range reducers and/or extenders
    26.
    发明授权
    Neural systems with range reducers and/or extenders 失效
    带范围减速器和/或增量器的神经系统

    公开(公告)号：US06601051B1

    公开(公告)日：2003-07-29

    申请号：US08893782

    申请日：1997-07-11

    申请人： James Ting-Ho Lo ,  Lei Yu

    发明人： James Ting-Ho Lo ,  Lei Yu

    IPC分类号： G06F1518

    CPC分类号： H03H21/0012 ,  G06N3/0445 ,  G06N3/084 ,  H03H2222/04

    摘要： A neural system is disclosed for processing an exogenous input process to produce a good outward output process with respect to a performance criterion, even if the range of one or both of these processes is necessarily large and/or keeps necessarily expanding during the operation of the neural system. The disclosed neural system comprises a recurrent neural network (RNN) and at least one range extender or reducer, each of which is a dynamic transformer. A range reducer transforms dynamically at least one component of the exogenous input process into inputs to at least one input neuron of said RNN. A range extender transforms dynamically outputs of at least one output neuron of said RNN into at least one component of the outward output process. There are many types of range extender and reducer, which have different degrees of effectiveness and computational costs. For a neural system under design, the types of range extenders and/or reducers are selected jointly with the architecture of the RNN in consideration of the neural system's processing performance with respect to the mentioned performance criterion, the RNN's size and the computational cost of selected range extenders and reducers so as to maximize the cost effectiveness of the neural system.

    摘要翻译： 公开了一种用于处理外部输入过程以产生相对于性能标准的良好的向外输出过程的神经系统，即使这些过程中的一个或两个的范围必然很大和/或在操作期间必然扩大 神经系统。 所公开的神经系统包括循环神经网络（RNN）和至少一个范围扩展器或减速器，每个延伸器或减速器都是动态变压器。 范围缩减器将动态地将外部输入过程的至少一个分量变换成对所述RNN的至少一个输入神经元的输入。 范围扩展器将所述RNN的至少一个输出神经元的动态输出转换成向外输出过程的至少一个分量。 范围扩展器和减速器有许多种类，具有不同程度的有效性和计算成本。 对于设计中的神经系统，考虑到关于上述性能标准的神经系统的处理性能，RNN的尺寸和所选择的计算成本，扩展器和/或减速器的类型与RNN的架构联合选择 扩展器和减速器，以最大化神经系统的成本效益。

    Alleles of the human mu opioid receptor and diagnostic methods based thereon
    27.
    发明授权
    Alleles of the human mu opioid receptor and diagnostic methods based thereon 失效
    人类μ阿片受体的等位基因及其诊断方法

    公开(公告)号：US06335168B1

    公开(公告)日：2002-01-01

    申请号：US09351198

    申请日：1999-07-09

    申请人： Mary Jeanne Kreek ,  Karl Steven LaForge ,  Lei Yu ,  Jay A. Tischfield

    发明人： Mary Jeanne Kreek ,  Karl Steven LaForge ,  Lei Yu ,  Jay A. Tischfield

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6883 ,  C12Q2600/156

    摘要： Provided herein are variant alleles of a gene encoding a mu opioid receptor, along with cloning vectors for replicating such variant alleles, expressing vectors for expressing the variant alleles to produce variant mu opioid receptors, and antibodies to such variant receptors. Also disclosed are binding characteristics of such variant receptors regarding binding to opioid ligands, and the using of such binding characteristics to diagnose a subjects susceptibility to pain, susceptibility to an addictive disease, selecting an appropriate pain reliever along with a therapeutically effective amount of the reliever to administer to a subject suffering from pain. In addition, diagnostic methods for diagnosing a disease or disorder such as infertility, constipation, diarrhea, decreased immune response relative to a standard, and decreased ability to withstand stress relative to a standard, along with commercial kits for diagnosing such diseases or disorders. Furthermore, the invention is also directed to identification of targeted prevention methods, early therapeutic intervention, and improved treatment of opioid addiction, infertility, constipation, diarrhea, impaired immune responsiveness, and stress.

    摘要翻译： 本文提供了编码μ阿片受体的基因的变异等位基因，以及用于复制这种变异等位基因的克隆载体，表达用于表达变体等位基因以产生变体μ阿片样物质受体的载体，以及针对这种变体受体的抗体。 还公开了这样的变体受体关于结合阿片样物质配体的结合特征，以及使用这种结合特征来诊断受试者对疼痛的敏感性，对成瘾性疾病的易感性，选择适当的止痛药以及治疗有效量的缓解剂 给予痛苦的受试者。 此外，用于诊断疾病或病症的诊断方法，例如不育症，便秘，腹泻，相对于标准物的免疫应答降低，以及相对于标准物承受应力的能力降低，以及用于诊断这种疾病或病症的商业试剂盒。 此外，本发明还涉及确定靶向预防方法，早期治疗干预和改善阿片样物质成瘾，不育症，便秘，腹泻，免疫应答受损和应激的治疗。

    Water flosser
    28.
    外观设计
    Water flosser 有权

    公开(公告)号：USD959649S1

    公开(公告)日：2022-08-02

    申请号：US29754732

    申请日：2020-10-14

    申请人： Lei Yu

    设计人： Lei Yu

    Pump body of oral irrigator
    29.
    发明申请
    Pump body of oral irrigator 有权

    公开(公告)号：US20210059800A1

    公开(公告)日：2021-03-04

    申请号：US17095776

    申请日：2020-11-12

    申请人： Lei Yu

    发明人： Lei Yu

    IPC分类号： A61C17/02

    摘要： A pump body of an oral irrigator includes a mount, a motor disposed at a rear end away from the mount, a pinion disposed on a shaft of the motor, an annular gear disposed on a shaft member to mesh with the pinion, a cam disposed in the annular gear and adjacent to one side, a linkage secured to the cam, front and rear plungers disposed on a front end of the linkage, and a cylindrical tube disposed on the plungers in the mount. The rotation of the cam only allows the linkage to repetitively moves in a back-and-forth linear motion. Wear between the plungers and the cylindrical tube is decreased to a minimum and leakage is prevented.