公开(公告)号：US08357499B2

公开(公告)日：2013-01-22

申请号：US11326441

申请日：2006-01-06

申请人： Mark Zoller ,  Hong Xu ,  Lena Staszewski ,  Bryan Moyer ,  Alexey Pronin ,  Jon Elliot Adler ,  Guy Servant ,  Nicholas Callamaras

发明人： Mark Zoller ,  Hong Xu ,  Lena Staszewski ,  Bryan Moyer ,  Alexey Pronin ,  Jon Elliot Adler ,  Guy Servant ,  Nicholas Callamaras

IPC分类号： G01N33/567 ,  C07K14/705 ,  C12N15/12

CPC分类号： C07K14/705

摘要： The present invention relates to isolated nucleic acid sequences that encode human olfactory cyclic nucleotide gated (CNG) channel subunits, and the corresponding polypeptides. The invention further relates to the use of human CNG channels to profile, screen for, and identify compounds that modulate the human olfactory CNG channel. More specifically, the invention relates to the expression of the human olfactory CNG channel in cells, preferably mammalian cells, and the use of these cells in high throughput cell-based assays to identify compounds that enhance or block human olfactory CNG function. Compounds that activate the olfactory CNG channel will enhance smell and can be used to make foods more palatable for individuals with attenuated olfactory function. Conversely, compounds that inhibit the olfactory CNG channel will inhibit smell and can be use to block malodors. Additionally, the invention relates to the use of cell-based olfactory CNG channel assays to identify modulates of G-protein coupled receptor (GPCRs) and other proteins that regulate cyclic nucleotide levels.

公开(公告)号：US07052857B2

公开(公告)日：2006-05-30

申请号：US10189507

申请日：2002-07-08

申请人： Mark Zoller ,  Hong Xu ,  Lena Staszewski ,  Bryan Moyer ,  Alexey Pronin ,  Jon Elliot Adler ,  Guy Servant ,  Nicholas Callamaras

发明人： Mark Zoller ,  Hong Xu ,  Lena Staszewski ,  Bryan Moyer ,  Alexey Pronin ,  Jon Elliot Adler ,  Guy Servant ,  Nicholas Callamaras

IPC分类号： G01N33/567

CPC分类号： C07K14/705

摘要： The present invention relates to isolated nucleic acid sequences that encode human olfactory cyclic nucleotide gated (CNG) channel subunits, and the corresponding polypeptides. The invention further relates to the use of human CNG channels to profile, screen for, and identify compounds that modulate the human olfactory CNG channel. More specifically, the invention relates to the expression of the human olfactory CNG channel in cells, preferably mammalian cells, and the use of these cells in high throughput cell-based assays to identify compounds that enhance or block human olfactory CNG function. Compounds that activate the olfactory CNG channel will enhance smell and can be used to make foods more palatable for individuals with attenuated olfactory function. Conversely, compounds that inhibit the olfactory CNG channel will inhibit smell and can be use to block malodors. Additionally, the invention relates to the use of cell-based olfactory CNG channel assays to identify modulates of G-protein coupled receptor (GPCRs) and other proteins that regulate cyclic nucleotide levels.

摘要翻译： 本发明涉及编码人嗅环核苷酸门控（CNG）通道亚基的分离的核酸序列，以及相应的多肽。 本发明还涉及使用人类CNG通道来分析，筛选和鉴定调节人嗅觉CNG通道的化合物。 更具体地，本发明涉及人嗅觉CNG通道在细胞，优选哺乳动物细胞中的表达，以及这些细胞在高通量细胞分析中的应用，以鉴定增强或阻断人嗅觉CNG功能的化合物。 激活嗅觉CNG通道的化合物将增强气味，可用于使食物对于具有减弱的嗅觉功能的个体更可口。 相反，抑制嗅觉CNG通道的化合物将抑制气味，并可用于阻止恶臭。 另外，本发明涉及使用基于细胞的嗅觉CNG通道测定来鉴定调节环核苷酸水平的G蛋白偶联受体（GPCR）和其他蛋白质的调节。

公开(公告)号：US20110053184A1

公开(公告)日：2011-03-03

申请号：US12901011

申请日：2010-10-08

申请人： Xiaodong Li ,  Alexey Pronin ,  Hong Xu

发明人： Xiaodong Li ,  Alexey Pronin ,  Hong Xu

IPC分类号： G01N33/53

CPC分类号： C07K14/705

摘要： The present invention relates to the discovery of a novel haplotype of the human taste receptor hT2R50 in the T2R taste receptor family that responds to particular bitter ligands, i.e., 2-acetylpyrazine and ethylpyrazine. The present invention also relates to the use of this novel haplotype in assays for identifying ligands that modulate the activation of the hT250 taste receptor. These compounds potentially may be used as additives in foods, beverages and medicinals for modifying (blocking) hT2R50-associated bitter taste.

摘要翻译： 本发明涉及在特异性苦味配体即2-乙酰吡嗪和乙基吡嗪中对T2R味觉受体家族中的人味受体hT2R50的新单体型的发现。 本发明还涉及这种新型单倍型在用于鉴定调节hT250味觉受体的活化的配体的测定中的用途。 这些化合物潜在地可以用作食品，饮料和药物中用于改变（阻断）hT2R50相关苦味的添加剂。

公开(公告)号：US07811788B2

公开(公告)日：2010-10-12

申请号：US11850337

申请日：2007-09-05

申请人： Xiaodong Li ,  Alexey Pronin ,  Hong Xu

发明人： Xiaodong Li ,  Alexey Pronin ,  Hong Xu

IPC分类号： C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N15/74 ,  C07H21/04

CPC分类号： C07K14/705

摘要： The present invention relates to the discovery of a novel haplotype of the human taste receptor hT2R50 in the T2R taste receptor family that responds to particular bitter ligands, i.e., 2-acetylpyrazine and ethylpyrazine. The present invention also relates to the use of this novel haplotype in assays for identifying ligands that modulate the activation of the hT2R50 taste receptor. These compounds potentially may be used as additives in foods, beverages and medicinals for modifying (blocking) hT2R50-associated bitter taste.

摘要翻译： 本发明涉及在特异性苦味配体即2-乙酰吡嗪和乙基吡嗪中对T2R味觉受体家族中的人味受体hT2R50的新单体型的发现。 本发明还涉及这种新型单体型用于鉴定调节hT2R50味觉受体的活化的配体的测定中的用途。 这些化合物潜在地可以用作食品，饮料和药物中用于改变（阻断）hT2R50相关苦味的添加剂。

公开(公告)号：US08221987B2

公开(公告)日：2012-07-17

申请号：US12901011

申请日：2010-10-08

申请人： Xiaodong Li ,  Alexey Pronin ,  Hong Xu

发明人： Xiaodong Li ,  Alexey Pronin ,  Hong Xu

IPC分类号： G01N33/53 ,  G01N33/567 ,  G01N33/569 ,  G01N33/554 ,  G01N33/566 ,  C12Q1/02

CPC分类号： C07K14/705

摘要： The present invention relates to the discovery of a novel haplotype of the human taste receptor hT2R50 in the T2R taste receptor family that responds to particular bitter ligands, i.e., 2-acetylpyrazine and ethylpyrazine. The present invention also relates to the use of this novel haplotype in assays for identifying ligands that modulate the activation of the hT250 taste receptor. These compounds potentially may be used as additives in foods, beverages and medicinals for modifying (blocking) hT2R50-associated bitter taste.

摘要翻译： 本发明涉及在特异性苦味配体即2-乙酰吡嗪和乙基吡嗪中对T2R味觉受体家族中的人味受体hT2R50的新单体型的发现。 本发明还涉及这种新型单倍型在用于鉴定调节hT250味觉受体的活化的配体的测定中的用途。 这些化合物潜在地可以用作食品，饮料和药物中用于改变（阻断）hT2R50相关苦味的添加剂。

公开(公告)号：US08338115B2

公开(公告)日：2012-12-25

申请号：US12413102

申请日：2009-03-27

申请人： Jon Elliot Adler ,  Alexey Pronin ,  Huixian Tang

发明人： Jon Elliot Adler ,  Alexey Pronin ,  Huixian Tang

IPC分类号： G01N33/567 ,  C07K14/705

CPC分类号： C07K14/705 ,  A61K38/00

摘要： Isolated nucleic acids encoding T2R76 polypeptides, recombinantly expressed T2R76 polypeptides, heterologous expression systems for recombinant expression of T2R76 polypeptides, assay methods employing the same, and methods for altering taste perception via administration of a T2R76 modulator. These T22R76 polypeptides can be expressed alone or co-expressed with another T2R polypeptide, preferably a different human T2R polypeptide.

摘要翻译： 编码T2R76多肽的分离的核酸，重组表达的T2R76多肽，用于重组表达T2R76多肽的异源表达系统，使用该多肽的测定方法以及通过施用T2R76调节剂改变味觉的方法。 这些T22R76多肽可以单独表达或与另一个T2R多肽，优选不同的人T2R多肽共表达。

公开(公告)号：US20090203027A1

公开(公告)日：2009-08-13

申请号：US12413102

申请日：2009-03-27

申请人： Jon Elliot Adler ,  Alexey Pronin ,  Huixian Tang ,  Mark Zoller

发明人： Jon Elliot Adler ,  Alexey Pronin ,  Huixian Tang ,  Mark Zoller

IPC分类号： C12Q1/68 ,  G01N33/567 ,  G01N33/569

CPC分类号： C07K14/705 ,  A61K38/00

摘要： Isolated nucleic acids encoding T2R76 polypeptides, recombinantly expressed T2R76 polypeptides, heterologous expression systems for recombinant expression of T2R76 polypeptides, assay methods employing the same, and methods for altering taste perception via administration of a T2R76 modulator. These T22R76 polypeptides can be expressed alone or co-expressed with another T2R polypeptide, preferably a different human T2R polypeptide.

摘要翻译： 编码T2R76多肽的分离的核酸，重组表达的T2R76多肽，用于重组表达T2R76多肽的异源表达系统，使用该多肽的测定方法以及通过施用T2R76调节剂改变味觉的方法。 这些T22R76多肽可以单独表达或与另一个T2R多肽，优选不同的人T2R多肽共表达。

公开(公告)号：US07517972B2

公开(公告)日：2009-04-14

申请号：US10628464

申请日：2003-07-29

申请人： Jon Elliot Adler ,  Huixian Tang ,  Alexey Pronin

发明人： Jon Elliot Adler ,  Huixian Tang ,  Alexey Pronin

IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N1/19 ,  C12N1/21 ,  C12N5/16 ,  C12N5/22

CPC分类号： C07K14/705 ,  A61K38/00

摘要： Isolated nucleic acids encoding T2R76 polypeptides, recombinantly expressed T2R76 polypeptides, heterologous expression systems for recombinant expression of T2R76 polypeptides, assay methods employing the same, and methods for altering taste perception via administration of a T2R76 modulator. These T22R76 polypeptides can be expressed alone or co-expressed with another T2R polypeptide, preferably a different human T2R polypeptide.

摘要翻译： 编码T2R76多肽的分离的核酸，重组表达的T2R76多肽，用于重组表达T2R76多肽的异源表达系统，使用该多肽的测定方法以及通过施用T2R76调节剂改变味觉的方法。 这些T22R76多肽可以单独表达或与另一个T2R多肽，优选不同的人T2R多肽共表达。