公开(公告)号：US06423845B1

公开(公告)日：2002-07-23

申请号：US09718139

申请日：2000-11-21

申请人： Nelo R. Rivera ,  Yi Xiao ,  Nobuyoshi Yasuda

发明人： Nelo R. Rivera ,  Yi Xiao ,  Nobuyoshi Yasuda

IPC分类号： C07D47104

CPC分类号： C07D471/04 ,  C07F9/4006

摘要： A novel process is provided for the preparation of 3-(5,6,7,8-tetrahydro-[1,8]-naphthyridin-2-yl)-propylamine which is useful in the synthesis of &agr;v integrin receptor antagonists. Also provided are useful intermediates obtained from the process.

摘要翻译： 提供了一种制备3-（5,6,7,8-四氢 - [1,8] - 萘啶-2-基） - 丙胺的新方法，其可用于合成αphav整联蛋白受体拮抗剂。 还提供了从该方法获得的有用的中间体。

公开(公告)号：US10725058B2

公开(公告)日：2020-07-28

申请号：US16179336

申请日：2018-11-02

申请人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

发明人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

IPC分类号： G01N33/94 ,  G01N33/52

摘要： The subject invention provides methods, assays, and products for visual detection of small-molecule targets in a sample in both clinical and field settings within minutes. The subject invention is based on an aptamer sensor that reports the presence of small-molecule target via a sensitive colorimetric signal for naked-eye detection. The aptamer sensor is a CBSAzyme-based sensor having both target-mediated cooperative behavior of the CBSA and peroxidase-mimicking catalytic activity of DNAzyme. The subject invention also provides methods of using the CBSAzyme-based sensor.

公开(公告)号：US20160329121A1

公开(公告)日：2016-11-10

申请号：US15146440

申请日：2016-05-04

申请人： Yi Xiao ,  Bhargav Guntupalli ,  Janak Paudyal

发明人： Yi Xiao ,  Bhargav Guntupalli ,  Janak Paudyal

IPC分类号： H01B1/04 ,  H01B1/02

CPC分类号： H01B1/04 ,  H01B1/02

摘要： A porous conductive thin film includes a layer of metal nanoparticles decorated on a layer of conductive carbon nanomaterials. The thin film can be supported by a porous support. The porous support can be a MCE paper upon which a metallic or semi-metallic single-walled carbon nanotube (SWCNT) layer is decorated with gold nanoparticles (AuNPs) or platinum nanoparticles (PtNPs). The thin film can be constructed by filtering a dispersion of SWCNTs onto MCE filter paper followed by the filtration of a citrate stabilized dispersion of AuNPs or PtNPs onto the SWCNT layer.

摘要翻译： 多孔导电薄膜包括在导电碳纳米材料层上装饰的一层金属纳米颗粒。 薄膜可以由多孔载体支撑。 多孔载体可以是MCE纸，金属或半金属单壁碳纳米管（SWCNT）层用金纳米颗粒（AuNP）或铂纳米颗粒（PtNP）进行装饰。 可以通过将SWCNT的分散体过滤到MCE滤纸上，然后将AuNP或PtNP的柠檬酸盐稳定分散体过滤到SWCNT层上来构建薄膜。

公开(公告)号：US07803542B2

公开(公告)日：2010-09-28

申请号：US11564674

申请日：2006-11-29

申请人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

发明人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6825 ,  G01N27/3277 ,  C12Q2565/133 ,  C12Q2563/113 ,  C12Q2525/205 ,  C12Q2537/163 ,  C12Q2537/137 ,  C12Q2565/525 ,  C12Q2565/549 ,  C12Q2565/607

摘要： The invention provides a general “signal-on” architecture for oligonucleotide-based detectors that leads to order of magnitude increases in signal gain and sensitivity as compared to prior art detectors. The detectors of the invention rely on base pairing between two oligonucleotide strands, the sensor strand and the blocker strand. In the ‘off’ position of the detector, i.e., in the absence of target, the blocker strand and sensor strand are base-paired. As shown in FIG. 1, the formation of comparatively rigid, duplex DNA prevents the redox moiety from approaching the electrode surface, thereby suppressing Faradaic currents. When target is added to the system, the target displaces the blocker strand, binds to the sensor strand, liberating the end of the redox-labeled oligonucleotide to produce a flexible element. This, in turn, allows the redox moiety to collide with the electrode surface, producing a readily detectable Faradaic current.

摘要翻译： 本发明提供了一种基于寡核苷酸的检测器的通用“信号启动”架构，与现有技术的检测器相比，信号增益和灵敏度的幅度增加了一个数量级。 本发明的检测器依赖于两个寡核苷酸链之间的碱基配对，传感器链和阻断链。 在检测器的“关闭”位置，即在没有靶的情况下，阻滞链和传感器链是碱基配对的。 如图所示。 1，相对刚性的双链DNA的形成防止氧化还原部分接近电极表面，从而抑制法拉第电流。 当将靶标加入到系统中时，靶标取代阻断链，结合传感器链，释放氧化还原标记的寡核苷酸的末端以产生柔性元件。 这又使得氧化还原部分与电极表面碰撞，产生易于检测的法拉第电流。

公开(公告)号：US07468459B2

公开(公告)日：2008-12-23

申请号：US10549425

申请日：2004-03-15

申请人： Yi Xiao ,  Joseph D. Armstrong, III ,  Shane W. Krska ,  Eugenia Njolito ,  Nelo R. Rivera ,  Yongkui Sun ,  Thorsten Rosner

发明人： Yi Xiao ,  Joseph D. Armstrong, III ,  Shane W. Krska ,  Eugenia Njolito ,  Nelo R. Rivera ,  Yongkui Sun ,  Thorsten Rosner

IPC分类号： C07C211/00 ,  C07D249/00

CPC分类号： B01J31/2295 ,  B01J31/24 ,  B01J31/2409 ,  B01J2231/645 ,  B01J2531/0263 ,  B01J2531/0286 ,  B01J2531/822 ,  B01J2531/842 ,  C07C227/16 ,  C07C231/12 ,  C07C231/18 ,  C07D487/04 ,  C07C237/20

摘要： The present invention relates to a process for the efficient preparation of enantiomerically enriched beta amino acid derivatives which are useful in the asymmetric synthesis of biologically active molecules. The process comprises an enantioselective hydrogenation of a prochiral beta amino acrylic acid derivative substrate in the presence of a transition metal precursor complexed with a chiral ferrocenyl diphosphine ligand.

摘要翻译： 本发明涉及有效制备对映体富集的β氨基酸衍生物的方法，其可用于生物活性分子的不对称合成。 该方法包括前手性β-氨基丙烯酸衍生物底物在与手性二茂铁基二膦配体络合的过渡金属前体存在下的对映选择性氢化。

公开(公告)号：US20070154909A1

公开(公告)日：2007-07-05

申请号：US11564674

申请日：2006-11-29

申请人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

发明人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6825 ,  G01N27/3277 ,  C12Q2565/133 ,  C12Q2563/113 ,  C12Q2525/205 ,  C12Q2537/163 ,  C12Q2537/137 ,  C12Q2565/525 ,  C12Q2565/549 ,  C12Q2565/607

摘要： The invention provides a general “signal-on” architecture for oligonucleotide-based detectors that leads to order of magnitude increases in signal gain and sensitivity as compared to prior art detectors. The detectors of the invention rely on base pairing between two oligonucleotide strands, the sensor strand and the blocker strand. In the ‘off’ position of the detector, i.e., in the absence of target, the blocker strand and sensor strand are base-paired. As shown in FIG. 1, the formation of comparatively rigid, duplex DNA prevents the redox moiety from approaching the electrode surface, thereby suppressing Faradaic currents. When target is added to the system, the target displaces the blocker strand, binds to the sensor strand, liberating the end of the redox-labeled oligonucleotide to produce a flexible element. This, in turn, allows the redox moiety to collide with the electrode surface, producing a readily detectable Faradaic current.

摘要翻译： 本发明提供了一种基于寡核苷酸的检测器的通用“信号启动”架构，与现有技术的检测器相比，信号增益和灵敏度的幅度增加了一个数量级。 本发明的检测器依赖于两个寡核苷酸链之间的碱基配对，传感器链和阻断链。 在检测器的“关闭”位置，即在没有靶的情况下，阻滞链和传感器链是碱基配对的。 如图所示。 1，相对刚性的双链DNA的形成防止氧化还原部分接近电极表面，从而抑制法拉第电流。 当将靶标加入到系统中时，靶标取代阻断链，结合传感器链，释放氧化还原标记的寡核苷酸的末端以产生柔性元件。 这又使得氧化还原部分与电极表面碰撞，产生易于检测的法拉第电流。

公开(公告)号：US11656235B2

公开(公告)日：2023-05-23

申请号：US17396663

申请日：2021-08-07

申请人： Yi Xiao ,  Obtin Alkhamis ,  Haixiang Yu

发明人： Yi Xiao ,  Obtin Alkhamis ,  Haixiang Yu

IPC分类号： G01N33/94 ,  G01N21/78 ,  G01N33/58

CPC分类号： G01N33/9486 ,  G01N21/78 ,  G01N33/583 ,  G01N33/946 ,  G01N33/948 ,  G01N33/9413

摘要： The subject invention provides materials and methods for single-step detection of target molecules in a sample. The methods and assays of the subject invention employ a dye-displacement strategy, in which aptamers complexed with a cyanine dye for sensitive and rapid detection of targets of interest. In the presence of a target, aptamer-target binding liberates the non-covalently bound aptamer-binding dye, resulting in optical changes that can be observed spectrophotometrically or with the naked eye. The methods and assays of the subject invention enable the colorimetric detection of targets of interest regardless of their structure, sequence, target-binding affinity, and physicochemical properties of their targets.

公开(公告)号：US20230136219A1

公开(公告)日：2023-05-04

申请号：US17512697

申请日：2021-10-28

申请人： Xiangyang Zhang ,  Yi Xiao

发明人： Xiangyang Zhang ,  Yi Xiao

IPC分类号： A45C1/06 ,  A45C11/18 ,  A45C13/18

摘要： The invention discloses a traceable card holder, comprising a first clamp plate, a second clamp plate, an elastic band, top cover components, bottom cover components, a tracker and an elastic clamp; the first clamp plate is arranged oppositely to the second clamp plate, the elastic band is connected respectively with the first clamp plate and the second clamp plate, and the elastic band is used to drive the first clamp plate and the second clamp plate to move towards each other for clamping; the top cover components are detachably mounted on one side of the first clamp plate which is away from the second clamp plate, and the tracker is detachably mounted on the top cover components.

公开(公告)号：US10683507B2

公开(公告)日：2020-06-16

申请号：US15900061

申请日：2018-02-20

申请人： Yi Xiao ,  Haixiang Yu

发明人： Yi Xiao ,  Haixiang Yu

IPC分类号： C12N15/11 ,  C12N15/115 ,  C09B23/01 ,  C07C225/10 ,  G01N33/50

摘要： The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.

公开(公告)号：US10550395B2

公开(公告)日：2020-02-04

申请号：US15947315

申请日：2018-04-06

申请人： Yi Xiao ,  Haixiang Yu

发明人： Yi Xiao ,  Haixiang Yu

IPC分类号： C12N15/115 ,  G01N33/94 ,  G01N33/92

摘要： The subject invention provides methods, assays and products for detecting small-molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target in a sample comprises providing a sample, contacting the sample with an aptamer-based sensor selective for the small-molecule target, and sensitively and rapidly detecting the small-molecule target in the sample. Specifically, the method utilizes EATR-amplified small-molecule sensors based on cooperative binding split aptamers (CBSAs).